Acid-Fast Stain Overview
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Acid-Fast Stain Overview

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Questions and Answers

What are mycolic acids primarily composed of?

  • Short chain fatty acids
  • Carbohydrate polymers
  • Protein complexes
  • Long chain a-alkyl, -hydroxy fatty acids (correct)

    • How do mycolic acids influence the pathogenicity of certain microorganisms?

  • They promote rapid growth of the bacteria.
  • They inhibit replication of bacterial DNA.
  • They enhance nutrient absorption through the cell wall.
  • They protect bacteria from antibiotics and phagocytosis. (correct)

    • What is the primary stain used in the acid-fast staining procedure?

  • Crystal violet
  • Loeffler's alkaline methylene blue
  • Safranin
  • Kinyoun's carbolfuchsin (correct)

    • What color do acid-fast positive organisms appear after the acid-fast staining procedure?

    <p>Red</p> Signup and view all the answers

    What happens to non-acid-fast organisms during the decolorization step of acid-fast staining?

    <p>They are decolorized.</p> Signup and view all the answers

    Which of the following genera includes acid-fast bacteria?

    <p>Mycobacterium</p> Signup and view all the answers

    What kind of bacteria is characterized by having a cell wall that includes mycolic acids?

    <p>Acid-fast bacteria</p> Signup and view all the answers

    Which stain is used as a counterstain in the acid-fast staining process?

    <p>Loeffler's alkaline methylene blue</p> Signup and view all the answers

    Why do acid-fast bacteria exhibit extremely slow growth?

    <p>Their cell walls inhibit nutrient uptake.</p> Signup and view all the answers

    Which of the following is a disadvantage of the thick cell wall of acid-fast bacteria?

    <p>Slower growth rate</p> Signup and view all the answers

    Study Notes

    Acid-Fast Stain

    • Acid-fast bacteria are gram-positive bacteria that contain up to 60% mycolic acids in their cell walls.
    • Mycolic acids are long chain a-alkyl, -hydroxy fatty acids.
    • Mycolic acids protect against some antibiotics and phagocytosis by macrophages.
    • Mycolic acids make it difficult for nutrients to enter the cell, resulting in slow growth for acid-fast bacteria.
    • Acid-fast bacteria resist most stains, including the Gram stain.
    • The acid-fast stain differentiates between acid-fast positive and acid-fast negative bacteria.
    • The primary stain in the acid-fast stain is Kinyoun's carbolfuchsin, which stains acid-fast positive organisms red.
    • Acid-fast positive bacteria retain the red stain after decolorization with acid alcohol.
    • Acid-fast negative bacteria are decolorized by acid alcohol and are stained blue by Loeffler's alkaline methylene blue.
    • Genera Mycobacterium, Nocardia, and Corynebacterium include pathogens.
    • The acid-fast stain helps identify pathogens in clinical samples.

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    Acid Fast Stain Exercise 20 PDF

    Description

    This quiz covers the principles and significance of the acid-fast stain in microbiology. Learn about the characteristics of acid-fast bacteria, the staining process, and specific pathogens related to this staining technique. Ideal for those studying bacteriology and microbiological techniques.

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