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What is the primary function of the Na*-K+ ATPase pump?

  • To generate ATP directly for cellular energy
  • To transport Na* ions into the cell
  • To maintain Na* and K+ concentration gradients (correct)
  • To transport K+ ions out of the cell
  • Which characteristic distinguishes primary active transport from secondary active transport?

  • It exclusively transports ions
  • It transports substances in only one direction
  • It involves the direct use of ATP (correct)
  • It operates only in nerve cells
  • In primary active transport via the Na*-K+ pump, what is the ratio of Na* being pumped out to K+ being pumped in?

  • 2:3
  • 1:1
  • 3:1
  • 3:2 (correct)
  • What role does the Na* concentration gradient play in secondary active transport?

    <p>It provides energy for transporting other solutes uphill (A)</p> Signup and view all the answers

    What enhances the activity of the Na*-K+ pump during cellular swelling?

    <p>Osmoregulation processes (A)</p> Signup and view all the answers

    Which statement defines the nature of the countertransport or antiport system?

    <p>One substance is transported one way while another substance is transported the opposite way (D)</p> Signup and view all the answers

    What is a key characteristic of secondary active transport?

    <p>It couples the movement of multiple solutes (C)</p> Signup and view all the answers

    What is one major consequence of maintaining cellular ion concentrations through active transport?

    <p>Prevention of osmotic swelling (D)</p> Signup and view all the answers

    What is the role of local regulators in the process of paracrine signaling?

    <p>They diffuse through fluid to nearby target cells. (C)</p> Signup and view all the answers

    What occurs during autocrine signaling?

    <p>Cells act on themselves by releasing a local regulator. (B)</p> Signup and view all the answers

    Which type of signaling is characterized by neurotransmitter molecules diffusing across a synapse?

    <p>Synaptic signaling (B)</p> Signup and view all the answers

    What is a key difference between local signaling and long-distance signaling?

    <p>Local signaling affects only nearby cells. (C)</p> Signup and view all the answers

    What happens to receptor sensitivity during down regulation?

    <p>The number of receptors decreases. (C)</p> Signup and view all the answers

    What triggers the secretion of neurotransmitter molecules in synaptic signaling?

    <p>An electrical signal along a nerve cell. (D)</p> Signup and view all the answers

    What can cause a decrease in receptor synthesis according to cellular needs?

    <p>The presence of a signaling molecule in excess. (D)</p> Signup and view all the answers

    Which of the following is NOT a mechanism of signaling discussed?

    <p>Electrical signaling (A)</p> Signup and view all the answers

    What is the primary function of receptor-mediated endocytosis?

    <p>To allow cells to take in specific molecules with minimal fluid intake. (A)</p> Signup and view all the answers

    What triggers the process of exocytosis in cells?

    <p>An increase in intracellular calcium levels. (D)</p> Signup and view all the answers

    Which of the following accurately describes total body water distribution?

    <p>Newborns have the highest percentage of total body water. (B)</p> Signup and view all the answers

    Which ion is the major cation found in extracellular fluid?

    <p>Na+ (A)</p> Signup and view all the answers

    What is one potential fate of molecules released during exocytosis?

    <p>They may attach to the outer surface of the cell. (A)</p> Signup and view all the answers

    What characterizes the fluid found in the intracellular compartment?

    <p>It predominantly contains potassium and magnesium ions. (B)</p> Signup and view all the answers

    In receptor-mediated endocytosis, which occurs after a specific molecule binds to a receptor?

    <p>The membrane forms a pit that soon pinches off. (A)</p> Signup and view all the answers

    What percentage of total body water is intracellular fluid?

    <p>About 2/3 (B)</p> Signup and view all the answers

    What role do anions play in Donnan's equilibrium?

    <p>They exert osmotic pressure causing water to enter the capillaries. (B)</p> Signup and view all the answers

    What is the primary function of the receptors in homeostasis?

    <p>To send signal information to the control center. (A)</p> Signup and view all the answers

    What determines the resting membrane potential (RMP)?

    <p>The concentration of K+ inside and outside the cell. (A)</p> Signup and view all the answers

    How does the control center respond to changes detected by the receptors?

    <p>By generating signal commands necessary for correction. (B)</p> Signup and view all the answers

    What is the function of the efferent pathways in the homeostatic mechanism?

    <p>To transmit commands from the control center to effector organs. (D)</p> Signup and view all the answers

    What best describes homeostasis?

    <p>A mechanism that maintains the internal environment within narrow limits. (A)</p> Signup and view all the answers

    Which of the following components is NOT part of the homeostatic mechanism?

    <p>Feedback loops (A)</p> Signup and view all the answers

    What is the significance of the set point in homeostasis?

    <p>It is the point the body strives to maintain for normal functioning. (A)</p> Signup and view all the answers

    What is the primary mechanism involved in receptor down regulation?

    <p>Transporting receptors to lysosomes for destruction (C)</p> Signup and view all the answers

    What is the main purpose of the transduction stage in cell signaling?

    <p>To convert the signal into a form that triggers a cellular response (D)</p> Signup and view all the answers

    Which of the following is a characteristic of G protein-linked receptors?

    <p>They require G protein for signal transduction (B)</p> Signup and view all the answers

    What happens when a ligand binds to ion channel-linked receptors?

    <p>The channel either opens or closes to regulate ion flow (D)</p> Signup and view all the answers

    Which category of responses is NOT typically triggered by cell signaling?

    <p>Inhibition of hormone synthesis (A)</p> Signup and view all the answers

    In what location are intracellular receptors typically found?

    <p>In the cytoplasm or nucleus of target cells (D)</p> Signup and view all the answers

    Which receptor type functions by catalyzing the transfer of a phosphate group to tyrosine?

    <p>Enzyme-linked receptors (Receptor tyrosine kinases) (D)</p> Signup and view all the answers

    What type of molecules typically bind to intracellular receptors?

    <p>Small, hydrophobic molecules (C)</p> Signup and view all the answers

    What initiates the release of Ca** from the endoplasmic reticulum or sarcoplasmic reticulum into the cytosol?

    <p>IP3 (B)</p> Signup and view all the answers

    What is the role of DAG in cellular signaling?

    <p>It activates protein kinase C (PKC) (C)</p> Signup and view all the answers

    What happens when Ca** binds to calmodulin?

    <p>It forms a Ca**-calmodulin complex that activates protein kinases (B)</p> Signup and view all the answers

    What is meant by signal amplification in a signaling pathway?

    <p>One hormone molecule leads to the production of a large number of product molecules (D)</p> Signup and view all the answers

    Which of the following describes a mechanism for signal termination?

    <p>Hydrolysis of GTP to GDP by GTPase (A)</p> Signup and view all the answers

    Why is signal termination important in cellular signaling?

    <p>To allow molecules to respond to new signals (D)</p> Signup and view all the answers

    What effect does the cholera toxin have on cellular signaling?

    <p>It activates G proteins causing them to continuously stimulate adenylyl cyclase (A)</p> Signup and view all the answers

    What is the consequence of failed signal termination in cellular pathways?

    <p>Possible severe physiological effects, such as diarrhea (D)</p> Signup and view all the answers

    Flashcards

    Primary Active Transport

    Movement of molecules against their concentration gradient, requiring direct energy input (ATP).

    Na+-K+ Pump

    A primary active transport protein that moves Na+ out of and K+ into a cell, establishing concentration gradients.

    Secondary Active Transport

    Movement of molecules against their concentration gradient, using the energy from another molecule moving down its gradient (often Na+).

    Electrochemical Gradient

    A gradient of both concentration and electrical charge that drives the movement of ions.

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    Countertransport (Antiport)

    A type of transport where two substances move in opposite directions across the membrane.

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    Na+-sugar transporters

    Transmembrane proteins that facilitate the movement of Na+ and sugars (glucose, mannose, galactose) across the cell membrane.

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    Active transport

    The movement of molecules across a cell membrane against their concentration gradient, requiring energy.

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    Non-specific membrane transport

    A type of membrane transport that takes in all dissolved solutes in a droplet; common in most cells.

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    Receptor-mediated endocytosis

    A selective method of membrane transport where cells take in specific molecules by binding them to receptors, forming pits and vesicles.

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    Exocytosis

    The process by which cells release substances to the exterior.

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    Total body water (TBW)

    The total amount of water in the human body, approximately 60% of body weight.

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    Intracellular fluid (ICF)

    Fluid inside cells, constituting about 2/3 of total body water.

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    Extracellular fluid (ECF)

    Fluid outside cells, constituting about 1/3 of total body water.

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    Intravascular fluid (plasma)

    The fluid part of blood, a component of ECF.

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    Interstitial fluid

    ECF found between cells.

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    Major cation of ICF

    Potassium (K+)

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    Major anion of ICF

    Proteins and organic phosphates (ATP, ADP, AMP).

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    Major cation of ECF

    Sodium (Na+)

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    Major anions of ECF

    Chloride (Cl-) and bicarbonate (HCO3-).

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    Donnan's equilibrium

    Imbalance in ion concentrations across a membrane due to charged molecules.

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    Osmosis

    Movement of water across a membrane from a region of higher water concentration to a region of lower water concentration.

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    Interstitial fluid

    Fluid surrounding cells, acting as the internal environment for cells.

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    Homeostasis

    Maintaining a stable internal environment despite external changes.

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    Receptor (homeostasis)

    Detects changes in the internal or external environment, sending signals to the control center.

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    Afferent pathway

    The pathway that carries signals from the receptor to the control center.

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    Control center (homeostasis)

    Evaluates received signals, determines appropriate response, and sends commands.

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    Efferent pathway

    Pathway carrying signals from the control center to effector organs.

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    Effector organ

    Organ that carries out the instructions received from the control center to counteract the change in the internal environment.

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    Set point

    A particular value or range that the body maintains in homeostasis.

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    Cell-cell recognition

    Animal cells with specific surface molecules dock to each other, initiating cell communication.

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    Local signaling

    A cell releases a signal molecule that diffuses to nearby target cells, triggering a response.

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    Paracrine signaling

    Local signaling where a cell releases a signal (local regulator) that affects nearby target cells.

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    Autocrine signaling

    Local signaling where a cell releases a signal that affects the cell that released it.

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    Synaptic signaling

    Specialized local signaling in the nervous system, using neurotransmitters to transmit signals across synapses.

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    Long-distance signaling

    A controlling cell releases hormones into the circulatory system to affect target cells far away.

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    Hormone

    A long-distance signaling molecule released by a controlling cell.

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    Reception (Cell Signaling)

    Target cell detection of a signaling molecule, usually binding to a receptor.

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    Receptor Protein

    A protein on the cell surface or inside the cell that binds a signaling molecule.

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    Down Regulation

    Decreasing the number of receptors in response to high ligand levels, reducing the cell's sensitivity.

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    Receptor Downregulation

    A process where the number of receptors on a cell decreases, often due to receptor-recycling in lysosomes.

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    Signal Transduction

    The conversion of a signal from one form to another within a cell to trigger a response.

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    Transduction Pathway

    A series of molecules that a signal travels through to cause specific cellular responses.

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    Cell Surface Receptors

    Proteins embedded in the cell membrane that bind to signaling molecules.

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    G Protein-Coupled Receptors (GPCRs)

    Cell surface receptors that use a G protein to activate other enzymes in the cell.

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    Receptor Tyrosine Kinases (RTKs)

    Cell surface receptors that activate enzymes inside the cell when a signaling molecule binds.

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    Ion Channel-Linked Receptors

    Cell surface receptors that open or close channels allowing ions to pass through the cell membrane.

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    Intracellular Receptors

    Receptors located inside the cell, typically in the cytoplasm or nucleus.

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    Cellular Response

    The outcome of a transduction pathway; the changes that occur in the cell.

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    IP3's role

    IP3 triggers calcium release from the endoplasmic reticulum or sarcoplasmic reticulum into the cytosol, impacting intracellular processes.

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    DAG's effect

    DAG activates protein kinase C (PKC), leading to protein phosphorylation and subsequent changes in cell function.

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    Ca2+-calmodulin system

    Calcium binds to calmodulin, forming a complex that activates protein kinases, influencing various biological responses.

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    Signal amplification

    A small signal from a chemical messenger can produce a larger response in a target cell.

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    Signal termination

    The process of stopping the signal pathway after the response is achieved.

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    GTP hydrolysis

    The process where a GTPase enzyme converts GTP to GDP, deactivating the G protein.

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    cAMP inactivation

    cAMP is quickly broken down by a phosphodiesterase into AMP, ending its signaling effects.

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    Significance of signal termination

    Termination allows the system to respond to new signals and prevents prolonged or unwanted effects.

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    Hormone action on cytoplasmic Ca²⁺

    Hormones can increase cytoplasmic calcium through activation of calcium channels and release from ER or mitochondria.

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    Cholera toxin effect

    Cholera toxin modifies a G protein so it cannot switch off, resulting in prolonged cAMP stimulation, severe diarrhea.

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    Study Notes

    Transport Across Cell Membranes

    • The plasma membrane acts as a barrier between the extracellular fluid (ECF) and the cytosol of cells.
    • Substances continuously exchange between the cytosol and ECF.
    • ECF contains nutrients (glucose, amino acids, fatty acids), water, ions, oxygen, vitamins, and macromolecules needed by the cell.
    • Cytosol contains waste products (carbon dioxide) and manufactured substances that need to exit the cell.
    • Cell and body survival depend on the transport of these substances.
    • Membrane transport is the process substances use to enter and exit the cell.
    • Membrane transport is categorized into passive and active processes, based on the cell's energy expenditure.

    Passive Processes

    • Do not require cellular energy.
    • Substances move down their concentration gradient.

    Diffusion

    • Simple diffusion: movement of small, nonpolar solutes across the phospholipid bilayer (e.g., oxygen, carbon dioxide, steroids).
    • Facilitated diffusion: the movement of small polar or charged solutes facilitated by transport proteins (e.g., ion channels or carrier proteins).

    Osmosis

    • Special case of diffusion; the movement of water across a selectively permeable membrane from a region of high water concentration to a region of low water concentration.

    Active Processes

    • Require cellular energy (ATP).
    • Substances move against their concentration gradient.

    Active Transport

    • Primary active transport: uses ATP directly to move substances against their concentration gradient (e.g., Na+-K+ pump).
    • Secondary active transport: uses the electrochemical gradient established by primary active transport to move substances against their concentration gradient (e.g., Na+-glucose cotransporter).

    Vesicular Transport

    • Involves vesicles, small membranous sacs.
    • Endocytosis: taking substances into the cell.
    • Exocytosis: releasing substances from the cell.

    Passive Diffusion

    • No energy is required.
    • Movement is down the concentration/electrochemical gradient.
    • Solutes that are small or nonpolar move into/out of a cell passively.

    Facilitated Diffusion

    • Movement is down the concentration/electrochemical gradient.
    • Requires a transport protein.
    • Small polar or charged solutes move across the membrane with help of protein channels.
    • Carrier-mediated diffusion: A solute binds to a carrier on one side of the membrane; the carrier changes shape, moving the solute to the other side before returning to its original shape.

    Saturation

    • In simple diffusion, the rate increases proportionally to the concentration.
    • In facilitated diffusion, the initial increase is proportional, but the rate reaches a limit (transport maximum, Tm) when all transport proteins are occupied.

    Competition

    • In facilitated diffusion, similarly shaped molecules can compete for the same binding site on the carrier protein.
    • When molecule A increases, it decreases B's transport, and vice versa.
    • No competition in simple diffusion.

    Specificity

    • Carrier proteins show high specificity, transporting only certain molecules (e.g., a glucose transporter doesn't carry fructose).

    Types of carrier proteins

    • Uniport: carries one substance.
    • Co-transport (symport): carries two substances in the same direction.
    • Counter-transport (antiport): carries one substance in one direction and another substance in the opposite direction.

    Active Transport - Primary

    • Direct expenditure of energy (ATP).
    • Establishes and maintains concentration gradients.
    • Example: Na⁺-K⁺ pump

    Active Transport - Secondary

    • Uses electrochemical gradients created by primary active transport to move substances against concentration gradient.
    • Example: glucose transport in intestine.

    Vesicular Transport

    • Bulk transport using membrane-bound vesicles.
    • Endocytosis: taking in material.
    • Exocytosis: releasing material from the cell.

    Membrane transport

    • Includes passive and active processes
    • Passive processes (diffusion and osmosis) do not require energy expenditure
    • Active processes (active and vesicular transport) require energy.
    • Cell survival, function, and appropriate responses to outside stimuli depend on membrane transport.

    Body Fluids

    • Water is essential for all life.
    • About 60% of an adult's weight is water.
    • Water is distributed between intracellular and extracellular compartments.

    Cellular Communication

    • Cells communicate to maintain homeostasis (internal stability) by sending and receiving signals.
    • Cells communicate with each other through direct and/or long distance signaling methods.
    • Types of communication:
      • Direct contact (Gap junctions): adjacent cells have channels connecting cytoplasms.
      • Local signaling (paracrine and autocrine): signals travel short distances.
      • Long distance signaling (hormones): signals travel through the circulatory system.

    Stages of Cell Signaling

    • Reception: signaling molecule (ligand) binds to a receptor protein.
    • Transduction a series of changes to convert the signal (e.g., activating enzymes).
    • Response: resulting cellular action (e.g., altering gene expression).
    • Signal termination: the signal in the cell must be terminated.

    Homeostasis

    • Cells and organisms need internal stability.
    • Homeostatic mechanisms are important for survival.
    • Receptors, Afferent pathways, Control centers, Efferent pathways and Effectors are components of the homeostasis mechanism.

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