Salmonella and Pasteurella (PDF)

BACTERIOLOGY AND MYCOLOGY Overview of bacterial species Salmonella spp. salmonellosis Overview of bacterial species Enterobacteriacaei: large family of gram neg bacteria, entercolitis: inflammation of small intestine, septicaemia:blood poisoning, enteric fever aka typhiod fever Overview of ba...

BACTERIOLOGY AND MYCOLOGY Overview of bacterial species Salmonella spp. salmonellosis Overview of bacterial species Enterobacteriacaei: large family of gram neg bacteria, entercolitis: inflammation of small intestine, septicaemia:blood poisoning, enteric fever aka typhiod fever Overview of bacterial species  TSIA TEST Triple-sugar iron agar (TSI agar) is another example of a multi-test agar. It tests for the fermentation, with or without gas production, of glucose, lactose, and sucrose. It also tests for the production of hydrogen sulfide from amino acids. Phenol red is the pH indicator used in this test medium. Procedure 1. Obtain a slant of TSIA. 2. Using an inoculating needle, stab your assigned organism into the butt of the TSIA slant. As you remove the inoculating needle, drag it in a zigzag pattern up the surface of the slant portion of the tube. 3. Incubate the slant for 24-48 hours. 4. After the incubation period, record any changes in the tube. Interpretation Review your results from Carbohydrate Fermentation and note the following: Slant color/butt color: Slant color indicates the fermentation of lactose and/or sucrose. Butt color indicates the fermentation of glucose. Production of gas: Agar shows bubbles or may split. Production of H2S: H2S formation is indicated by a blackening of the medium. TSIA TEST RESULTS ONPG: o‐nitrophenyl‐β‐D‐galactopyranoside (ONPG) testCoupled with the lactose fermentation test, it can also determine the ability of bacteria to synthesize the permease enzyme. It is used in the biochemical characterization and identification of Gram-negative bacteria. KCN: Potassium cyanide test.  Widal Test The Widal blood test is used to diagnose typhoid fever. It is a blood test that measures the presence and level of antibodies against two strains of Salmonella typhi, the bacteria that cause typhoid fever. During the test, whole blood from a patient is mixed with an antiserum containing antibodies against Salmonella typhi. If there are antibodies present in the blood sample from the patient, they will react with the antiserum and cause clumping or agglutination of red blood cells. This indicates that the person has typhoid fever. Isolation/Identification Overview of bacterial species Pasteurella spp. Haemorrhagic septicaemia Overview of bacterial species Atrophic rhinitis is nasal dryness that occurs when tissue inside of your nose thins or atrophies. Pleurisy, also known as pleuritis, is inflammation of the membranes that surround the lungs and line the chest cavity (pleurae). This can result in a sharp chest pain while breathing. Occasionally the pain may be a constant dull ache.