Principle of RNA Isolation PDF
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This document describes the principle and procedure of RNA isolation. It details the use of Trizol, phenol, and chloroform for separating RNA from DNA and proteins. The procedure involves several steps including extraction, centrifugation, and precipitation with isopropanol.
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**Principle of RNA Isolation** Total RNA is isolated and separated from DNA and protein after extraction with a solution called as Trizol. Trizol is an acidic solution, phenol and chloroform. Trizol irreversibly denatures proteins and RNases. This is followed by centrifugation. Under acidic conditi...
**Principle of RNA Isolation** Total RNA is isolated and separated from DNA and protein after extraction with a solution called as Trizol. Trizol is an acidic solution, phenol and chloroform. Trizol irreversibly denatures proteins and RNases. This is followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol. **Materials Required for RNA Isolation** - - - - - - **Procedure of RNA Isolation** 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.
