II B.Pharm - Pharmaceutical Microbiology MCQ100.pdf

Subject: Pharmaceutical Microbiology - Dr. Rajeshkumar Sl No Questions Ans Reference Difficulty* Chapter No 1 Who showed that open broth tubes w...

Subject: Pharmaceutical Microbiology - Dr. Rajeshkumar Sl No Questions Ans Reference Difficulty* Chapter No 1 Who showed that open broth tubes were free of bacteria when air was free of b Pharmaceutical Microbiology, Kar, D Unit I dust Ashutosh, New Age International, 2007 a. Abbc Spallanzani b. John Tyndall c. Francisco Redi d. Pasteur 2 Father of microbiology c Pharmaceutical Microbiology, Kar, E Unit I a. Louis Pasteur Ashutosh, New Age International, 2007 b. Lister c. A.V. Leeuwenhock d. Robert Koch 3 The antiseptic method was demonstrated by b Pharmaceutical Microbiology, Kar, MD Unit I a. Lwanowski Ashutosh, New Age International, 2007 b. Lord Lister c. Edward Jenner d. Beijerinck 4 Small pox vaccine was discovered by d Pharmaceutical Microbiology, Kar, E Unit I a. Robert Koch Ashutosh, New Age International, 2007 b. Louis Pasteur c. Lister d. Edward Jenner 5 In a fluorescent microscope the objective lens is made of c Molecular Biology of the Cell, Bruce D Unit I a. Glass Alberts, Edition 6, Garland Science b. Quartz Publisher, 2017 c. Polythene d. Plastic 6 Father of Medical Microbiology c Pharmaceutical Microbiology, Kar, E Unit I a. Pasteur Ashutosh, New Age International, 2007 b. Jenner c. Koch d. A.L.Hock 7 The following is the main feature of prokaryotic organism b Molecular Biology of the Cell, Bruce Unit I a. Absence of locomotion Alberts, Edition 6, Garland Science b. Absence of nuclear envelope Publisher, 2017 c. Absence of nuclear material d. Absence of protein synthesis 8 Antiseptic method was first introduced by a Pharmaceutical Microbiology, Kar, MD Unit I a. Lord Lister Ashutosh, New Age International, 2007 b. Iwanowski c. Beijernick d. Edward Jenner 9 The resolution power of the compound microscope is a Molecular Biology of the Cell, Bruce D Unit I a. 0.2 micron Alberts, Edition 6, Garland Science b. 0.2 millimeter Publisher, 2017 c. 0.2 Angstrom units d. 0.2 centimeter 10 The functions of plasmid are d Molecular Biology of the Cell, Bruce MD Unit I a. DNA replication Alberts, Edition 6, Garland Science b. Protein synthesis Publisher, 2017 c. Cell wall synthesis d. Motility 11 Endotoxin produced by gramnegative bacteria is present in b Molecular Biology of the Cell, Bruce MD Unit I a. Peptidoglycan Alberts, Edition 6, Garland Science b. Lippolysacharide Publisher, 2017 c. Theichoic acid d. Inner membrane 12 Which one of the following was Gramnegative, chemolithotrophic bacteria? b Molecular Biology of the Cell, Bruce Unit I a. Siderococcus Alberts, Edition 6, Garland Science b. E.coli Publisher, 2017 c. Spirellum d. Mycoplasms 13 The bacterial pili mainly contain c Molecular Biology of the Cell, Bruce MD Unit I a. Carbohydrates Alberts, Edition 6, Garland Science b. Lipids Publisher, 2017 c. Proteins d. Minerals 14 Shape of the vibrio bacteria a Molecular Biology of the Cell, Bruce E Unit I a. Comma Alberts, Edition 6, Garland Science b. Spherical Publisher, 2017 c. Rod d. No proper shape 15 Log Phase is important for production of a Pharmaceutical Microbiology, Kar, E Unit I a. Antibiotics Ashutosh, New Age International, 2007 b. Aminoacids c. Toxins d. Vitamins 16 Meosomes are the part of a Pharmaceutical Microbiology, Kar, MD Unit I a. Plasma membrane Ashutosh, New Age International, 2007 b. ER c. Lysosomes d. Golgi 17 All prokaryotes are surrounded by a cell wall except a Pharmaceutical Microbiology, Kar, MD Unit I a. Mycoplasms Ashutosh, New Age International, 2007 b. Sperochetes c. Actinomycetes d. Methanogena 18 Spirochete is an example for ------------------- shaped bacteria b Pharmaceutical Microbiology, Kar, D Unit I a. Cocci Ashutosh, New Age International, 2007 b. Modified Rod c. Rod d. Modified Cocci 19 Cell wall of gram negative bacteria is d Pharmaceutical Microbiology, Kar, E Unit I a. Thick Ashutosh, New Age International, 2007 b. Contains only Lipids c. Contains no cell wall d. Thin 20 Fimbriae are used for a Pharmaceutical Microbiology, Kar, E Unit I a. Cell attachment Ashutosh, New Age International, 2007 b. Movement c. Respiration d. Protein synthesis 21 Catalase production is negative in which of the following? a Ananthanarayan and Paniker's D Unit II a.Streptococcus Textbook of Microbiology, reprint, b.Salmonella Orient Blackswan, 2006 c.Proteus d.Staphylococcus 22 Which of the following is a substitute for crystal violet used in gram-staining a Ananthanarayan and Paniker's MD Unit II procedure? Textbook of Microbiology, reprint, a.Methylene blue Orient Blackswan, 2006 b.Bromocresol green c.Safranin d.Phenolpthalene 23 The organisms that can be acid-fast stained is b Ananthanarayan and Paniker's MD Unit II a.Streptococcus Textbook of Microbiology, reprint, b.Tubercle bacilli Orient Blackswan, 2006 c.Spira bacilli d.E.coli 24 The primary stain of Gram's method is c Ananthanarayan and Paniker's E Unit II a.Safranin Textbook of Microbiology, reprint, b.Phenolpthalene Orient Blackswan, 2006 c.Crystal violet d.Methyl red 25 Which of the following is commonly used as Gram's decolouriser? a Ananthanarayan and Paniker's E Unit II a.Alcohol Textbook of Microbiology, reprint, b.Methylene Orient Blackswan, 2006 c.Acetone d.Crystal violet 26 What is the colour of gram -postive bacteria on a gram staining? a Ananthanarayan and Paniker's MD Unit II a.Purple Textbook of Microbiology, reprint, b.Pink Orient Blackswan, 2006 c.Colourless d.Green 27 What is the colour of gram-negative bacteria on a gram staining? b Ananthanarayan and Paniker's E Unit II a.Purple Textbook of Microbiology, reprint, b.Pink Orient Blackswan, 2006 c.Green d.Colourless 28 What is the order of reagents used in the Gram stain? b Ananthanarayan and Paniker's D Unit II a.Crystal violet, iodine, safranin, decolorizer Textbook of Microbiology, reprint, b.Crystal violet, iodine, decolorizer, safranin Orient Blackswan, 2006 c.Safranin, crystal violet, decolorizer, iodine d.Crystal violet, safranin, iodine, decolorizer 29 Which of the following articles can be sterilized in an autoclave? b Ananthanarayan and Paniker's E Unit II a.Pippets Textbook of Microbiology, reprint, b.Culture media Orient Blackswan, 2006 c.Petri Plates d.Powder 30 Which of the following is best to sterilize heat labile solutions? c Ananthanarayan and Paniker's E Unit II a.Dry heat Textbook of Microbiology, reprint, b.Autoclave Orient Blackswan, 2006 c.Membrane filtration d.Pasteurization 31 The time required to kill 90% of the microorganisms in a sample at a specific d Ananthanarayan and Paniker's D Unit II temperature is the Textbook of Microbiology, reprint, a.Decimal reduction time Orient Blackswan, 2006 b.Thermal death point c.F value d.D value 32 Milk is pasteurized in batch method by keeping it at a Ananthanarayan and Paniker's MD Unit II a.63°C for 30 minutes Textbook of Microbiology, reprint, b.72°C for 60 seconds Orient Blackswan, 2006 c.73°C for 30 minutes d.72°C for 6 minutes 33 Preservation of foods by using salts and sugars works by c Ananthanarayan and Paniker's MD Unit II a.Raising pH Textbook of Microbiology, reprint, b.lowering osmotic pressure Orient Blackswan, 2006 c.Creating a hypertonic environment d.Creating a hypotonic environment 34 Which of the following items could be sterilized by dry heat sterilization? c Ananthanarayan and Paniker's E Unit II a.Intravenous (IV) solution Textbook of Microbiology, reprint, b.Plastic IV bags Orient Blackswan, 2006 c.Glass pipettes d.Rubber gloves 35 Which of the following does not kill endospores? d Ananthanarayan and Paniker's MD Unit II a.Autoclave Textbook of Microbiology, reprint, b.Incineration Orient Blackswan, 2006 c.Hot air sterilization d.Pasteurization 36 Which of the following describes the effect of ionizing radiation on microbes? c Ananthanarayan and Paniker's D Unit II a.Changes the 3-dimensional structure of proteins to a 2-dimen-sional form Textbook of Microbiology, reprint, b.Substantially decreases enzymatic activity, slowing microbial reproduction Orient Blackswan, 2006 c.Induces electrons and protons to jump out of the molecules of the microbe d.Guanine Cytosine mutation occur 37 The process of making an object free from living organisms including bacterial d Ananthanarayan and Paniker's E Unit II and fungal spores and viruses is known as Textbook of Microbiology, reprint, a.Pasteurization Orient Blackswan, 2006 b.Antisepsis c.Disinfection d.Sterilization 38 Microbes can be removed from a liquid solution by the process of a Ananthanarayan and Paniker's E Unit II a.filtration Textbook of Microbiology, reprint, b.freeze-drying Orient Blackswan, 2006 c.osmosis d.desiccation 39 Which of the following is bactericidal? b Ananthanarayan and Paniker's MD Unit II a.Membrane filtration Textbook of Microbiology, reprint, b.Ionizing radiation Orient Blackswan, 2006 c.Freeze-drying d.Deep freezing 40 Temperature used in autoclave d Ananthanarayan and Paniker's MD Unit II a.191°C Textbook of Microbiology, reprint, b.171°C Orient Blackswan, 2006 c.151°C d.121°C 41 Which of the following disinfectants act by disrupting microbial membranes? a Ananthanarayan and Paniker's D Unit III a.Cationic detergents Textbook of Microbiology, reprint, b.Halogens Orient Blackswan, 2006 c.Heavy metals d.Aldehydes 42 Which of the following is not a disinfectant containing a heavy metal? d Ananthanarayan and Paniker's MD Unit III a.Silver nitrate Textbook of Microbiology, reprint, b.Mercurochrome Orient Blackswan, 2006 c.Copper sulfate d.Chlorine 43 Which of the following substances can sterilize? c Ananthanarayan and Paniker's MD Unit III a.Alcohol Textbook of Microbiology, reprint, b.Cetylpyridinium chloride Orient Blackswan, 2006 c.Ethylene oxide d.Chlorine 44 Which disinfectant was the most effective against Salmonella"? d Ananthanarayan and Paniker's D Unit III a.Phenol Textbook of Microbiology, reprint, b.Cetylpyridinium chloride Orient Blackswan, 2006 c.Hexachlorophene d.Merthiolate 45 Bactericidal concentration of phenol is d Ananthanarayan and Paniker's MD Unit III a.0.25% Textbook of Microbiology, reprint, b.0.5% Orient Blackswan, 2006 c.0.75% d.1% 46 All of the following are sporicidal except d Ananthanarayan and Paniker's E Unit III a.glutaraldehyde Textbook of Microbiology, reprint, b.ethylene oxide Orient Blackswan, 2006 c.formaldehyde d.alcohol 47 Which of the following was the first widely used antiseptic and disinfectant? b Ananthanarayan and Paniker's E Unit III a.Chlorine Textbook of Microbiology, reprint, b.Phenol Orient Blackswan, 2006 c.Iodine d.Alcohol 48 Iodophores are mixture of b Ananthanarayan and Paniker's E Unit III a.iodine and aldehydes Textbook of Microbiology, reprint, b.iodine and surface active agents Orient Blackswan, 2006 c.iodine and alcohols d.iodine and phenols 49 Which of the following compounds are commonly used as general antiseptics to c Ananthanarayan and Paniker's MD Unit III treat cuts and scratches? Textbook of Microbiology, reprint, a.Aldehydes Orient Blackswan, 2006 b.Ethylene oxide c.Halogens d.Salt 50 In order for a virus to replicate c Ananthanarayan and Paniker's D Unit III a.the capsid must enter the host cell cytoplasm Textbook of Microbiology, reprint, b.the host cell must be undergoing mitosis Orient Blackswan, 2006 c.the genome must be released in the cytoplasm d.the host cell must lack a cell membrane 51 Which of the following statements is not true of viruses? a Ananthanarayan and Paniker's D Unit III a.Viruses have been successfully grown in pure cultures in test tubes Textbook of Microbiology, reprint, b.All viruses are obligatory intracellular parasites Orient Blackswan, 2006 c.All viruses have either DNA or RNA as their genetic material d.Viruses probably arose from small fragments of cellular chromosomes 52 The size of viruses is usually measured in c Ananthanarayan and Paniker's E Unit III a.centimeters Textbook of Microbiology, reprint, b.micrometers Orient Blackswan, 2006 c.nanometers d.millimeters 53 When a virus enters a cell but does not replicate immediately, the situation is a Ananthanarayan and Paniker's MD Unit III called Textbook of Microbiology, reprint, a.lysogeny Orient Blackswan, 2006 b.fermentation c.symbiosis d.Synergism 54 The function of a viral capsid is d Ananthanarayan and Paniker's E Unit III a.protection against the viral genome from physical and enzymatic destruction Textbook of Microbiology, reprint, b.providing binding sites that enable the virus to attach to specific receptor sites Orient Blackswan, 2006 on the host cell c.serving as a vehicle of transmission from one host to another d.all of the above 55 Fungi that lack partitions (septa) are called b Ananthanarayan and Paniker's E Unit III a.ahyphae Textbook of Microbiology, reprint, b.coenocytic Orient Blackswan, 2006 c.yeast d.conidia 56 __________ exhibit yeast-like growth at human body temperatures and mold- a Ananthanarayan and Paniker's D Unit III like growth at room temperature. Textbook of Microbiology, reprint, a.Dimorphic fungi Orient Blackswan, 2006 b.Black bread molds c.Sac fungi d.Water molds 57 __________ produce motile sexual and asexual spores. d Ananthanarayan and Paniker's E Unit III A.Slime molds Textbook of Microbiology, reprint, b.Dimorphic fungi Orient Blackswan, 2006 c.Club fungi d.Water molds 58 Fungi d Ananthanarayan and Paniker's E Unit III a.are photosynthetic Textbook of Microbiology, reprint, b.are prokaryotic cells Orient Blackswan, 2006 c.have cell walls of peptidoglycan d.secrete extracellular enzymes to breakdown nutrients 59 Fungi in the division Deuteromycota are characterized by the fact that d Ananthanarayan and Paniker's D Unit III a.they only reproduce sexually Textbook of Microbiology, reprint, b.they form sexual spores called deuterospores Orient Blackswan, 2006 c.they are incapable of sexual reproduction d.a method of sexual reproduction has not been identified 60 __________ produce zygospores. d Ananthanarayan and Paniker's E Unit III a.Slime molds Textbook of Microbiology, reprint, b.Dimorphic fungi Orient Blackswan, 2006 c.Club fungi d.Black bread molds 61 The desire to maintain a safe laboratory environment for all begins with a Clinical Laboratory Science: The Basics E Unit IV _________? and Routine Techniques, a. prevention The Basics Series, c. microbiology Authors Jean Jorgenson Linné, Karen b. ubiquity Munson Ringsrud d. accidents Edition 4, illustrated, reprint 62 When a chemical splashes in the eye rinse for ________? d Clinical Laboratory Science: The Basics E Unit IV a. 10 seconds and Routine Techniques, c. 5 minutes The Basics Series, b. 30 seconds Authors Jean Jorgenson Linné, Karen d. 15 minutes Munson Ringsrud Edition 4, illustrated, reprint 63 Which of the following type of Personal Protective Equipment (PPE) is not d Clinical Laboratory Science: The Basics D Unit IV frequently used? and Routine Techniques, a. Safety glasses The Basics Series, d. Gloves Authors Jean Jorgenson Linné, Karen b. Lab Coats Munson Ringsrud d. Ointment Edition 4, illustrated, reprint 64 Chemical, reagents or broth cultures should not be pipetted by ___________? a Clinical Laboratory Science: The Basics MD Unit IV a. mouth and Routine Techniques, c. pipetter The Basics Series, b. Straw Authors Jean Jorgenson Linné, Karen d. rubber Munson Ringsrud Edition 4, illustrated, reprint 65 Good work practices include, c Clinical Laboratory Science: The Basics E Unit IV a. smelling and tasting chemicals and Routine Techniques, b. not washing hands before and after lab The Basics Series, c. confining long hair and loose clothing Authors Jean Jorgenson Linné, Karen d. using damaged equipment and glassware. Munson Ringsrud Edition 4, illustrated, reprint 66 What is the name of the procedure performed under sterile conditions to c Clinical Laboratory Science: The Basics D Unit IV eliminate contamination in hopes to obtain a pure culture of one type of and Routine Techniques, microorganism? The Basics Series, a. sterilization technique Authors Jean Jorgenson Linné, Karen c. disinfectant technique Munson Ringsrud b. aseptic technique Edition 4, illustrated, reprint 67 b.After pathogen technique a biohazard spill is covered with paper towels and disinfectant solution, it c Clinical Laboratory Science: The Basics E Unit IV must sit for _________ minutes? and Routine Techniques, a. 5 The Basics Series, c. 60 Authors Jean Jorgenson Linné, Karen b. 30 Munson Ringsrud d. 20 Edition 4, illustrated, reprint 68 ______________ is needed as a source of nutrient for the growth and d Clinical Laboratory Science: The Basics E Unit IV reproduction of microbes. and Routine Techniques, a. pathogens The Basics Series, c. reagents Authors Jean Jorgenson Linné, Karen b. bacteria Munson Ringsrud d. media Edition 4, illustrated, reprint 69 To prevent the contamination of microscopes and surrounding areas a Clinical Laboratory Science: The Basics E Unit IV disenfect/clean used slides, prepared by student, with and Routine Techniques, a. 70% ethanol and lens paper The Basics Series, c. acetone and lens paper Authors Jean Jorgenson Linné, Karen b. 5% methylene blue and lens paper Munson Ringsrud d. water and lens paper Edition 4, illustrated, reprint 70 Which type of antibiotics stop bacteria from growing without killing them? d Clinical Laboratory Science: The Basics E Unit IV a.Narrow Spectrum and Routine Techniques, b.Broad Spectrum The Basics Series, c.Bactericidal Authors Jean Jorgenson Linné, Karen d.Bacteriostatic Munson Ringsrud Edition 4, illustrated, reprint 71 one level assay of antibiotics is used to determine a Theory and application of E Unit IV a.Concentration Microbiological Assay b.Potency Author William Hewitt c.D value Publisher Elsevier, 2012 d.E value 72 Two level assay of antibiotics is used to determine b Theory and application of E Unit IV a.Concentration Microbiological Assay b.Potency Author William Hewitt c.D value Publisher Elsevier, 2012 d.E value 73 Zone of inhibion measured in microbiological assay of a Theory and application of E Unit IV a.Antibiotics Microbiological Assay b.Antobodies Author William Hewitt c.Vitamins Publisher Elsevier, 2012 d.Water 74 Zone of growth measured in microbiological assay of c Theory and application of E Unit IV a.Antibiotics Microbiological Assay b.Antobodies Author William Hewitt c.Vitamins Publisher Elsevier, 2012 d.Water 75 Cleanrooms maintain particulate-free air through the use of a Clean Room Technology in ART Clinics: D Unit IV a.HEPA A Practical Guide b.CEPA Editors Sandro C. Esteves, Alex C. c.AEPA Varghese, Kathryn C. Worrilow d.DEPA Edition illustrated Publisher CRC Press, 2016 76 Cleanrooms are classified by d Clean Room Technology in ART Clinics: MD Unit IV a.How clean the chemical is A Practical Guide b.How clean the water is Editors Sandro C. Esteves, Alex C. c.How clean the agar is Varghese, Kathryn C. Worrilow d.How clean the air is Edition illustrated Publisher CRC Press, 2016 77 A cleanroom is a controlled environment that has a low level of except a Clean Room Technology in ART Clinics: E Unit IV a.Insects A Practical Guide b.Dust Editors Sandro C. Esteves, Alex C. c.Chemical vapors Varghese, Kathryn C. Worrilow d.Airborne microbes Edition illustrated Publisher CRC Press, 2016 78 Following is the lowest level of cleanroom standards b Clean Room Technology in ART Clinics: D Unit IV a.ISO 5 A Practical Guide b.ISO 9 Editors Sandro C. Esteves, Alex C. c.ISO 18 Varghese, Kathryn C. Worrilow d.ISO 12 Edition illustrated Publisher CRC Press, 2016 79 Median concentration in microbial assay is a Theory and application of D Unit IV a.1 µg/ml Microbiological Assay b.2 µg/ml Author William Hewitt c.3µg/ml Publisher Elsevier, 2012 d.4 µg/ml 80 Disadvantage of Agar diffusion method except d Theory and application of E Unit IV a.Agar thickness Microbiological Assay b.Microbial population Author William Hewitt c.Solubility of the drug Publisher Elsevier, 2012 d.Dilution 81 Most spoilage bacteria grow at c Food Processing Technology: Principles E Unit V a.acidic pH and Practice b.alkaline pH Woodhead Publishing Series in Food c.neutral pH Science, Technology and Nutrition d.any of the pH Author P J Fellows Edition 3, reprint, revised Publisher Elsevier, 2009 82 Which of the following acid will have higher bacteriostatic effect at a given pH? a Food Processing Technology: Principles E Unit V a.Acetic acid and Practice b.Tartaric acid Woodhead Publishing Series in Food c.Citric acid Science, Technology and Nutrition d.Maleic acid Author P J Fellows Edition 3, reprint, revised 83 Bacteria which is present in raw or undercooked meat, eggs, sea food and b Publisher Food Elsevier, Processing 2009 Technology: Principles E Unit V unpasteurized milk is and Practice a.E.coli Woodhead Publishing Series in Food b.Salmonella Science, Technology and Nutrition c.Staphylococcus Author P J Fellows d.Cyano bacteria Edition 3, reprint, revised 84 Milk and curry left over can be turned into sour spoiled at c Publisher Food Elsevier, Processing 2009 Technology: Principles MD Unit V a.High temperature and Practice b.Very low temperature Woodhead Publishing Series in Food c.Room temperature Science, Technology and Nutrition d.Constant temperature Author P J Fellows Edition 3, reprint, revised 85 Spoilage in food because of microbial activity can be prevented or delayed by c Publisher Food Elsevier, Processing 2009 Technology: Principles E Unit V a. Increasing the entry of micro-organisms in food and Practice b. Physical removal of dust Woodhead Publishing Series in Food c. Hindering the activity of micro-organisms Science, Technology and Nutrition d. Cleaning the environment Author P J Fellows Edition 3, reprint, revised 86 The growth of aerobic food spoilage and pathogenic microorganisms can be c Publisher Food Elsevier, Processing 2009 Technology: Principles E Unit V suppressed by and Practice a. Adding surfactants Woodhead Publishing Series in Food b. Removing Nutrients from food Science, Technology and Nutrition c. Exhausting Author P J Fellows d. Adding antibodies Edition 3, reprint, revised 87 In spore forming bacteria maximum resistance occurs at pH d Publisher Food Elsevier, Processing 2009 Technology: Principles E Unit V a. 4 and Practice b. 5 Woodhead Publishing Series in Food c. 6 Science, Technology and Nutrition d. 7 Author P J Fellows Edition 3, reprint, revised Publisher Elsevier, 2009 88 Any change that renders food unfit for human consumption is called b Food Processing Technology: Principles E Unit V a. Processing and Practice b. Spoilage Woodhead Publishing Series in Food c. Deterioration Science, Technology and Nutrition d. Preservation Author P J Fellows Edition 3, reprint, revised 89 Food intoxication is the ingestion of a Publisher Food Elsevier, Processing 2009 Technology: Principles E Unit V a. Toxin produced by microorganism and Practice b. Toxin producing additives Woodhead Publishing Series in Food c. Chemical interaction Science, Technology and Nutrition d. Toxin produced by body Author P J Fellows Edition 3, reprint, revised 90 Clostridium Botulinum is a Publisher Food Elsevier, Processing 2009 Technology: Principles E Unit V a. Bacteria and Practice b. Mold Woodhead Publishing Series in Food c. Yeast Science, Technology and Nutrition d. Virus Author P J Fellows Edition 3, reprint, revised 91 Type of yeast used for alcoholic fermentation is a Publisher Food Elsevier, Processing 2009 Technology: Principles MD Unit V a. Saccharomyces Cerevisiae and Practice b. Streptococcus thermophillus Woodhead Publishing Series in Food c. Acetobacter acceti Science, Technology and Nutrition d. Clostridium botulinum Author P J Fellows Edition 3, reprint, revised 92 What is the concentration of CO2 required for culturing animal cells? b Publisher Culture ofElsevier, 2009 A Manual of Basic Animal Cells: E Unit V a.2-5% Technique and Specialized Applications b.1-10% Author R. Ian Freshney c.10-15% Edition 7, reprint d.15-20% Publisher John Wiley & Sons, 2015 93 What are the main constituents of culture for animal cell growth? a Culture of Animal Cells: A Manual of Basic E Unit V a.Glucose and Glutamine Technique and Specialized Applications b.Growth factors Author R. Ian Freshney c.Cytokines Edition 7, reprint d.Water Publisher John Wiley & Sons, 2015 94 The human fibroplast is a classical example of a Culture of Animal Cells: A Manual of Basic MD Unit V a.stable primary cell lines Technique and Specialized Applications b.established cell lines Author R. Ian Freshney c.cell transformations Edition 7, reprint d.none of these Publisher John Wiley & Sons, 2015 95 pH of culture medium is initially controlled by b Culture of Animal Cells: A Manual of Basic E Unit V a.presence of CO2 Technique and Specialized Applications b.presence of bicarbonate buffer Author R. Ian Freshney c.addition of bases Edition 7, reprint d.none of these Publisher John Wiley & Sons, 2015 96 In animal cell culture, particularly mammalian cell culture, transformation b Culture of Animal Cells: A Manual of Basic E Unit V means Technique and Specialized Applications a.uptake of new genetic material Author R. Ian Freshney b.phenotypic modifications of cells in culture Edition 7, reprint c.increased division of the cell Publisher John Wiley & Sons, 2015 d.release of genetic information 97 Cells which have undergone transformation frequently become a Culture of Animal Cells: A Manual of Basic MD Unit V a.anchorage independent Technique and Specialized Applications b.anchorage dependent Author R. Ian Freshney c.stable Edition 7, reprint d.unstable Publisher John Wiley & Sons, 2015 98 Which of the following is not a source of energy in active muscle cells? c Culture of Animal Cells: A Manual of Basic MD Unit V a.Creatine phosphate Technique and Specialized Applications b.ATP Author R. Ian Freshney c.Lactic acid Edition 7, reprint d.Glucose Publisher John Wiley & Sons, 2015 99 Sometimes cell lines can be cultured for such a long time that they apparently a Culture of Animal Cells: A Manual of Basic D Unit V develop the potential to be subcultured indefinitely in vitro. Such cells lines are Technique and Specialized Applications called Author R. Ian Freshney a.established cell lines Edition 7, reprint b.primary cell lines Publisher John Wiley & Sons, 2015 c.secondary cell lines d.propagated cell lines 100 Accumulation of lactate leads to c Culture of Animal Cells: A Manual of Basic MD Unit V a.increase in pH Technique and Specialized Applications b.no change in pH Author R. Ian Freshney c.reduction in the pH of culture hence loss of cell viability Edition 7, reprint d.no loss of cell viability Publisher John Wiley & Sons, 2015  Chapter title/content History of microbiology History of microbiology History of microbiology History of microbiology Microscopy History of microbiology Study of bacteria History of microbiology Microscopy Study of bacteria Study of bacteria Study of bacteria Study of bacteria Study of bacteria Growth curve Study of bacteria Study of bacteria Study of bacteria Study of bacteria Study of bacteria Study of bacteria Study of bacteria staining techniques staining techniques staining techniques staining techniques staining techniques staining techniques sterilization sterilization sterilization sterilization sterilization sterilization sterilization sterilization sterilization sterilization sterilization sterilization disinfectant disinfectant disinfectant disinfectant disinfectant  disinfectant disinfectant disinfectant disinfectant Study of Virus Study of Virus Study of Virus Study of Virus Study of Virus Study of Fungi Study of Fungi Study of Fungi  Study of Fungi Study of Fungi Study of Fungi Designing of aseptic area Designing of aseptic area Designing of aseptic area  Designing of aseptic area Designing of aseptic area Designing of aseptic area Assessment of a new antibiotic General aspects-environmental cleanliness General aspects-environmental cleanliness General aspects-environmental cleanliness Assessment of a new antibiotic Assessment of a new antibiotic Assessment of a new antibiotic Assessment of a new antibiotic General aspects-environmental cleanliness General aspects-environmental cleanliness General aspects-environmental cleanliness General aspects-environmental cleanliness Assessment of a new antibiotic Assessment of a new antibiotic Types of spoilage Types of spoilage spoilage spoilage spoilage spoilage spoilage  spoilage spoilage spoilage spoilage Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture Growth of animal cells in culture