Histopathology Stains PDF

HISTOPATHOLOGY: Stains Stains Purposes Hematoxylin Routine histologic studies Aluminum hematoxylin Used for progressive or regressive staining, blueing Ehrlich’s hematoxylin...

HISTOPATHOLOGY: Stains Stains Purposes Hematoxylin Routine histologic studies Aluminum hematoxylin Used for progressive or regressive staining, blueing Ehrlich’s hematoxylin Used for regressive staining Cole’s hematoxylin Routine purposes, in sequence with Celestine blue Mayer’s hematoxylin Used for regressive or progressive staining Iron hematoxylin Used for regressive staining Regaud’s hematoxylin Used for mitochondria by light microscopy Weigert’s hematoxylin For muscle fibers and connective tissues, Entamoeba histolytica Heidenhains hematoxylin For mitosis study, regressive staining, chromatin chromosomes, nucleoli, mitochondria Phosphotungstic acid hematoxylin Nuclei, muscles striations, fibrin (blue); collagen, bone, cartilage (orange-red) Eosin For cytoplasm Van Gieson’s stain (Acid Fuchsin-Picric Connective tissue acid) Masson stain (Acid fuchsin) Collagen, smooth muscle, mitochondria permeability Acridine orange DNA – green fluorescence, RNA – red fluorescence Acridine red 3B Deposits of Calcium salts Alcian blue Mucopolysaccharides Red- Calcium > Alizarin red s Calcium Aniline blue Epithelial sections Azocarmine Nuclei – deep red, cytoplasm – pale red Basic fuchsin Deep staining of acid fast organisms Benzidine Hemoglobin Bismarck brown Diphtheria organisms Carmine For fresh materials Celestine blue Alternative for iron hematoxylin Congo red Amyloid, embryo Cresyl violet Nervous tissues, nissl bodies Ethidium bromide DNA Geimsa stain Blood pathogens Leukocytes Gold sublimate Used for MI Gram’s iodine Bacteria Janus green B Mitochondria Malachite green Erythrocyte Masson’s trichrome Keratin, muscle fibers (red), collagen, bone (green or blue), cytoplasm (light red or purple) Methyl green Chromatin Methyl blue Substitute to janus green b Nile red Lipid globules Oil red o Neutral lipids Hepatitis B Orcein Elastic fibers 1 of 1  Osmium tetroxide Myelin PAS Glycogen, mucin, glycoprotein Phosphotungstic acid viruses Picric acid Connective tissue Prussian blue O Iron -Rhodamine B Glandular tissues Blood Safranin Red – nuclei, collagen – yellow Silver nitrate Spirochetes, reticulum, fiber strains Toluidine blue Nissl granules Van Gieson Stain Collagen, elastic fibers Victoria blue Neuroglia Von Kossa stain Phosphate and carbonates Wright’s stain blood Hematoxylin is classified according to mordant used: I. Alum hematoxylin  Stain-Ripening agent:  Cole’s – alcoholic iodine  Ehrlich’s – natural  Mayer’s – sodium iodate  Carrazi’s – potassium iodate  Delafields – natural  Gill’s – sodium idoate II. Iron Hematoxylin  Weigert’s – natural  Heidenhain’s – natural  Verhoeff’s – natural  Loyez – natural III. Tungsten hematoxylin  Mallory phosphotungstic acid hemotxylin – natural IV. Molybdic acid hematoxylin  Thomas – hydrogen peroxide V. Lead hematoxylin  Social – no ripening VI. No mordant hematoxylin  Mallory hematoxylin – no ripening 2 of 2 Carbohydrates > - PAS /Periodic Acid Schiff) 1-2 Glycols -.