CLINICAL CHEMISTRY final.pptx

EAC Medical Center - Cavite Clinical Chemistry Joselee Jean Rosemary R. Cablaida, RMT Clinical Chemistry Section Head Objectives After this lecture, students will be able to Describe each equipment used in Clinical Chemistry Section. Operate the machines with the supervision o...

EAC Medical Center - Cavite Clinical Chemistry Joselee Jean Rosemary R. Cablaida, RMT Clinical Chemistry Section Head Objectives After this lecture, students will be able to Describe each equipment used in Clinical Chemistry Section. Operate the machines with the supervision of staff in charge. Enumerate the tests done for each machine. CLINICAL CHEMISTRY ⮚ Refers to the biochemichal analysis of body fluids, in support of the diagnosis and treatment of disease. ⮚ Utilizes chemical reactions to identify or quantify levels of chemical compounds in bodily fluids. EACmed CC Section 1. Routine Blood Chemistry ⮚ Fasting/ Random Blood Sugar, BUN, Creatinine, Uric Acid, ALT, AST, Lipid Profile (Total Cholesterol, Triglyceride, HDL, LDL) 2. Special Blood Chemistry ⮚ Total Protein, Albumin, Alkaline Phosphatase, Lipase, Amylase, Total/ Direct/ Indirect bilirubin, Lactate Dehydrogenase. CLINICAL CHEMISTRY 3. Electrolytes ⮚ Sodium, Potassium, Chloride, Calcium, Phosphorus, Magnesium 4. Other tests ⮚ Glycosylated hemoglobin (HbA1c) ⮚ Cardiac markers (CKMB, Troponin-I, Myoglobin) Analyzers 1. Mindray BS-430 Chemistry Analyzer 2. Cobas c111 Chemistry Analyzer 3. Easylyte Plus Na/K/ Cl Analyzer 4. Easylyte Expand Na/K/ Cl/ Ca Analyzer 5. Finecare FIA Meter 6. GP Getein Biotech Mindray BS-430 ✔ Fully-Automated Chemistry Analyzer ✔ Principle: Colorimetry, Turbidimetry, ISE ✔ Throughput for biochemistries: Up to 420 tests/hour ✔ Throughput for biochemistries and ISE: 626 tests/ hour ✔ Sample volume:1.5 ul - 45ul Mindray BS-430 Parts 1 2 4 3 7 5 6 Operating the Analyzer Operating the Analyzer ✔ Running of Samples 1. Checks before Startup Water supply Operating the Analyzer ✔ Running of Samples 1. Checks before Startup Power supply Operating the Analyzer ✔ Running of Samples 1. Checks before Startup Printer Operating the Analyzer ✔ Running of Samples 1. Checks before Startup Waste connection Operating the Analyzer ✔ Running of Samples 1. Checks before Startup Probes and Mixers Operating the Analyzer ✔ Running of Samples 2. Startup Operating the Analyzer ✔ Running of Samples 2. Programming and Processing Operating the Analyzer ✔ Running of Samples 2. Programming and Processing Operating the Analyzer ✔ Running of Samples 2. Programming and Processing Operating the Analyzer ✔ Running of Samples 2. Programming and Processing Operating the Analyzer ✔ Running of Samples 2. Programming and Processing Operating the Analyzer ✔ Performing Calibration Operating the Analyzer ✔ Performing Calibration Operating the Analyzer ✔ Performing Calibration Operating the Analyzer ✔ Performing Quality Control Operating the Analyzer ✔ Performing Quality Control Operating the Analyzer ✔ Performing Quality Control Validating Test Results Operating the Analyzer ✔ Loading Reagents Operating the Analyzer ✔ Loading Reagents Analytes Glucose ⮚ Carbohydrate metabolism disorders, Diabetes mellitus, hypoglycemia, Insulinoma ⮚ Method: Glucose oxidase - peroxidase method ⮚ Turn around time: 2 hours ⮚ Linearity: 0.3 - 25 mmol/L ⮚ Normal range: 3.9 - 6.4mmol/L Analytes Cholesterol ⮚ Atherosclerosis, Effect of drugs or low-fat diet ⮚ Method: Cholesterol oxidase- Peroxidase ⮚ Turn around time: 2 hours ⮚ Linearity: 0.1-20 mmol/L ⮚ Normal range: