Turbidimetry Lab 5 PDF

Advanced laboratory technique Lab/5 Turbidimetry MSc.Yasmeen Kateb  Introduction Turbidimetry is an analytical technique used to measure the turbidity (cloudiness) of a fluid due to the presence of suspended particles , the method is comm...

Advanced laboratory technique Lab/5 Turbidimetry MSc.Yasmeen Kateb  Introduction Turbidimetry is an analytical technique used to measure the turbidity (cloudiness) of a fluid due to the presence of suspended particles , the method is commonly used to quantify antigen-antibody complexes, the formation of antigen-antibody complexes increases the turbidity of the sample, which is measured by observing the transmission level of visible light.  Principle Light Scattering: When light passes through a sample containing suspended particles, the particles scatter the light, the amount of light that reaches the detector is reduced, and this reduction is measured to determine the turbidity.  Applications ❑Water quality: Monitoring water treatment processes and detecting contaminants. ❑Biology: Measuring cell concentrations in bacterial cultures or other biological suspensions. ❑Chemical Analysis: Assessing the purity of solutions by detecting particulate matter. ❑Pharmaceuticals: Ensuring the clarity and stability of pharmaceutical products.  Advantages and disadvantages Advantages: Simplicity: The technique is straightforward and easy to perform. Speed: Results can be obtained quickly. Non-Destructive: It does not alter the sample during measurement. Disadvantages: Interference: The presence of colored substances can interfere with the measurement. Limitation on Sample Type: Not suitable for all types of samples, especially those with very low or very high turbidity.