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**[Questions]** 1. Define Respiration. Respiration is the breakdown of glucose to release energy. 2. Distinguish between anaerobic and aerobic respiration. Anaerobic respiration does not require oxygen. Aerobic respiration requires oxygen. 3. Write a balanced word equation for respiration....

**[Questions]** 1. Define Respiration. Respiration is the breakdown of glucose to release energy. 2. Distinguish between anaerobic and aerobic respiration. Anaerobic respiration does not require oxygen. Aerobic respiration requires oxygen. 3. Write a balanced word equation for respiration. Glucose+Oxygen=Carbon Dioxide+Water + Energy 4. Write a balanced chemical equation for respiration. C6H12O6+ 6O2= 6CO2+ 6H2O 5. What is the name of the first stage of respiration? Glycolysis 6. Where in the cell does glycolysis take place and why? In the cytosol due to presence of the necessary enzymes. 7. Name the three-carbon molecule formed at the end of glycolysis. Pyruvic acid 8. Where does stage two take place? The mitochondrion. 9. In stage two, what is pyruvic acid broken down into? Acetyl Co-A. 10. Acetyl Co-A enters a series of reactions. Name this series of reactions. Krebs cycle. 11. What are the three products of the Krebs cycle? NADH, ATP, CO2 12. What is pyruvic acid broken down into in anaerobic respiration in yeast and plants? Ethanol and CO2 13. What is pyruvic acid broken down into in anaerobic respiration in some bacteria and animal cells? Lactic acid. 14. What is phosphorylation? The addition of a phosphate group. ADP+P to give ATP, and H2) as a by-product. **[Experiment questions]** 1. What is the optimum temperature for this fermentation process. 30 degrees Celsius. 2. How did you maintain this temperature at a constant temperature for the duration of the experiment? By use of a water bath. 3. Why did you boil the glucose solution? To remove any oxygen present. 4. What was the purpose of the layer of oil? To prevent oxygen and other gases from re-entering the glucose solution.  5. How did you know the fermentation had finished. No more bubbles of CO2 were being produced. 6. What is the name of the chemical test for the presence of alcohol? The Iodoform test.  7. What chemicals are used in the Iodoform test? Potassium iodide and sodium hypochlorite.  8. What is a positive result for alcohol? Pale yellow crystals are formed. 

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