Research Strategies PDF
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Xiamen University
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Summary
This document provides an overview of different research strategies, focusing on various microscopy techniques such as electron microscopy, optical microscopy, and fluorescence microscopy. It also covers topics like histology, cell culture, and molecular biology techniques. The document contains questions for the reader to answer.
Full Transcript
Research strategies Friday 11 October 2024 10:06 AM 1. Electron microscope 2. Histology 3. Microscope 4. Cell culture 5. Flow cytometry 6. Isolation of genes and cDNAs 7. Protein purification 8. Organelle purification 9. Protein identification...
Research strategies Friday 11 October 2024 10:06 AM 1. Electron microscope 2. Histology 3. Microscope 4. Cell culture 5. Flow cytometry 6. Isolation of genes and cDNAs 7. Protein purification 8. Organelle purification 9. Protein identification Optical microscope Uses visible light and systems of lens to magnify small objects Two techniques Used to enchant the visuality of transparent or unstained biological specimens Shows the differences in refractive index between the specimen and the medium Powerful technique Uses visible light to excite fluorescent molecules within the specimen Causing them to emit light of different wavelength This light can then be detected and visualised revealing structures within the specimen. Fluorescent Bright field Phase contrast Specific excitation wavelength Entire field of view Entire field of view Illumination Based on fluorescent emission Based on absorption of light Based on phase differences in light Contrast Fluorescently labeled Stained specimen Unstained or transparent specimen Suitablity Requires fluorescent probes Limited to strained specimens May introduce halos Limits Can be complex Powerful technique Used to visualise extremely small objects Employs a beam of electrons illuminate the specimen Allows for higher resolution and magnification Study of tissues Molecular biology technique Used to detect and localize specific rna and dna sequences within cells and tissues Grow cells in labs WHAT IS THE MAIN REASON FOR USING A MICROSCOPE To see small objects that can’t be seen with the naked eye WHAT IS PCR Amplify dna meaning it makes millions of copies of a specific dna sequence from a small amount of startling material HOW TO ISOLATE PROTEIN By breaking cells,separating and purifying the protein HOW TO IDENTIFY PROTEIN Use mass spec or antibody
