HEMA PDF

HEMA 1. 2. 3. 4. 5. 6. 7. Most critical step in phlebotomy - Patient ID Ascorbate cyanide test is used in - G6PD a. Most common enzymopathy in EMP - PK def b. Most common enzymopathy in Pentose phosphate pathway - G6PD c. Pernicious anemia - Vit B12 def; Schilling test i. Administer Cobalt57-labeled...

HEMA 1. 2. 3. 4. 5. 6. 7. Most critical step in phlebotomy - Patient ID Ascorbate cyanide test is used in - G6PD a. Most common enzymopathy in EMP - PK def b. Most common enzymopathy in Pentose phosphate pathway - G6PD c. Pernicious anemia - Vit B12 def; Schilling test i. Administer Cobalt57-labeled cobalamin (vit. B12) orally in the ileum ii. Overnight fasting, 24-hr urine 8. Pattern @37 ℃ for 48 hrs +Glucose +ATP condition Type I hemolysis Corrected Not corrected G6PD Type II Hemolysis Not corrected Corrected PK def 9. Type III Hemolysis Hereditary Spherocytosis, triose phosphate isomerase def 10. Corrected Order of draw- Sterile, light blue, red, gold, green, lavender, grey Leukemoid reaction - >50.0 x 10e9/L with left shift EDTA - chelates Ca2+ Lipidoses a. Gaucher’s cell- wrinkled, onion skin-like b. Tay sachs cell- with vacuoles c. Niemann-Pick cell- foamy cytoplasm d. Sea blue histiocytes - blue-green cytoplasm (Wright’s) *Gaucher’s - b-glucosidase deficiency *Tay sachs - ganglioside * Niemann-Pick - sphingomyelinase def Angle between slides for blood smearing: 30-45° (30-40° sa book) a. >40° or fast = short, thick smear b. 15% a. MDS-SLD/-MLD: BM blasts 160/100 mmHg (hypertension) - never b.