Practical Session 3 PDF

Practical Session 3 Dr. Gehane Hamed Prof. of Physiology  Erythrocyte Sedimentation Rate (ESR)  Erythrocyte Sedimentation Rate (ESR) This picture shows a rack holding Westergreen Red blood cells have tubes, in which settled, leaving plasma at anticoagulated...

Practical Session 3 Dr. Gehane Hamed Prof. of Physiology  Erythrocyte Sedimentation Rate (ESR)  Erythrocyte Sedimentation Rate (ESR) This picture shows a rack holding Westergreen Red blood cells have tubes, in which settled, leaving plasma at anticoagulated whole the top of the tube. blood has just been added. Reading: 18 mm/hour Time: 0 Time: one hour 1- This tube is called … Westergreen tubes …. 2- It is used in estimation of …ESR… 3- The normal value of this test is …4-6 mm/hr…up to 12… in males and …6-10 mm/hr… up to 20 in females.4-It increases physiologically in –menstruation, pregnancy, exercise & pathologically in –infection, trauma, tumour---------- 1- This tube is called ……Westergreen tube…. 2- It is used in estimation of ……ESR…… 3- The reading of the tube is … ---mm/hr… 4- The RBCs aggregate forming …Rouleaux…which increase their density allowing them to sediment. -What is the value ------- -Compared to normal values is it normal or not What increases it? Is it diagnostic ?? Not diagnostic , Prognostic only Name the anticoagulant used in this test? 4 cc of venous blood + 1 cc of 3.8% Na citrate  RBCs Osmotic Fragility Test - It tests the ability of RBCs to resist hemolysis when challenged with hypotonic solutions. - It is dependant on their surface area to volume ratio (S/V).  RBCs Osmotic Fragility Test - S/V ratio, the more resistant the RBCs to hemolysis and the less fragile. S/V +++ S/V + Resistance Resistance Fragility Fragility Normal Spherocytosis Name of Test----------------- Partial or complete hemolysis ?? Normal value (0.45%) ?? Type of solution ?? How does it cause hemolysis? Reason for resistance of RBCs to hemolysis? Used for diagnosis of ………? Hemolysis starts at …?? Name of Test----------------- Partial or complete hemolysis ?? Normal value (0.45%) ?? Type of solution ?? How does it cause hemolysis? Reason for resistance of RBCs to hemolysis? Used for diagnosis of ………? Hemolysis starts at …??  1 2 3 1- This test is called …Osmotic fragility test…. 2- Tube “1” shows ……no haemolysis…, tube “2” shows …partial haemolysis… And tube “3: shows …complete haemolysis.  3 2 1 3 2 1 1- This test is called ………………………. 2- Tube “1” shows ………………………………, tube “2” shows ………… And tube “3: shows …………  4 1 1 2 3 1- This test is called ………………………. 2- Tube “1” shows ………………………………while tube “4” shows ………………………….  A B 1- This test is called …Osmotic fragility test…. 2- In figure “A”: hemolysis starts at …0.58%.. and completed at …0.38%… 3- In figure “B”: hemolysis starts at …0.85%..and completed at …0.58%… 4- The disease that causes figure “B” is ……Hereditary Spherocytosis… -Mention the name of the test…………………. - Mention one use to such test……………………………………… -Comment on the test tube to which the arrow is pointing………