Genetics Study Guide PDF
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Uploaded by CourteousAntigorite2433
Wayne State University
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Summary
This document provides simplified answers to problems in genetics and inheritance, including definitions and examples of key terms. The guide also covers techniques like Punnett squares, mitosis, meiosis and gel electrophoresis.
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Here are the simplified answers based on the content provided: 1\. Define, give examples, and solve problems using genetics and inheritance terms. Definitions: Learn key terms like gene, allele, genotype, phenotype, homozygous, heterozygous, dominant, and recessive. Example: A genotype is the...
Here are the simplified answers based on the content provided: 1\. Define, give examples, and solve problems using genetics and inheritance terms. Definitions: Learn key terms like gene, allele, genotype, phenotype, homozygous, heterozygous, dominant, and recessive. Example: A genotype is the genetic makeup (e.g., Bb), and a phenotype is the physical trait (e.g., brown eyes). How to Solve: Use Punnett squares to predict offspring outcomes. 2\. Compare mitosis and meiosis. Mitosis: Produces 2 identical diploid cells (body cells); no genetic variation. Meiosis: Produces 4 non-identical haploid cells (gametes); increases genetic diversity through crossing-over. 3\. Solve mono- and dihybrid crosses for genotypic and phenotypic ratios. Monohybrid Cross: Focus on one trait (e.g., Tt x Tt → 3:1 ratio for phenotype, 1:2:1 for genotype). Dihybrid Cross: Focus on two traits (e.g., AaBb x AaBb → 9:3:3:1 phenotypic ratio). 4\. Determine genotypes/phenotypes of individuals from pedigrees. Use a pedigree chart to: Identify dominant or recessive traits. Determine if an individual is homozygous or heterozygous. 5\. Describe blood type compatibility and inheritance. Blood Types: A, B, AB, O. Compatibility: O is universal donor, AB is universal recipient. Inheritance: Based on antigens (A/B) and alleles (IA, IB, i). 6\. Principles of gel electrophoresis. How it works: DNA moves through a gel toward the positive end because it's negatively charged. Size separation: Smaller fragments move faster and farther. 7\. Describe the process of gel electrophoresis and staining. Steps: 1\. Load DNA samples into the gel. 2\. Run the gel with an electric current. 3\. Stain the gel to see the DNA bands. 8\. Applications of PCR and electrophoresis. PCR: Diagnosing diseases, identifying genetic mutations, forensic DNA matching. Electrophoresis: DNA fingerprinting, analyzing DNA size, checking PCR results. 9\. Interpret an example gel to determine traits. Look at the bands: Match band positions to a size marker. Compare bands to determine presence or absence of a gene/trait. Let me know if you need further clarification!