DNA Replication PDF

Memo No. Date Mo Tu Wo Th Fr S; Su DNA Replication. Replication process starts by separation of DNA strand in several different locations. these local tions are search in A and T bases. These are called "points of origin" or "Consensus areas." Bacterial DNA has only one point of origin. The...

Memo No. Date Mo Tu Wo Th Fr S; Su DNA Replication. Replication process starts by separation of DNA strand in several different locations. these local tions are search in A and T bases. These are called "points of origin" or "Consensus areas." Bacterial DNA has only one point of origin. The Enzyme that initially separates DNA strands in the point of origin is called DNA-A protein. Once this area is open (unzipped) it's called a replication bubble. To stabilize replication bubble and prevent DNA strands from reannealing, single Strand binding proteins (SSB proteins) bind to DNA strands. SSB proteins also protect single strends af DNA from being chopped up by endonualeases To further unwind the DNA spiral, special enzyme called Helicaze enters each replication fork site and pushes ahead separating DNA strand down- stream. Helicare requires A LOT of energy to break multiple hydrogen Bonds. As Helicase moves along and unzips DNA strends, it causes supercoils-tightly twisted zoves of DNA downstream. To resolve these supercoils we use a double function enzyme