Sedimentation and Centrifugation PDF

Summary

This document contains lecture notes on sedimentation and centrifugation, specifically focusing on Svedberg units and methods to overcome diffusion. It also covers sedimentation of blood and different types of separation.

Full Transcript

Sedimentation and Centrifugation: Svedberg units and Overcoming Diffusion Sedimentation of blood If blood that is treated with either Sodium Citrate or EDTA to prevent coagulation, it spontaneously separates into 3 visually distinct components (top- bottom) 1. Plasma (30% volume) 2. WBC 3....

Sedimentation and Centrifugation: Svedberg units and Overcoming Diffusion Sedimentation of blood If blood that is treated with either Sodium Citrate or EDTA to prevent coagulation, it spontaneously separates into 3 visually distinct components (top- bottom) 1. Plasma (30% volume) 2. WBC 3. RBC

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