Microbiology Diagrams PDF

Microscopy  Important Vocabulary : magnification n 1. apparent enlargement of an object 2. the ratio of image size to actual size A magnification of "100x" means that the image is 100 times bigger than the actual object. resolution n 1. clarity, sharpness 2. the ability of a microscope...

Microscopy  Important Vocabulary : magnification n 1. apparent enlargement of an object 2. the ratio of image size to actual size A magnification of "100x" means that the image is 100 times bigger than the actual object. resolution n 1. clarity, sharpness 2. the ability of a microscope to show two very close points separately  Magnification ocular power = 10x low power objective = 4x Medium power objective=10X high power objective = 40X a) What is the highest magnification you could get using this microscope ? 400x Ocular x high power = 10 x 40 = 400. (We can only use 2 lenses at a time, not all three.) Carrying a Microscope  Steps to Use: 1. Rotate the low power objective into place and make sure the stage is all the way down. 2. Place slide on stage making sure object to be viewed is centered over the hole in the stage. Use the stage clips to hold the slide in place. 3. Turn light on. 4. Focus first with the coarse adjustment knob. Once in focus on low power, turn the nosepiece until the next higher lens is in place. 5. Use FINE adjustment knob ONLY and focus the object.  Remember: 1. If you are seeing perfectly round, clear circles then you just may be looking at air bubbles. Check your slide and try again. 2. Microscopes must always be properly put away. 3. Slides and cover-slips should be washed, dried, and returned to their proper place. Biological Diagrams  Before you begin…. Ensure you have blank white paper Ruler, pencil and eraser Use a Petri dish for your field of View (FOV) and draw the biological diagram inside  Plan the composition of your drawing Use the left and centre two-thirds of the page for drawing. Leave space for labels on the right side of the drawing. Only two diagrams per page  Drawing your Diagram Draw in pencil, label in ink (or pencil is fine) Draw only what you see. Draw simple two-dimensional images Do not sketch. Draw firm, clear lines.  DO NOT shade or colour! Stippling (a series of dots) may be used to indicate a darker area. You can draw lines for outlines of structures. Use double lines to indicate thick structures. Include labels, a title, magnification and size  Labeling your Drawing Lower case letters should be used to label names of structures. Do not print labels directly on the drawing. Print your labels in an even column on the right side of the drawing. Use a ruler to draw label lines. Label lines should be horizontal and drawn from the structure being identified to the label.  Labeling your Drawing Lower case letters should be used to label names of structures. Do not print labels directly on the drawing. Print your labels in an even column on the right side of the drawing. Use a ruler to draw label lines. Label lines should be horizontal and drawn from the structure being identified to the label.  No arrows on your lines Labels should be at the end of the line Magnification and size of stuctures/cells should be at the bottom right of the diagram Mag is shown as 40X or 100X or 400X Diameter of Field of View (FOV ∙ At 40x magnification you will be able to see 5mm or 5000 micrometres (μm) ∙ At 100x magnification you will be able to see 2mm or 2000 micrometres (μm) ∙ At 400x magnification you will be able to see 0.45mm, or 450 micrometres (μm) ∙ Write all sizes in μm Estimating size: Estimate how many times the cell you want the size of will fit across the FOV and divide it by the appropriate amount of micrometres for that magnification  The FOV above is 2mm or 2000μm The cell fits about 4 times across 2000 = 500μm= size of 1 cell 4 Biological diagram