Media Preparation and Sterilization PDF

Media Preparation and Sterilization Objectives Learn the proper way of preparing culture media Be able to practice aseptic technique when performing all procedures in a microbiology laboratory Know the basic importance of isolating bacteria Learn the basic techniques in the cultivation an...

Media Preparation and Sterilization Objectives Learn the proper way of preparing culture media Be able to practice aseptic technique when performing all procedures in a microbiology laboratory Know the basic importance of isolating bacteria Learn the basic techniques in the cultivation and isolation of microorganisms. Key words Agar Sterilization Aseptic techniques Inoculum Five I’s (Inoculation, Incubation, Isolation, Inspection & Identification) Colony (bacterial characteristics) Culture Properties of Media Forms of Culture Cont. Types of Culture Media Types of Culture Media. Types of Culture Media MAC, Salmnoella (left), E. coli (right) Common Media Neisseria gonorrhea Cont. Part 2: Cultivation of Bacteria LABORATORY NEXT WEEK - HANDS ON Macroscopic examination BACTERIAL COLONIES Thank you! Next meeting – quiz and hands-on activity Next discussion - Topic – staining