MBI DPP-4 (1) PDF - Molecular Basis of Inheritance

Molecular Basis of Inheritance TOPIC – Translation , Lac operon, Human Genome Project, DNA Fingerprinting 1. Which of following is sign as 3. Activation of amino acids during beginning of translation? translation is done by: (1) When the large subunit of protein...

Molecular Basis of Inheritance TOPIC – Translation , Lac operon, Human Genome Project, DNA Fingerprinting 1. Which of following is sign as 3. Activation of amino acids during beginning of translation? translation is done by: (1) When the large subunit of protein (1) RNA polymerase factory of cell encounters an mRNA. (2) Methionine (2) When the small subunit of protein (3) Initiation factor factory of cell encounters an mRNA. (4) Amino acyl tRNA synthetase (3) When the small subunit of protein factory of cell encounter a tRNA. 4. Amino acids are activated in the presence of ATP and linked to their (4) When the large subunit of protein cognate tRNA. This process is called factory of cell encounters a tRNA. (1) charging of tRNA 2. Given below are two statements: (2) aminoacylation of tRNA Assertion (A) : If two charged tRNAs are brought close enough, the formation (3) charging of amino acid of peptide bond between them would be (4) Both 1 and 2 favoured energetically. 5. Given below are two statements: Reason (R) : The presence of a catalyst would reduce the rate of peptide bond Assertion (A) : Regulation of an operon by formation. repressor is referred to as negative regulation. In the light of the above statements, choose the most appropriate answer Reason (R) : Operon under control of from the options given below positive regulation are regulated by activators. (1) Both Assertion (A) and Reason (R) are true and Reason (R) is a correct Choose the most appropriate answer from explanation of Assertion (A). the options given below: (2) Both Assertion (A) and Reason (R) are (1) Both (A) & (R) are correct but (R) is true but Reason (R) is not a correct not the correct explanation of (A). explanation of Assertion (A). (2) (A) is correct but (R) is not correct. (3) Assertion (A) is true and Reason (R) is (3) (A) is not correct but (R) is correct. false. (4) Both (A) & (R) are correct and (R) is (4) Assertion (A) and Reason (R) both are the correct explanation of (A). false. Seep Pahuja Biology-Official seep.pahuja 6. Identify the enzymes of Lac operon 9. Given below are two statements: labelled as A, B and C Statement-I : In lac operon, a polycistronic structural gene is regulated by a common promoter and regulatory genes. Statement-II: Such arrangement is very common in bacteria and is referred to as operon. (1) A-Permease , B-Transacetylase, C-- galactosidase In the light of the above statements, choose the most appropriate answer from (2) A-Transacetylase, B-Permease, C-- the options given below: galactosidase (1) Both statement I and II are correct. (3) A--galactosidase, B-Permease, C- Transacetylase (2) Statement I is correct but statement II is incorrect. (4) A--galactosidase, B-Transacetylase, C- Permease (3) Statement I is incorrect but statement II is correct. 7. Match the columns and select the correct option. (4) Both statement I and II are incorrect. Column I Column II 10. Given below are two statements; one is labelled as Assertion (A) and the other is (a) 2968 genes (i) Largest gene labelled as Reason(R). (b) 231 genes (ii) Total no. of genes Assertion (A) : In repetitive sequences a in human small stretch of DNA is repeated many (c) 2.4 million bases (iii) On chromosome 1 time. (d) 30,000 genes (iv) On chromosome Y Reason (R) : The bulk DNA forms a major peak and the other small peak are (1) a - iv, b - iii, c - i, d - ii referred as satellite DNA during (2) a - iii, b - iv, c - i, d - ii centrifugation. (3) a - iii, b - ii, c - iv, d - i In the light of the above statements, choose the correct answer from the (4) a - ii, b - iii, c - iv, d - i options given below : 8. Repressor mRNA will be formed in (1) Both (A) & (R) are correct but (R) is not (1) absence of inducer the correct explanation of (A). (2) presence of inducer (2) (A) is correct but (R) is not correct. (3) both 1 and 2 (3) (A) is not correct but (R) is correct. (4) presence of lac mRNA (4) Both (A) & (R) are correct and (R) is the correct explanation of (A). Seep Pahuja Biology-Official seep.pahuja 11. Find out the correct statement w.r.t. 14. In genetic fingerprinting, probe refers diagram representing translation in (1) a radioactively labelled ds RNA bacteria (2) a radioactively labelled ds DNA (3) a radioactively labelled ss DNA (4) a radioactively labelled ss RNA 15. Given below are two statements: Statement-I : Polymorphism is the basis (1) Last peptide bond formation takes of DNA fingerprinting. place between glycine and leucine. Statement-II : DNA polymorphism is (2) Ribosome moves from codon to codon inheritable. along the mRNA Choose the most appropriate answer (3) First amino acid is proline present at A- site (1) Both statement I and II are correct. (4) Last codon of m-RNA is CUU (2) Statement I is correct but statement II is incorrect. 12. In the lac operon, the genes a, i, y, and z code respectively for? (3) Statement I is incorrect but statement II is correct. (1) Repressor protein, permease, β- galactosidase, transacetylase. (4) Both statement I and II are incorrect. (2) Transacetylase, permease, β- 16. The main aim of the human genome galactosidase, repressor protein. project is: (3) β-galactosidase, transacetylase, (1) to introduce new genes into humans. repressor protein, permease. (2) to identify and sequence all the genes (4) Transacetylase, repressor protein, present in human DNA. Permease,β-galactosidase. (3) to develop better techniques for 13. What is the criterion for DNA comparing two different human DNA fragments movement on agarose gel samples. during gel electrophoresis? (4) to remove disease-causing genes from (1) The smaller the fragment size, the human DNA. farther it moves 17. In human genome largest gene is for: (2) Positively charged fragments move to (1) TDF farther end (2) Haemoglobin (3) Negatively charged fragments do not move (3) GLUT-4 (4) The larger the fragment size, the farther (4) Dystrophin it moves Seep Pahuja Biology-Official seep.pahuja 18. The probe used initially by Alec (4) Improve tools for data analysis. Jeffreys during development of DNA 20. Read the following statements: fingerprinting was; (A) Galactose may bind with the repressor (1) Ribozyme and inactive it (2) Macrosatellite (B) In the absence of lactose, the repressor (3) VNTR binds at the operator region (4) rDNA (C) The z-gene codes for permease 19. Identify the incorrect option (D) This was elucidated by Jacob and regarding goals of HGP. Monod (1) Determine the sequences of the 3 The correct statements w.r.t. lac operon million chemical base pairs that make up are human DNA. (1) A and B (2) Identify all the approximately 20,000- (2) A and C 25,000 genes in human DNA. (3) B and D (3) Store the information in databases. (4) A and D. ANSWER KEY 1. (2) 2. (3) 3. (4) 4. (4) 5. (1) 6. (3) 7. (2) 8. (3) 9. (1) 10. (1) 11. (2) 12. (4) 13. (1) 14. (3) 15. (1) 16. (2) 17. (4) 18. (3) 19. (1) 20. (3) Seep Pahuja Biology-Official seep.pahuja