Histopathology Techniques Reception & Selection

histopathology techniques reception & selection M.Sc: Eman Mahjoub Dr Eman mahgoub Specimen reception Purpose of reception step is to have a criteria for reception, registration and handling of specimen....

histopathology techniques reception & selection M.Sc: Eman Mahjoub Dr Eman mahgoub Specimen reception Purpose of reception step is to have a criteria for reception, registration and handling of specimen. Require a separate room with good ventilation and light.  Specimen must be delivered to the lab immediately after collection and kept in proper fixative Dr Eman mahgoub A separate room is required for specimen reception which acts as the interface between non-laboratory hospital staff, other visitors and the pathological laboratory. The area must be equipped with appropriate easily cleaned benching, adequate lighting, good ventilation, safety equipment, disinfectants and protective clothing. In the event of specimen spillage, e.g. body fluids, fixative leakage. Dr Eman mahgoub Specimen requirement : 1- Request form: Must be labeled with correct label and correct patient information as: Patient name, age, date of birth, sex…. Clinical history of patient. Date and time of collection. Type of specimen. The requesting physician name. Dr Eman mahgoub  Number of specimen. 2- Specimen container: Must be labeled with the correct information of the patient that match information on request form. 3- Specimen registration: after accepting specimen in reception then will give it lab number( code) Dr Eman mahgoub The usual numerical method of specimen identification is simply the year, expressed in two digits, with a sequential numbering system starting with one (1) and proceeding up to the final specimen of each year e.g. (215-24). In cases, multiple specimens from a single patient is received on the same day for analysis, give it a single laboratory number. Dr Eman mahgoub  Selection Second step after reception is selection.   Selection is choice of small part of large specimen for processing to be representative to the large one. Require macroscopic description of specimen As: Size, shape, color, weight, texture and cut surface appearance, for diagnostic information. Dr Eman mahgoub Dr Eman mahgoub  Selection room contain: Cutting board (non-absorbent) Forceps Scalped/ knifes / saw. Ink/ dye. Cassettes. Weighing scales. Pencil, ruler. Dr Eman mahgoub Dr Eman mahgoub Dr Eman mahgoub Dr Eman mahgoub Specimen dissection plans: Small samples: 1. Macroscopic description 2. Small biopsy rarely need dissection and can be processed as they present. 3. wrapped in paper in order to prevent the samples falling through the cassette perforations and being lost. 4. Eosin can be used as a marker for small samples in order to highlight them on the background of paper. 5. e.g. Endoscopic biopsy, Core biopsies, Skin biopsies. Dr Eman mahgoub Dr Eman mahgoub Intermediate and larger samples: 1. These must be described in terms of the width and depth. 2. Specimen lesion characteristics, e.g. nodule, ulcer or papule, color and the distance to the margins should be recorded. 3. Specimens managed in serial section, with Indian ink or another dye being applied to different surfaces in order to confirm the orientation or boundaries of the specimen. Dr Eman mahgoub Dr Eman mahgoub The several biopsies tank from intermediate and large sample are all labeled by the same lab number. Label either numerical using pencil or by bar code. Dr Eman mahgoub Dr Eman mahgoub Good luck Dr Eman mahgoub