Enzyme Purification and Assay Lecture Notes PDF
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Summary
These lecture notes describe enzyme purification and assay techniques. The document covers various topics, including kcat and SpC, continuous and discontinuous assays, and enzyme units of activity and specific activity. The notes also touch upon Michaelis-Menten Kinetics and the Law of Conservation of Mass in the context of enzyme reactions.
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Lecture #3: Enzyme Purification and Enzyme Assay 1. Review of Lecture #2 2. kcat and SpC 3. Continuous and discontinuous assays 4. Enzyme units of activity and specific activity The law of conservation of mass dictates that Michaelis-Menten Kinetics Review...
Lecture #3: Enzyme Purification and Enzyme Assay 1. Review of Lecture #2 2. kcat and SpC 3. Continuous and discontinuous assays 4. Enzyme units of activity and specific activity The law of conservation of mass dictates that Michaelis-Menten Kinetics Review [E]0 = [E] + [ES] (7) which can be readily rearranged to yield: Knowing the KM value permits adjustment of assay conditions, one can vary [S] according [E] to the needs of assay, can then measure [E]0 or [S] 0 = [E]0 − [ES] (8) If: + of (8) into (6) yields: Substitution [S]([E] k1[S]
