Bio 400 Lab Exam 1 Study Guide PDF

Bio 400 Lab Exam 1 Study Guide- You may use one 3x5inch index card with notes. It is worth 75 points. Lab Safety rules, proper disposal, etc. Microscope- parts, magnification, measuring (unit size will be given), function of parts, focusing, use of immersion oil Bacteria morphology: shape of Bacillu...

Bio 400 Lab Exam 1 Study Guide- You may use one 3x5inch index card with notes. It is worth 75 points. Lab Safety rules, proper disposal, etc. Microscope- parts, magnification, measuring (unit size will be given), function of parts, focusing, use of immersion oil Bacteria morphology: shape of Bacillus cereus, Staphylococcus aureus, Escherichia coli Common lab techniques: fixing bacteria to a slide, aseptic technique, streak plate method Gram stain- steps, chemicals used, results. How do the following organisms gram stain? Bacillus cereus, Staphylococcus aureus, Escherichia coli. What is it testing? Acid fast stain-steps, chemicals used, possible results, what does it test for. What bacteria stain positive? What is it testing? Endospore stain- steps, possible results, what does it test for. What bacteria stain positive? What is it testing? Coliform testing- presumed and definitive tests- which each tests for, why do definitive test after presumed test. Results for definitive tests. What do coliform bacteria look like on EMB media? What do results mean? What is it testing? Carbohydrate fermentation- phenol red tests (glucose, sucrose, lactose). Possible results- what do positive and negative results mean? What is it testing? Catalase test. Possible results- what do positive and negative results mean? What is it testing? Decarboxylase test. Possible results- what do positive and negative results mean? What is it testing? Starch hydrolysis test. Possible results- what do positive and negative results mean? What is it testing? Gelatin hydrolysis test. Possible results- what do positive and negative results mean? What is it testing? Citrate test. Possible results- what do positive and negative results mean? What is it testing? Spirit Blue test. Possible results- what do positive and negative results mean? What is it testing? SIM- test. What does it stand for, possible results- what do positive and negative results mean? What is it testing? MR-VP. Possible results- what do positive and negative results mean? What is it testing? Kirby-Bauer method. Know the names of all the enzymes that are produced by bacteria in tests and what they breakdown. Microscope Lab Part 1- Microscope- parts, magnification (if given magnification of objective and compound lens, be able to calculate total magnification), measuring (unit size will be given), function of parts, focusing, use of immersion oil Measurement- mm and cm Types of microscopes- dissecting, compound light microscope, electron microscope (SEM and TEM)- when would you use each one?