L2 Microtechniques PDF

Introduction DR. DALIA EITA  Methods of preparation of light microscopic sections  Principles of staining with Hematoxylin & Eosin  Types of stains  Types of microscopes 1. Demonstrate the methods of tissue fixation and procedures used for tissue embedding. 2. Demons...

Introduction DR. DALIA EITA  Methods of preparation of light microscopic sections  Principles of staining with Hematoxylin & Eosin  Types of stains  Types of microscopes 1. Demonstrate the methods of tissue fixation and procedures used for tissue embedding. 2. Demonstrate the common staining methods, their rationale, and the usefulness of cytochemical and histochemical techniques. 3. difference between light and electron microscopes Other Methods of staining PAS stains carbohydrates and gives: a. Black color b. Magenta color c. Red color d. Orange color e. Blue color  Microscopy Types of Microscopes Light microscope student Electron microscope microscope Magnification Magnification up to 40,100,400,1000 50.000 Resolution power: It is the capacity of the microscope to reveal the details of a specimen. The resolution power It is measured as the smallest of LM: about 0.2 um distance between two particles (200nm) that can be differentiated from The resolution power each other as two separate of EM: about 1 nm particles Magnification of light microscope = Magnification of objective lense × Magnification of ocular lens. https://www.youtube.com/watch?app=desktop&v=FnOvLEaC4gg Micro technique for Electron Microscopy b) Scanning Electron microscope Allows 3 dimensional visualization of the surface of a fixed specimen