Histology Practical 1 PDF
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Mansoura University
Dr. Shalaby
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Summary
This document is a practical guide to histology, covering techniques like microtechniques and microscopy. It explains how to study body tissues using light and electron microscopes, and details the preparation of tissue samples.
Full Transcript
Histology practical 1 Microtechniques & Microscopy Histology Microtechniques & Microscopy How To Study Body Tissues? ① Specimen. ② Microscopy: Light microscope. Electron microscope. 1...
Histology practical 1 Microtechniques & Microscopy Histology Microtechniques & Microscopy How To Study Body Tissues? ① Specimen. ② Microscopy: Light microscope. Electron microscope. 1 Light Microscope (LM) Steps of preparing specimen for LM examination ① Preparing a tissue section. ② Staining the section: a. Routine stain: Hematoxylin and eosin (H&E) Basophilic Basophilic Acidophilic Acidophilic Cytoplasm Nucleus Cytoplasm Nucleus b. Special stains. Histology 2 Electron Microscope (EM) Transmission Electron Microscope Scanning Electron Microscope (TEM) (SEM) For detection of intracellular details For giving 3 dimensional (3 d) image of the surface Electron dense granules Electron lucent granules Dark components in electron microscopic White components in electron microscopic images images أسود أبيض
