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# Chapter 7 Notes **Binary Fission:** One cell becomes two. * **Generation time (doubling time):** Time required for a complete fission cycle, from parent cell to two daughter cells. **Growth Curve:** A predictable pattern of bacterial population growth in a closed system. * **Lag phase:** A "flat...

# Chapter 7 Notes **Binary Fission:** One cell becomes two. * **Generation time (doubling time):** Time required for a complete fission cycle, from parent cell to two daughter cells. **Growth Curve:** A predictable pattern of bacterial population growth in a closed system. * **Lag phase:** A "flat" period of growth. Newly inoculated cells require a period of adjustment, enlargement, and synthesis. Cells are not yet multiplying at their maximum rate. * **Exponential growth (logarithmic or log) phase:** Growth curve increases geometrically. Growth will continue as long as cells have adequate nutrients and the environment is favorable. * **Stationary phase:** * **Death phase:** A clear nutrient solution becomes turbid, or cloudy, as microbes grow in it. **Measuring Bacterial Population:** * **Direct cell count:** Measured microscopically. * **Coulter counter:** Electronically scans a fluid as it passes through a tiny pipette. * **Flow cytometer:** Works similarly to a Coulter counter, but can measure cell size and differentiate between live and dead cells.