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Questions and Answers
What is the main process by which one cell becomes two?
What is the main process by which one cell becomes two?
Which phase of the growth curve indicates that newly inoculated cells are adjusting and not yet multiplying rapidly?
Which phase of the growth curve indicates that newly inoculated cells are adjusting and not yet multiplying rapidly?
During which phase of the growth curve does bacterial population increase geometrically?
During which phase of the growth curve does bacterial population increase geometrically?
What method can be used to electronically count cells passing through a pipette?
What method can be used to electronically count cells passing through a pipette?
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What happens during the Death phase of bacterial growth?
What happens during the Death phase of bacterial growth?
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Study Notes
Chapter 7 Notes
- Binary fission: one cell divides into two
- Generation time (doubling time): time for one cell to divide into two
- Generation: population increases by a factor of two
Growth Curve
- Predictable population growth pattern in a closed system
- Lag phase: initial "flat" period; newly inoculated cells adjust, enlarge, and synthesize
- Exponential (log) phase: rapid growth; cells multiply at maximum rate; growth increases geometrically
- Stationary phase: growth rate slows down, nutrients become limited; environment becomes unfavorable
- Death phase: cells die at a faster rate than new cells are produced; environmental conditions become increasingly unfavorable
Measuring Microbial Growth
- Direct cell count: microscopic count
- Coulter counter: electronically counts cells as they pass through a small aperture
- Flow cytometer: a device that measures cell size & differentiates between live and dead cells; similar to Coulter counter
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Description
Explore the key concepts of microbial growth as outlined in Chapter 7, including binary fission, growth phases, and methods for measuring microbial populations. Understand the dynamics of population changes in a closed system and the technology used for cell counting.
