Microbial Growth and Measurement Chapter 7
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Questions and Answers

What is the main process by which one cell becomes two?

  • Fragmentation
  • Binary Fission (correct)
  • Budding
  • Mitosis

Which phase of the growth curve indicates that newly inoculated cells are adjusting and not yet multiplying rapidly?

  • Stationary phase
  • Lag phase (correct)
  • Death phase
  • Exponential phase

During which phase of the growth curve does bacterial population increase geometrically?

  • Death phase
  • Exponential growth phase (correct)
  • Stationary phase
  • Lag phase

What method can be used to electronically count cells passing through a pipette?

<p>Flow cytometer (D)</p> Signup and view all the answers

What happens during the Death phase of bacterial growth?

<p>The nutrient solution becomes cloudy (A)</p> Signup and view all the answers

Flashcards

Binary Fission

A method of asexual reproduction where one cell divides into two identical daughter cells.

Generation Time

The time it takes for a bacterial cell to completely divide into two daughter cells.

Lag Phase

The initial phase in a bacterial growth curve where cells are adjusting to the new environment and not yet multiplying at a maximum rate.

Exponential Growth Phase

The phase where bacterial cells multiply at an accelerated rate, doubling in number with each generation.

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Direct Cell Count

A method of measuring bacterial population by directly counting cells under a microscope.

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Study Notes

Chapter 7 Notes

  • Binary fission: one cell divides into two
  • Generation time (doubling time): time for one cell to divide into two
  • Generation: population increases by a factor of two

Growth Curve

  • Predictable population growth pattern in a closed system
  • Lag phase: initial "flat" period; newly inoculated cells adjust, enlarge, and synthesize
  • Exponential (log) phase: rapid growth; cells multiply at maximum rate; growth increases geometrically
  • Stationary phase: growth rate slows down, nutrients become limited; environment becomes unfavorable
  • Death phase: cells die at a faster rate than new cells are produced; environmental conditions become increasingly unfavorable

Measuring Microbial Growth

  • Direct cell count: microscopic count
  • Coulter counter: electronically counts cells as they pass through a small aperture
  • Flow cytometer: a device that measures cell size & differentiates between live and dead cells; similar to Coulter counter

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Description

Explore the key concepts of microbial growth as outlined in Chapter 7, including binary fission, growth phases, and methods for measuring microbial populations. Understand the dynamics of population changes in a closed system and the technology used for cell counting.

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