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BIO 121 - Industrial Microbiology University of the Philippines Baguio | Basic Techniques in Isolating, Purifying, Screening, and Identifying Microorganisms Basic Isolation and Cultivation Techniques any natural or man-made Shotgun environment. - specific Objective where microorganisms with desired...

BIO 121 - Industrial Microbiology University of the Philippines Baguio | Basic Techniques in Isolating, Purifying, Screening, and Identifying Microorganisms Basic Isolation and Cultivation Techniques any natural or man-made Shotgun environment. - specific Objective where microorganisms with desired characteristics were present in natural microflora. Culture-Dependent Methods Spread-Plating Selective media and inhibitors For in situ isolation Not detrimental to heat sensitive microorganisms. Cons: formation of coalesced colonies. Pour-Plating No pre-drying of agar required. Small and subsurface colonies might be overlooked. Liquid Media Selective media, inhibitors, or enriched media. Used widely for cultivation. Types of Media Selective - allows the growth of desired microorganisms but inhibits the growth of some microorganisms. sites Differential - allows the desired group of microorganisms to be visually distinguished from other microorganisms. supplemented with nutritious Enriched materials that encourages the growth of desired group of microorganisms. - Other Methods Serial dilution on To reduce the number of microbial species for plating techniques. Decide the best concentration and solvent to use. Detrimental to rare species. dilution A.Y. 2023-2024 1st Semester Cell Spray liquid cell suspension in atomized with sterile air leading to the scattering of cells onto an agar plate. Centrifugation separation according to size and density, for concentration of larger cells. Filtration Separate particulate contents according to size and shape. Membrane filters O O Filters from intertwined organic filters Polycarbonate filter. Optical Tweezers Optical trapping A focused light beam attracts the medium towards the center of the beam. Isolate cells using micropipette. Micropipette For single cell isolation Avoids cell damage and clean isolation. Phototaxis For isolation of flagellates that exhibit phototaxis. Principle: a focused light source at one end of the apparatus will be used to attract a phototactic cell allowing isolation of cell/s. Flow cytometry To count and analyze optical properties of cells. Sorting of cells. Fluorescence- activated cell sorting (FACS): sorts cells based on specific light scattering and fluorescent charact of cells. Isolation and Cultivation Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) Principle: micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW