12 Questions
What is the principle behind Optical Tweezers?
A focused light beam attracts the medium towards the center of the beam.
How does Fluorescence-activated cell sorting (FACS) work?
It sorts cells based on specific light scattering and fluorescent characteristics of cells.
What is the principle behind the Soil Substrate Membrane System (SSMS) method?
Micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate.
How does Phototaxis aid in cell isolation?
A focused light source at one end of the apparatus is used to attract a phototactic cell, allowing isolation of cell/s.
What is the purpose of Flow Cytometry in cell analysis?
To count and analyze optical properties of cells.
How does a Micropipette aid in single cell isolation?
It avoids cell damage and allows for clean isolation of single cells.
What are the pros and cons of using Spread-Plating for isolating microorganisms?
Pros: Not detrimental to heat sensitive microorganisms. Cons: formation of coalesced colonies.
Why might small and subsurface colonies be overlooked when using Pour-Plating?
No pre-drying of agar is required, which may cause small and subsurface colonies to be missed.
What is the purpose of using Liquid Media for cultivating microorganisms?
Liquid Media is used widely for cultivation and can contain selective media, inhibitors, or enriched media.
Differentiate between Selective and Enriched types of Media in microbiology.
Selective media allows the growth of desired microorganisms but inhibits others, while enriched media encourages the growth of specific microorganisms.
What is the purpose of Serial Dilution in the context of isolating microorganisms?
Serial dilution is used to reduce the number of microbial species for plating techniques, although it can be detrimental to rare species.
Describe the process of Cell Spray in isolating microorganisms.
Cell Spray involves spraying liquid cell suspension in atomized form with sterile air onto an agar plate.
Test your knowledge on techniques such as filtration, membrane filters, optical tweezers, and micropipette for isolating particles and cells based on size, shape, and light manipulation. Explore principles behind phototaxis and the use of focused light beams for trapping particles.
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