Filtration and Optical Manipulation Techniques Quiz

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Questions and Answers

What is the principle behind Optical Tweezers?

A focused light beam attracts the medium towards the center of the beam.

How does Fluorescence-activated cell sorting (FACS) work?

It sorts cells based on specific light scattering and fluorescent characteristics of cells.

What is the principle behind the Soil Substrate Membrane System (SSMS) method?

Micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate.

How does Phototaxis aid in cell isolation?

A focused light source at one end of the apparatus is used to attract a phototactic cell, allowing isolation of cell/s. Signup and view all the answers

What is the purpose of Flow Cytometry in cell analysis?

To count and analyze optical properties of cells. Signup and view all the answers

How does a Micropipette aid in single cell isolation?

It avoids cell damage and allows for clean isolation of single cells. Signup and view all the answers

What are the pros and cons of using Spread-Plating for isolating microorganisms?

Pros: Not detrimental to heat sensitive microorganisms. Cons: formation of coalesced colonies. Signup and view all the answers

Why might small and subsurface colonies be overlooked when using Pour-Plating?

No pre-drying of agar is required, which may cause small and subsurface colonies to be missed. Signup and view all the answers

What is the purpose of using Liquid Media for cultivating microorganisms?

Liquid Media is used widely for cultivation and can contain selective media, inhibitors, or enriched media. Signup and view all the answers

Differentiate between Selective and Enriched types of Media in microbiology.

Selective media allows the growth of desired microorganisms but inhibits others, while enriched media encourages the growth of specific microorganisms. Signup and view all the answers

What is the purpose of Serial Dilution in the context of isolating microorganisms?

Serial dilution is used to reduce the number of microbial species for plating techniques, although it can be detrimental to rare species. Signup and view all the answers