Gluconeogenesis Hormonal Regulation PDF

Gluconeogenesis Hormonal Regulation Modulated by fructose-2,6-bisphosphate + regulator of glycolysis -regulator of gluconeogenesis Fructose-6-Phosphate + ATP ? Fructose 2,6-bisphosphate Catalysed by 6-phosphofructo-2-kinase 6-phosphofructo-2-kinase is a bifunctional enzyme meaning it formds and degr...

Gluconeogenesis Hormonal Regulation Modulated by fructose-2,6-bisphosphate + regulator of glycolysis -regulator of gluconeogenesis Fructose-6-Phosphate + ATP ? Fructose 2,6-bisphosphate Catalysed by 6-phosphofructo-2-kinase 6-phosphofructo-2-kinase is a bifunctional enzyme meaning it formds and degrades fructose-2,6-bisphosphate It has both Kinase domain (6-phosphofructo-2-kinase) and Phosphatases domain (Fructose2,6-bisphosphate) Glucagon Gluconeogenesis regulation Favoured by high ATP and low AMP- when energy is needed, glycolysis is activated when there is plently of energy, gluconeogenesis is activated to produce the glucose. CAMP Citrate inhibits phosphofructokinase which converts converts fructose 6-phosphate to fructose 1,6-bisphosphate but we want to go the other way round so it inhibits it. Protein Kinase A (PKA) Acetyl CoA stimulates pyruvate carboxylase which converts pyruvate into oxaloacetate. Phosporlyation of bifuntional enzyme Activates Fructose-2,6-bisphosphatase and Inhibits 6-Phosphofructo-2-kinase Therefore, activates gluconeogenesis and inhibits glycolysis