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# Candida **Description of the organism:** * **Brownish birdseed/Niger seed agar:** This is a type of growth medium used to culture the organism and is colored due to enzymes. * **Phenol Oxidase or Laccase:** These enzymes are involved in the observed color change in the agar * **Confirmatory test...

# Candida **Description of the organism:** * **Brownish birdseed/Niger seed agar:** This is a type of growth medium used to culture the organism and is colored due to enzymes. * **Phenol Oxidase or Laccase:** These enzymes are involved in the observed color change in the agar * **Confirmatory test:** Latex agglutination test is used to confim the presence of the organism * **Treatment:** Liposomal amphotericin B with flucytosine is a treatment option **Microscopic Observations:** * **Curdy white discharge/PAP smear:** This result is observed of the discharge. * **Sheesh kebab effect:** Cells are arranged in a striking appearance. * **Cells in a line**: This is a sign of pseudohyphae. * **Budding and pseudohyphae:** Observe budding and pseudohyphal forms of Candida cells. * **Germ Tube Test:** Candida grows on a variety of agars, especially cream/past/yeasty SDA * **Growth on Chrome Agar:** * **Chlamydospores Formation on Corn Meal Agar:** * **Germ Tube Test:** Keep the candida with a growth factor for observation. * **Braude Phenomenon:** This refers to a characteristic reaction or appearance observed in the study of Candida * **Mother cells and budding cells:** Display the structure and development of the organism **Table of Candida Species Characteristics:** | Species | Germ Tube Test | Chlamydospores | Growth above 45°C | Growth in Hypertonic Saline | |-----------------|-----------------|-----------------|----------------------|---------------------------| | *Candida albicans* | Positive | Positive | Negative | Negative | | *Candida dubliniensis* | Positive | Positive | Negative | Negative | **Treatment:** * **Oral thrush, vulvovaginal candidiasis:** Topical nystatin. * **Severe candidiasis:** Fluconazole/nystatin. * **Deep candidiasis:** LAMB. * **Azoles** **Additional Notes:** * Image shows different microscopic views (high magnification) of the organism * Photographs of the organism grown on various agar plates are included.. * The table clearly differentiates *Candida albicans* from *Candida dubliniensis* based on common laboratory tests