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This document contains notes on biological topics, including experimental design, classification of organisms, chemistry of life, and macromolecules. The content appears to be study material, potentially for a secondary school level biology class or a similar preparation. The document likely comes from a textbook, work book or educational resource rather than a formal past paper.

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‭1. Experimental Design and Bias‬ ‭When a properly controlled experiment lacks bias:‬ ‭‬ C ‭ ontrol group‬‭: Comparison with experimental groups.‬ ‭‬ ‭Random sampling‬‭: Ensures subjects are selected without‬‭bias.‬ ‭‬ ‭Blinded studies‬‭: Prevents influence from researchers‬‭or pa...

‭1. Experimental Design and Bias‬ ‭When a properly controlled experiment lacks bias:‬ ‭‬ C ‭ ontrol group‬‭: Comparison with experimental groups.‬ ‭‬ ‭Random sampling‬‭: Ensures subjects are selected without‬‭bias.‬ ‭‬ ‭Blinded studies‬‭: Prevents influence from researchers‬‭or participants.‬ ‭Types of Systematic Bias:‬ ‭‬ ‭Measurement bias‬‭: Inaccurate tools or methods.‬ ‭○‬ ‭Avoidance‬‭: Calibrate instruments, and standardize‬‭measurements.‬ ‭‬ ‭Selection bias‬‭: Non-random selection of subjects.‬ ‭○‬ ‭Avoidance‬‭: Random sampling, stratified sampling.‬ ‭‬ ‭Confirmation bias‬‭: Researchers favor data that supports‬‭their hypothesis.‬ ‭○‬ ‭Avoidance‬‭: Peer review, open data sharing, objective‬‭analysis.‬ ‭Importance of Peer Review:‬ ‭‬ E ‭ nsures validity, reliability, and credibility of research.‬ ‭‬ ‭Helps to identify errors, omissions, or biases in the study.‬ ‭2. Classification of Organisms‬ ‭Taxonomic Ranks (in order of specificity):‬ ‭1.‬ D ‭ omain‬‭→ 2.‬‭Kingdom‬‭→ 3.‬‭Phylum‬‭→ 4.‬‭Class‬‭→ 5.‬‭Order‬‭→ 6.‬‭Family‬‭→ 7.‬‭Genus‬ ‭→ 8.‬‭Species‬ ‭3 Domains and Characteristics:‬ ‭‬ A ‭ rchaea‬‭: Single-celled, no nucleus, extreme environments.‬ ‭‬ ‭Bacteria‬‭: Single-celled, no nucleus, common bacteria.‬ ‭‬ ‭Eukarya‬‭: Cells with a nucleus, including plants, animals,‬‭fungi, and protists.‬ ‭6 Kingdoms and Characteristics:‬ ‭.‬ A 1 ‭ rchaebacteria‬‭: Prokaryotic, extremophiles.‬ ‭2.‬ ‭Eubacteria‬‭: Prokaryotic, common bacteria.‬ ‭3.‬ ‭Protista‬‭: Single-celled, eukaryotic, diverse.‬ ‭.‬ F 4 ‭ ungi‬‭: Multicellular, eukaryotic, absorb nutrients.‬ ‭5.‬ ‭Plantae‬‭: Multicellular, eukaryotic, photosynthetic.‬ ‭6.‬ ‭Animalia‬:‭ Multicellular, eukaryotic, heterotrophic.‬ ‭Classifying Using a Dichotomous Key:‬ ‭‬ ‭Use a series of yes/no questions to identify organisms.‬ ‭3. Chemistry of Life‬ ‭Properties of Water:‬ ‭‬ ‭Polar, high specific heat, cohesive, adhesive, solvent for many substances.‬ ‭Parts of an Atom:‬ ‭‬ P ‭ rotons‬‭(positive),‬‭Neutrons‬‭(neutral),‬‭Electrons‬‭(negative).‬ ‭‬ ‭Atoms are the smallest units of elements, compounds are made of atoms, and mixtures‬ ‭consist of two or more substances combined physically.‬ ‭Types of Bonds:‬ ‭ ‬ ‭Covalent Bonds‬‭: Sharing of electrons.‬ ‭‬ ‭Ionic Bonds‬‭: Transfer of electrons.‬ ‭Functional Groups in Molecules:‬ ‭‬ ‭Hydroxyl (-OH), Carboxyl (-COOH), Amino (-NH2), Phosphate (-PO4), etc.‬ ‭4. Macromolecules‬ ‭Functions of Macromolecules:‬ ‭‬ ‭ arbohydrates‬‭: Energy storage, structure (e.g., starch,‬‭cellulose).‬ C ‭‬ ‭Lipids‬‭: Long-term energy storage, cell membranes,‬‭signaling (e.g., fats, phospholipids).‬ ‭‬ ‭Proteins‬‭: Enzymes, structure, transport, signaling‬‭(e.g., enzymes, antibodies).‬ ‭‬ ‭Nucleic Acids‬‭: Genetic information (e.g., DNA, RNA).‬ ‭Types of Macromolecules:‬ ‭‬ ‭ onosaccharides‬‭: Simple sugars (e.g., glucose).‬ M ‭‬ ‭Disaccharides‬‭: Two sugar units (e.g., sucrose).‬ ‭‬ ‭Polysaccharides‬‭: Multiple sugar units (e.g., starch).‬ ‭‬ ‭Triglycerides‬‭: Fats, energy storage.‬ ‭‬ ‭Fatty Acids‬‭: Building blocks of lipids.‬ ‭‬ ‭Phospholipids‬‭: Cell membrane structure.‬ ‭‬ ‭Peptides/Polypeptides‬‭: Short/long chains of amino‬‭acids.‬ ‭‬ ‭DNA/RNA‬‭: Genetic material, involved in protein synthesis.‬ ‭Dehydration Synthesis & Hydrolysis:‬ ‭‬ D ‭ ehydration‬‭: Formation of a bond by removing water‬‭(e.g., monosaccharide →‬ ‭disaccharide).‬ ‭‬ ‭Hydrolysis‬‭: Breaking of a bond by adding water (e.g.,‬‭disaccharide →‬ ‭monosaccharides).‬ ‭5. Enzyme Activity‬ ‭Enzyme Impact:‬ ‭‬ ‭Lowers activation energy for chemical reactions.‬ ‭Types of Reactions:‬ ‭‬ A ‭ nabolic‬‭: Build larger molecules (requires energy).‬ ‭‬ ‭Catabolic‬‭: Break down molecules (releases energy).‬ ‭Factors Affecting Enzyme Activity:‬ ‭‬ T ‭ emperature‬‭,‬‭pH‬‭,‬‭Substrate concentration‬‭,‬‭Enzyme concentration‬‭.‬ ‭‬ ‭Competitive Inhibition‬‭: Inhibitor competes with substrate‬‭for the active site.‬ ‭6. Digestive System‬ ‭Digestive Organs & Their Roles:‬ ‭‬ M ‭ outh‬‭: Mechanical digestion (chewing), chemical digestion‬‭(saliva).‬ ‭‬ ‭Stomach‬‭: Protein digestion (pepsin), acidic environment.‬ ‭‬ ‭Small Intestine‬‭: Nutrient absorption (villi).‬ ‭‬ ‭ arge Intestine‬‭: Water absorption, formation of feces.‬ L ‭‬ ‭Pancreas‬‭: Secretes digestive enzymes.‬ ‭‬ ‭Liver‬‭: Produces bile.‬ ‭‬ ‭Gallbladder‬‭: Stores bile.‬ ‭‬ ‭Rectum/Anus‬‭: Expulsion of waste.‬ ‭Enzymes Involved in Digestion:‬ ‭‬ ‭ mylase‬‭: Breaks down starch (mouth, pancreas).‬ A ‭‬ ‭Pepsin‬‭: Breaks down proteins (stomach).‬ ‭‬ ‭Lipase‬‭: Breaks down lipids (pancreas).‬ ‭‬ ‭Protease‬‭: Breaks down proteins (stomach, pancreas).‬ ‭‬ ‭Lactase‬‭: Breaks down lactose (small intestine).‬ ‭7. Photosynthesis & Cellular Respiration‬ ‭Photosynthesis:‬ ‭‬ E ‭ quation‬‭: 6CO2 + 6H2O → C6H12O6 + 6O2‬ ‭‬ ‭Light-dependent reactions‬‭: Occur in thylakoid membranes, and produce ATP and‬ ‭NADPH.‬ ‭‬ ‭Light-independent reactions (Calvin Cycle)‬‭: Occur in the stroma, use ATP and‬ ‭NADPH to synthesize glucose.‬ ‭Cellular Respiration:‬ ‭‬ E ‭ quation‬‭: C6H12O6 + 6O2 → 6CO2 + 6H2O + ATP‬ ‭‬ ‭Aerobic‬‭: Requires oxygen, and produces more ATP (includes Glycolysis, Krebs Cycle,‬ ‭ETC).‬ ‭‬ ‭Anaerobic‬‭: No oxygen, less ATP (Fermentation).‬ ‭8. Biogeochemical Cycles‬ ‭Carbon Cycle:‬ ‭‬ P ‭ lants and animals need carbon for energy and growth.‬ ‭‬ ‭Carbon enters the atmosphere through respiration, combustion, and decay.‬ ‭‬ ‭Carbon is removed through photosynthesis.‬ ‭Nitrogen Cycle:‬ ‭‬ N ‭ itrogen-fixing bacteria convert atmospheric nitrogen into forms plants can use‬ ‭(nitrates).‬ ‭‬ ‭Denitrifying bacteria convert nitrates back to nitrogen gas.‬ ‭Water Cycle:‬ ‭‬ ‭Water is moved through evaporation, transpiration, condensation, and percolation.‬ ‭Phosphorus Cycle:‬ ‭‬ P ‭ hosphorus is found in rocks, soil, and water. Plants absorb phosphate, and it returns to‬ ‭the environment through decay and waste.‬ ‭9. Root Words‬ ‭Definitions:‬ ‭‬ ‭ acro-‬‭: Large‬ M ‭‬ ‭Mono-‬‭: One‬ ‭‬ ‭Di-‬‭: Two‬ ‭‬ ‭Poly-‬‭: Many‬ ‭‬ ‭Sacchar-‬‭: Sugar‬ ‭‬ ‭-mer‬‭: Part‬ ‭‬ ‭Hydr(o)-‬‭: Water‬ ‭‬ ‭-lys-‬‭: Break‬ ‭‬ ‭Glyc-‬‭: Sweet‬ ‭‬ ‭-ose‬‭: Sugar‬ ‭‬ ‭-ic‬‭: About‬ ‭‬ ‭Syn-‬‭: Together‬ ‭‬ ‭Oxy-‬‭: Oxygen‬ ‭‬ ‭-phobic‬‭: Fear of‬ ‭‬ ‭-Philip‬‭: Love of‬ ‭‬ ‭Hem-‬‭: Blood‬

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