EXPERIMENT 1 PDF
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Uploaded by ZippyCharoite
Iqra University
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Summary
This document describes a chemistry experiment to identify reducing sugars using the Benedict's test. The experiment involves taking a test solution, adding Benedict's reagent, and heating it in a water bath. The presence of reducing sugars is confirmed by the formation of a brick-red colored precipitate.
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EXPERIMENT#1 OBJECT: To perform Benedict test for identification of carbohydrates REQUIREMENTS: Test tubes, dropper, test tube holder, Beaker, sample of carbohydrate, Benedict reagent, water bath PRINCIPLE Benedict reagent/solution is a complex mixture of sodium citrate, sodium carbonate, and th...
EXPERIMENT#1 OBJECT: To perform Benedict test for identification of carbohydrates REQUIREMENTS: Test tubes, dropper, test tube holder, Beaker, sample of carbohydrate, Benedict reagent, water bath PRINCIPLE Benedict reagent/solution is a complex mixture of sodium citrate, sodium carbonate, and the of copper sulfate pentahydrate (CuSO4.5H2O) in distilled water. When a reducing sugar is subjected to heat in the presence of an alkali, it gets converted into an enediol (which is a relatively powerful reducing agent). Therefore, when reducing sugars are present in the sample, the cupric ions (Cu2+) in Benedict’s reagent are reduced to cuprous ions (Cu+). These cuprous ions form copper(I) oxide with the reaction mixture and precipitate out as a brick-red coloured complex. PROCEDURE: Take 1ml of the test solution in a washed and clean test tube Add 2 ml of Benedict’s reagent and mix well Heat on hot/boiling water bath for approximately 3 minutes or until the visible color change occur. OBSERVATIONS Formation of orange/red colored precipitate. RESULT The development of a brick-red colored precipitate of cuprous oxide confirms the presence of reducing sugars in the given sample.
