Glucose Benedict's Test PDF
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Summary
This document describes the Benedict's test for glucose and defines monomers and polymers. It includes detailed instructions for the test procedure, and discusses biological molecules in a science course context.
Full Transcript
Use your knowledge and the image below to describe what a ‘monomer’ and a ‘polymer’ might be. Mono means one. Monobrow and Monocle are uses of the prefix “mono” Poly means many Polygons are shapes with more than one side Single simple units Chain of mo...
Use your knowledge and the image below to describe what a ‘monomer’ and a ‘polymer’ might be. Mono means one. Monobrow and Monocle are uses of the prefix “mono” Poly means many Polygons are shapes with more than one side Single simple units Chain of monomers bonded together Lesson – Biological Molecules - Glucose Lesson outcomes: Bronze – State the meaning of monomer and polymer. Silver – Describe the test for glucose. Gold – Complete the Benedict’s test. Elements: Carbohydrate structure Carbon Hydrogen Oxygen Structure: Carbohydrates are long chains (polymers) of small sugar molecules (monomers) Carbohydrate (e.g. starch and glycogen) = polysaccharide (polymer) Simple sugar (e.g. glucose) = mono saccharide (monomer) one sugar molecule (monomer) carbohydrate molecule (polymer) Benedict's test Benedict’s test is used to test for simple sugars, for example glucose. YOU HAVE TO LEARN THE METHOD! 1. Prepare a sample of the food. 2. Put 5 ml of the food sample into a test tube. 3. Add 5 ml of Benedict’s solution, using a syringe, and mix. 4. Place the test tube in a water bath at 80oC for 10 mins. 5. Record the colour after 10 mins. Negative result (no glucose) – blue Positive results: Low concentration of glucose – green/yellow Medium concentration of glucose – orange High concentration of glucose – brick red Video to help https://www.youtube.com/watch?v=13H1urX3gxI Complete the Benedict’s test for Glucose. You must wear goggles at all times! 1. Prepare a sample of the food. 2. Put 5 ml of the food sample into a test tube. 3. Add 5 ml of Benedict’s solution, using a syringe, and mix. 4. Place the test tube in a water bath at 80 oC for 10 mins. 5. Record the colour after 10 mins. And finally…. How to answer exam questions. If the question says “Design”, ”Plan” or “Include experimental details” C – Change (independent variable) O – organisms – not relevant for today. R – Repeat M – Measure (dependent variable) S – Same (control variable).