Electrophoresis.pptx

Electrophoresis  Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge. An electric current is used to move the molecules through a gel or other matrix  Electrophoresis involves running a current through a gel...

Electrophoresis  Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge. An electric current is used to move the molecules through a gel or other matrix  Electrophoresis involves running a current through a gel containing the molecules of interest. Based on their size and charge, the molecules will travel through the gel in different directions or at different speeds, allowing them to be separated from one another.  Electrophoresis is a technique used to separate molecules in a gel or fluid using an electric field. The rate and direction of particle movement in the electric field depends on the molecule's size and electric charge. Usually electrophoresis is used to separate macromolecules, such as DNA, RNA  There are several basic steps to performing gel electrophoresis that will be described below; 1) Pouring the gel, 2) Preparing your samples, 3) Loading the gel, 4) Running the gel (exposing it to an electric field) and 5) Staining the gel  At one end, the gel has pocket-like indentations called wells, which are where the DNA samples  Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest  The different fractions of the serum proteins separate usually into 5 bands, as – the albumin, α1, α2, β, and the γ globulin fractions. In the interpretation of SPEP, more attention is given to the gamma region, which is mainly composed of Immunoglobulin