BIOTECHNOLOGY (I & II) PDF

TG: @Chalnaayaaar Biotechnology : (I & II) Exercise - I PRINCIPLES AND PROCESSES 10. Which vector is commonly used in the 1. Agrobacterium tumefaciens used in Genetic transfer of gene in a crop plant ? engineering for :-...

TG: @Chalnaayaaar Biotechnology : (I & II) Exercise - I PRINCIPLES AND PROCESSES 10. Which vector is commonly used in the 1. Agrobacterium tumefaciens used in Genetic transfer of gene in a crop plant ? engineering for :- (1) Plasmids of B. Subtilis (1) DNA-mapping (2) Bacteriophages (2) DNA-modification (3) Ti-plasmids of Agrobacterium (3) Gene transfer (4) E. coli Phages (4) DNA finger printing 11. The tumour indusing capacity of 2. Who isolated the first restriction Agrobacterium tumaefaciens is located in endonucleases? extrachromosomal plasmid and called:- (1) Temin & Baltimore (2) Sanger (1) Ti – plasmid (3) Smith (4) Paul berg (2) Ri - plamid 3. Genetic engineering is :- (3) Lambda phage (1) Study of extra nuclear gene (4) Plasmid PBR 322 (2) Manipulation of genotype by artificial 12. Genetic engineering aims at :- method (1) Destroying wild gene (3) Manipulation of RNA (2) Preserving defective gene (4) Manipulation of enzymes (3) Curing human disease by introducing new 4. Polymerase chain reaction technology (PCR- gene technique) is used for :- (4) All the above (1) DNA identification (2) DNA repair 13. A piece of nucleic acid using to find out a gene, (3) DNA amplification (4) Cleave DNA 5. Which structure involved in genetic by forming hybrid with it, is called as:- engineering? (1) c – DNA (1) Plastid (2) Plasmid (2) DNA probe (3) Codon (4) None (3) Sticky end 6. Which of the following is the example of (4) Blunt end chemical scissors? 14. Taq - polymerase which is used for (1) Eco - RI (2) Hind - III amplification of DNA related with: - (3) Bam - I (4) All the above (1) Hybridoma technique 7. Restriction endonucleases are used in genetic (2) PCR-technique engineering because: - (3) Gene cloning (1) They can degrade harmful proteins (4) r-DNA technology (2) They can join DNA fragments 15. What is true for plasmid? (3) They can cut DNA at variable site (1) Plasmids are widely used in gene transfer (2) These are found in virus (4) They can cut DNA at specific base (3) Plasmid contains gene for vital activities sequences (4) These are main part of chromosome 8. When the genotype of an organism is 16. Which of the following cuts the DNA from improved by the addition of foreign gene, the specific places? process is called :- (1) Restriction endonuclease (1) Tissue culture (2) Genetic diversity (2) Ligase (3) Genetic engineering (4) Plastic surgery (3) Exonuclease 9. A genetically manipulated organism (4) Alkaline phosphate containing in its genome one or more 17. Manipulation of DNA in genetic engineering inserted gene of another species is called: - became possible due to the discovery of: - (1) Transposon (1) Restriction endonuclease (2) Gene expression (2) DNA ligase (3) Transgenic organism (3) Transcriptase (4) Retroposons (4) Primase TG: @Chalnaayaaar  Digital www.allendigital.in [ 55 ]  TG: @Chalnaayaaar NEET : Biology 18. Which one of the following has found 26. Restriction enzymes are :- extensive use in genetic engineering work in (1) Not always required in genetic plants? engineering (1) Bacillus coagulens (2) Essential tool in genetic engineering (2) Agrobacterium tumefaciens. (3) Nucleases that cleave DNA at specific sites (3) Clostridium septicum (4) (2) and (3) both (4) Xanthomonas citri 27. Electroporation procedure involves :- 19. Restriction endonucleases: - (1) Fast passage of food through sieve pores (1) Are enzymes which cleave DNA at specific sites in phloem elements with the help of (2) Make DNA complementary to an existing electric stimulation. DNA or RNA (2) Opening of stomatal pores during night by (3) Cut or join DNA fragments artificial light (4) Are required in vectorless direct gene (3) Making transient pores in the cell transfer. membrane to introduce gene constructs 20. Restriction endonucleases :- (4) Purification of saline water with the help (1) Are synthesized by bacteria as part of of a membrane system. their defense mechanism 28. Which of the following restriction (2) Are present in mammalian cells for endonuclease enzyme produce blunt end in degradation of DNA when the cell dies DNA :- (3) Are used in genetic engineering for  ligating two DNA molecules (1) Bam HI G G A T C C (4) Are used for invitro DNA synthesis CC TAGG 21. The Ti plasmid, is often used for making  transgenic plants. This plasmid is found in :-  (1) Yeast (2) Azotobacter (2) Eco RI G A A T T C (3) Rhizobium (4) Agrobacterium C TTAAG 22. Which of the following is the example of direct gene transfer?  (1) Microinjection  (3) Hae III G G C C (2) Electroporation CCGG (3) Particle gun  (4) All the above (4) All the above 23.. How many copies of DNA sample are 29. A bacterium modifies its DNA by adding produced in PCR technique after 6–cycle? methyl groups to the DNA, It does so to :- (1) 4 (2) 32 (3) 64 (4) 16 (1) Clone its DNA 24. Thermal cycle takes place in which technique (2) Be able to transcribe many genes (1) Gel electrophoresis simultaneously (2) PCR-technique (3) Turn its gene on (3) Centrifugation (4) Protect its DNA from its own restriction (4) Southern blotting enzyme 25. BAC and YAC are :- 30. The restriction enzyme Eco RI has the (1) Natural DNA obtained from bacteria and property of:- yeast (1) endonuclease activity (2) Useful vectors for eukaryotic gene (2) exonuclease activity transfer (3) ligation activity (3) Artificial DNA obtained from bacteria and (4) correcting the topology of replicating yeast (4) (2) & (3) both DNA TG: @Chalnaayaaar [ 56 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 31. DNA ligase is an enzyme that catalyses the:- 39. PCR proceeds in three distinct steps governed (1) splitting of DNA threads into small bits by temperature they are in order of:- (2) joining of the fragments of DNA (1) Denaturation, Annealing, Synthesis (3) denaturation of DNA (2) Synthesis, Annealing, Denaturation (4) synthesis of DNA 32. Agrobacterium tumefaciens contains a (3) Annealing, Synthesis, Denaturation large plasmid, which induces tumour in the (4) Denaturation, Synthesis, Annealing plants it is termed as :- 40. What is the source of the Ti (Tumor inducing) (1) Ti plasmid plasmid which is modified and used as a (2) Ri plasmid (3) Recombinant plasmid cloning vector to deliver the desirable genes (4) Shine Delgrano sequence into plant cells ? 33. More advancement in genetic engineering is (1) Agrobacterium tumifaciens due to :- (2) Thermophilus aquaticus (1) Restriction endonuclease (3) Pyrococcus furiosus (2) Reverse transcriptase (4) Aedes aegypti (3) Protease (4) Zymase 41. The thermostable enzymes, 'Taq' isolated 34. Which of the follwing is used as a best genetic from thermophilic bacteria is :- vector in plants? (1) RNA polymerases (1) Bacillus thuriengenesis (2) DNA polymerases (2) Agrobacterium tumifaciens (3) Restriction endonucleases (3) Pseudomonas putida (4) DNA ligases (4) All of these 35. Which of the following enzyme is used to join 42. The term "molecular scissors" generally DNA fragments? refers to:- (1) Terminase (1) DNA polymerases (2) Endonuclease (2) RNA polymerases (3) Ligase (3) Restriction endonucleases (4) DNA polymerase (4) DNA ligases 36. A kind of Biotechnology involving manipulation of DNA is :- 43. In the PCR technology the DNA segment is (1) DNA replication replicated over a billion times. This repeated (2) Genetic engineering replications catalyzed by the enzyme :- (3) Denaturation (1) RNA polymerase (4) Renaturation (2) Taq polymerase 37. What is true of plasmid ? (3) DNA dependent RNA polymerase (1) Found in viruses (4) Primase (2) Contains genes for vital activities (3) Part of nuclear chromosome 44. The restriction enzyme(s) used in recombinant (4) Widely used in gene transfer DNA technology making staggered cuts in 38. A suitable vector for gene cloning in higher DNA leaving sticky ends is/are :- organism is :- (1) EcoR I (1) Baculovirus (2) Hind III (2) Retrovirus (3) BamH I (3) Salmonella typhimurium (4) Neurospora crassa (4) All of the above TG: @Chalnaayaaar  Digital www.allendigital.in [ 57 ]  TG: @Chalnaayaaar NEET : Biology 45. Restriction enzyme Eco RI cuts the DNA 52. An antibiotic resistance gene of plasmid between bases G and A only when the vector which get inactivated due to insertion of alien DNA, helps in the selection of:- sequence in DNA is:- (1) Transformants (1) GATATC (2) GAATTC (2) Recombinants (3) GATTCC (4) GAACTT (3) Non-Transformants 46. 'Transgenic' plants are produced by :- (4) (2) & (3) both (1) Inducing gene mutation 53. In which type of bioreactor air bubbles (2) Arresting spindle fibre formation dramatically increases the oxygen transfer area? (3) Deleting sex chromosomes (1) Simple stirred tank bioreactor (4) Introducing foreign genes (2) Sparged stirred tank bioreactor 47. For a DNA to function as a cloning vector the (3) Both (1) & (2) most essential requirement is :- (4) None of these (1) multiple restriction sites 54. Genetic modification (GM) has been used to:- (2) several selectable markers (1) Create tailor made plants (3) circular nature (2) Supply alternative resources to industries (4) 'ori' sequence (3) Enhanced nutritional value of food. 48. According to EFB, "The integration of natural (4) All of the above science and organisms, cells, parts thereof 55. The choice of Bt-gene for experiment depends and molecular analogues for products and upon:- services," is known as:- (1) The host plant/crop (1) Biochemistry (2) Targeted pest/insect (2) Bioinformatics (3) Bacillus strain (3) Biotechnology (4) 1 & 2 both (4) Biology 56. In nematode resistance by RNA interference, 49. The stickiness of DNA ends facilitates the some specific genes were introduced which form dsRNA. These were introduced in:- action of which enzyme :- (1) Nematode (2) Host plant (1) DNA polymerase (3) Agrobacterium (4) All of these (2) DNA Ligase 57. Select the incorrect match:- (3) Restriction endonuclease (1) Transgenic mice - Polio vaccine (4) Alkaline phosphatase (2) Rosie cow -  lactalbumin gene 50. Which technique is used to check the (3) ssDNA/RNA probe - Gene therapy progression of restriction enzyme digestion? (4) PCR - Molecular diagnosis (1) PCR 58. In EcoR I, R is stand for :- (2) Gel electrophoresis (1) Strain (2) Species (3) Southern Blotting (3) Genus (4) Order (4) Staining 59. Restriction endonucleases are used in genetic 51. In gel electrophoresis, at which end of the gel the sample is loaded? engineering to form:- (1) In the wells (1) Recombinant molecule of protein (2) Towards positive electrode (2) Recombinant molecule of DNA (3) Towards negative electrode (3) Recombinant molecule of protein & DNA (4) (1) & (3) both (4) Recombinant cell TG: @Chalnaayaaar [ 58 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 60. Which instrument is used for the separation 68. Which of the following enzyme is known as of DNA fragments? molecular scissors? (1) PCR (1) Ligase (2) DNA polymerase (2) Gel electrophoresis (3) Restriction enzyme (4) Helicase (3) Bioreactor 69. Which of the following is not required in PCR? (4) Restriction endonuclease (1) DNA primer (2) DNA template 61. Which of following feature is not necessary (3) RNA primer (4) Taq polymerase for cloning vector? 70. The substrate for restriction enzyme is :- (1) Origin of replication (1) Single stranded RNA (2) High copy number (2) Proteins (3) Selectable marker (3) Double stranded DNA (4) Cloning sites (4) Single stranded DNA 62. Which of the following is not true for cloning 71. In recombinant DNA technology, the term vector? vectors refers to :- (1) more than two origin site of replication (1) the enzyme that cuts DNA into restriction (2) vector should have selectable marker gene fragments (3) single recognition site for the commonly (2) the sticky end of a DNA fragment used restriction enzyme (3) a plasmid used to transfer DNA into a (4) pBR-322 have tetracycline resistance gene living cell 63. Transformation is a procedure through (4) a DNA probe used to identify a particular which:- gene (1) A piece of DNA is introduced in a host 72. pBR322 which is frequently used as a vector bacterium for cloning gene is:– (2) A piece of DNA is introduced in a vector (1) an original bacterial plasmid (3) A piece of DNA is introduced from protein (2) a modified bacterial plasmid (4) All of these (3) a viral genome 64. Second letter of the name of restriction (4) a transposon endonuclease came from the :- APPLICATION OF BIOTECHNOLOGY (1) Genus of organism 73. Genetically engineered bacteria have been (2) Species of organism used in commercial production of :- (3) Family of organism (1) Thyroxin (4) Class of organism (2) Testosterone 65. To isolate DNA from fungi we have to break (3) Human insulin the wall. This is done by:- (4) Melatonium 74. Important objective of biotechnology in (1) Lysozyme (2) Cellulose agriculture section is :- (3) Invertase (4) Chitinase (1) To produce pest resistant varieties of 66. Which of the following enzyme will get plants inactivated in insertional inactivation? (2) To increase the nitrogen contant (1) Transacetylase (2) Permease (3) To decrease the seed number (4) To increase the plant weight (3) -galactosidase (4) Taq-polymerase 75. The name of drug used in cancer treatment 67. In presence of chromogenic substrate produced by biotechnology is:- recombinant bacteria gives:- (1) Red coloured colonies (1) Interferon (2) Colourless colonies (2) [HGH] Human growth hormone (3) Blue colonies (3) TSH (4) Green colonies (4) Insulin TG: @Chalnaayaaar  Digital www.allendigital.in [ 59 ]  TG: @Chalnaayaaar NEET : Biology 76. The prerequisites for biotechnological 85. A giant rat is formed in the laboratory, what production of antibiotics is :- is the reason :- (1) To search an antibiotic producing (1) Gene mutation (2) Gene synthesis microorganism (3) Gene manipulation (4) Gene replication (2) To isolate the antibiotic gene 86. Cultivation of Bt cotton has been much in the (3) To join antibiotic gene with E.coli plasmid news. The prefix ‘‘Bt’’ means :- (4) All of the above (1) ‘‘Barium – treated’’ cotton seeds. 77. Modern biotechnology consist :- (2) ‘‘Bigger thread’’ variety of cotton with (1) Genetic engineering (2) tissue culture batter tensile strength. (3) Microbiology (4) All the above (3) Produced by ‘‘biotechnology’’ using 78. First artificially synthesized hormone is :- restriction enzymes and ligases. (4) Carrying an endotoxin gene from Bacillus (1) Secretin (2) Insulin thuringiensis. (3) Glucagon (4) Renin 87. cry-gene which synthesize crystal protein 79. The protein products of the following Bt toxin isolated from :- genes cryIAc and cryIIAb are responsible for (1) Bacillus thuriengensis controlling:- (2) Rhizobium (1) Bollworm (2) Roundworm (3) Bacillus polymyxa (3) Moth (4) Fruit fly (4) Clostridium 80. A transgenic rice (Golden rice) has been 88. Which of the following combination of risk developed for increased content of :- are associated with genetically modified food? (1) Vitamin A (2) Vitamin B1 (1) Toxicity (3) Vitamin C (4) Vitamin D (2) Allergic reaction 81. During the processing of the prohormone (3) Antibiotic resistance in microorganism "proinsulin" into the mature "insulin" :- present in alimentary canal (1) C-peptide is added to proinsulin (4) All the above (2) C-peptide is removed from proinsulin 89. Gene therapy first used in the treatment of:- (3) B-peptide is added to proinsulin (1) Albinism (2) Haemophilia (4) B-peptide is removed from proinsulin (3) SCID (4) LIQID 82. A genetically engineered bacteria used for 90. Bt–cotton is resistant for :- clearing oil spills is :- (1) Round–worm (2) Fluke–worm (1) Escherischia coli (3) Boll–worm (4) Pin–worm (2) Bacillus subtilis 91. Genetically engineered human insulin is (3) Agrobacterium tumifaciens called :- (4) Pseudomonas putida (1) Humulin (2) Haematin 83. First transgenic plant :- (3) Hybridoma (4) Hybrid (1) Potato (2) Tomato 92. The C-peptide is :- (3) Tobacco (4) Maize (1) not present in proinsulin 84. E. coli are used in production of :- (2) present in mature insulin (1) Rifampicin (2) LH (3) removed during maturation of insulin (3) Ecdyson (4) Interferon (4) also present in artificial insulin TG: @Chalnaayaaar [ 60 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 93. GEAC makes decisions regarding :- 95. The use of bio-resources by multinational (1) the validity of GM research companies & other organisations without (2) the safety of introducing GM organisms proper authorisation from the countries & for public services people concerned, is known as :– (3) the validity of biopatents (1) Biopatent (2) Biopiracy (4) more than one options are correct (3) Biowar (4) Biodiversity 94. The Indian parliament has recently cleared 96. Which of the following peptide chain in not which amendment of the Indian patents bill:- present in mature insulin? (1) 1st (2) 2nd (1) A-peptide (2) B-peptide (3) 3rd (4) 4th (3) C-peptide (4) A & B peptide EXERCISE-I (Conceptual Questions) ANSWER KEY Question 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Answer 3 3 2 3 2 4 4 3 3 3 1 3 2 2 1 Question 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Answer 1 1 2 1 1 4 4 3 2 4 4 3 3 4 1 Question 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 Answer 2 1 1 2 3 2 4 2 1 1 2 3 2 4 2 Question 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Answer 4 4 3 2 2 4 2 2 4 4 2 3 1 2 2 Question 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 Answer 2 1 1 2 4 3 2 3 3 3 3 2 3 1 1 Question 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 Answer 4 4 2 1 1 2 4 3 4 3 4 1 4 3 3 Question 91 92 93 94 95 96 Answer 1 3 4 2 2 3 TG: @Chalnaayaaar  Digital www.allendigital.in [ 61 ]  TG: @Chalnaayaaar NEET : Biology Exercise - II (Previous Year Questions) AIPMT/NEET Understanding AIPMT 2009 8. A transgenic food crop which may help in 1. Two microbes found to be very useful in solving the problem of night blindness in genetic engineering are :– developing countries is :- (1) Escherichia coli and Agrobacterium (1) Starlink maize tumefaciens (2) Bt Soybean (2) Vibrio cholerae and a tailed bacteriophage (3) Golden rice (3) Diplococcus sp. and Pseudomonas sp. (4) Flavr Savr tomatoes (4) Crown gall bacterium and Caenorhabditis AIPMT 2009 elegans 2. Restriction endonuclease :– 9. Which one of the following is commonly used (1) Cuts the DNA molecule randomly in transfer of foreign DNA into crop plants ? (2) Cuts the DNA molecule at specific sites (1) Penicillium expansum (3) Restricts the synthesis of DNA inside the (2) Trichoderma harzianum nucleus (3) Meloidogyme incognita (4) Synthesizes DNA (4) Agrobacterium tumefaciens 3. Golden rice is a promising transgenic crop. 10. Polyethylene glycol method is used for :- When released for cultivation, it will help in:- (1) Energy production from sewage (1) Alleviation of vitamin A deficiency (2) Gene transfer without a vector (2) Pest resistance (3) Biodiesel production (3) Herbicide tolerance (4) Seedless fruit production (4) Producing a petrol-like fuel from rice 11. Transgenic plants are the ones :- (1) Grown in artificial medium after AIPMT 2007 hybridization in the field 4. A genetically engineered micro-organism (2) Produced by a somatic embryo in artificial used successfully in bioremediation of oil medium spills is a species of :- (3) Generated by introducing foreign DNA in (1) Pseudomonas (2) Trichoderma to a cell and regenerating a plant from (3) Xanthomonas (4) Bacillus that cell (4) Produced after protoplast fusion in AIPMT 2008 artificial medium 5. Cry1 endotoxins obtained from Bacillus 12. The bacterium Bacillus thuringiensis is widely thuringiensis are effective against :- used in contemporary biology as :- (1) Flies (2) Nematodes (1) Source of industrial enzyme (3) Boll worms (4) Mosquitoes (2) Indicator of water pollution 6. Human insulin is being commercially produced from a transgenic species of :- (3) Insecticide (1) Mycobacterium (2) Rhizobium (4) Agent for production of dairy products (3) Saccharomyces (4) Escherichia 13. What is true about Bt toxin ? 7. Main objective of production/use of (1) The concerned Bacillus has antitoxins herbicide resistant GM crops is to :- (2) The inactive protoxin gets converted into (1) Eliminate weeds from the field without active form in the insect gut the use of herbicides (3) Bt protein exists as active toxin in the (2) Encourage eco-friendly herbicides Bacillus (3) Reduce herbicide accumulation in food (4) The activated toxin enters the ovaries of articles for health safety (4) Eliminate weeds from the field without the pest to sterilise it and thus prevent its the use of manual labour multiplication TG: @Chalnaayaaar [ 62 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 14. The genetic defect adenosine deaminase (ADA) 19. An improved variety of transgenic basmati deficiency may be cured permanently by: rice :- (1) Enzyme replacement therapy (1) is completely resistant to all insect pests (2) Periodic infusion of genetically engineered and diseases of paddy lymphocytes having functional ADA cDNA (2) gives high yield but has no charactristic (3) Administering adenosine deaminase aroma activators (3) does not require chemical fertilizers and (4) Introducing bone marrow cells producing growth hormones ADA into cells at early embryonic stages (4) give high yield and is rich in vitamin A 20. Genetic engineering has been successfuly used for producing :- AIPMT 2010 (1) transgenic Cow-Roise which produces 15. Which one of the following is used as vector high fat milk for making ghee for cloning genes into higher organisms? (2) animals like bulls for farm work as they (1) Rhizopus nigricans have super power (2) Retrovirus (3) transgenic mice for testing safety of polio (3) Baculovirus vaccine before use in humans (4) Salmonella typhimurium (4) transgenic models for studying new 16. Restriction endonucleases are enzymes treatments for certain cardiac diseases which :- 21. The genetically-modified (GM) brinjal in (1) restrict the action of the enzyme DNA India has been developed for :- (1) Enhancing mineral content polymerase (2) Drought-resistance (2) remove nucleotides from the ends of the (3) Insect-resistance DNA molecule (4) Enhancing shelf life (3) make cuts at specific positions within the 22. Some of the characteristics of Bt cotton are:- DNA molecule (1) High yield and production of toxic protein (4) recognize a specific nucleotide sequence crystals which kill dipteran pests for binding of DNA ligase (2) High yield and resistance to bollworms 17. Which one of the following palindromic base (3) Long fibre and resistance to aphids sequences in DNA can be easily cut at about (4) Medium yield, long fibre and resistance to the middle by some particular restriction beetle pests enzyme? AIPMT Pre 2011 (1) 5'_______GAATTC ________3' 23. Given below is a sample of a portion of DNA 3'_______ CTTAAG ________5' strand giving the base sequence on the (2) 5'_______CACGTA ________3' opposite strands. What is so special shown in it? 3'_______ CTCAGT________5' 5'______GAATTC_______3' (3) 5'_______CGTTCG ________3' 3'______CTTAAG_______5' 3'_______ ATGGTA _______5' (1) Replication completed (4) 5'_______GATATG ________3' (2) Delection mutation 3'_______ CTACTA ________5' (3) Start codon at the 5' end 18. DNA or RNA segment tagged with a radiactive (4) Palindromic sequence of base pairs molecule is called:- 24. There is a restriction endonuclease called Eco (1) Clone (2) Plasmid RI. What does "co" part in it stand for ? (1) Colon (2) Coelom (3) Vector (4) Probe (3) Coenzyme (4) Coli TG: @Chalnaayaaar  Digital www.allendigital.in [ 63 ]  TG: @Chalnaayaaar NEET : Biology 25. Agarose extracted from sea weeds finds use in: 31. Which one of the following techniques made (1) Spectrophotometry (2) Tissue Culture it possible to genetically engineer living (3) PCR (4) Gel electrophoresis organisms? 26. Maximum number of existing transgenic (1) Hybridization animals is of :- (2) Recombinant DNA techniques (1) Fish (2) Mice (3) Cow (4) Pig (3) X-ray diffraction 27. The process of RNA interference has been (4) Heavier isotope labelling used in the development of plants resistant to: AIPMT Pre 2012 (1) Nematodes (2) Fungi 32. Which one is a true statement regarding DNA (3) Viruses (4) Insects polymerase used in PCR ? AIPMT Mains 2011 (1) It is isolated from a virus (2) It remains active at high temperature 28. Read the following four statements (A-D) (3) It is used to ligate introduced DNA in about certain mistakes in two of them:- recipient cells (A) The first transgenic buffalo, Rosie (4) It serves as a selectable marker produced milk which was human alpha- 33. For transformation, micro-particles coated lactalbumin enriched. with DNA to be bombarded with gene gun are (B) Restriction enzymes are used in isolation made up of :- of DNA from other macro molecules. (1) Silicon or Platinum (2) Gold or Tungsten (C) Downstream processing is one of the (3) Silver or platinum (4) Platinum or zinc steps of R-DNA technology. 34. The figure below is the diagrammatic (D) Disarmed pathogen vectors are also used representation of the E. coli vector pBR322. in transfer of R-DNA into the host. Which one of the given options correctly identifies its certain component(s)? Which are the two statements having Cla I Hind III mistakes ? EcoR I (1) Statements (A) and (B) Pvu I BamH I (2) Statements (B) and (C) Pst I ampR tetR (3) Statements (C) and (D) PBR322 Sal I (4) Statements (A) and (C) ori rop 29. Silencing of mRNA has been used in producing transgenic plants resistant to:- (1) Bacterial blights (2) Bollworms Pvu II (3) Nematodes (4) White rusts (1) Hind III, Eco RI-selectable markers 30. Bacillus thuringiensis forms protein crystals (2) ampR, tetR-antibiotic resistance genes which contain insecticidal protein. (3) ori-original restriction enzyme This protein :- (4) rop-reduced osmotic pressure (1) does not kill the carrier bacterium which 35. Consumption of which one of the following is itself resistant to this toxin foods can prevent the kind of blindness associated with vitamin 'A' deficiency? (2) binds with epithelial cells of midgut of the (1) Golden rice (2) Bt-Brinjal insect pest ultimately killing it (3) Flaver Savr 'tomato (4) Canolla (3) is coded by several genes including the 36. DNA or RNA segment tagged with a radiactive gene cry molecule is called :- (4) is activated by acid pH of the foregut of (1) Clone (2) Plasmid the insect pest (3) Vector (4) Probe TG: @Chalnaayaaar [ 64 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) AIPMT Mains 2012 41. Tobacco plants resistant to a nematode have 37. Which one of the following represents a been developed by the introduction of DNA palindromic sequence in DNA ? that produced (in the host cells) :- (1) an antifeedant 5'- CATTAG -3' 5'- GATACC -3' (1) (2) (2) a toxic protein 3'- GATAAC -5' 3'- CCTAAG -5' (3) both sense and anti-sense RNA 5'- GAATTC -3' 5'- CCAATG -3' (4) a particular hormone (3) (4) 3'- CTTAAG -5' 3'- GAATCC -5' 42. The first clinical gene therapy was given for 38. In genetic engineering, the antibiotics are treating :- used :- (1) Rheumatoid arthritis (1) as sequences from where replication starts (2) Adenosine deaminase deficiency (2) to keep the cultures free of infection (3) Diabetes mellitus (3) as selectable markers (4) Chicken pox (4) to select healthy vectors NEET(UG) 2013 39. The figure below shows three steps (A,B, C) of Polymerase Chain Reaction (PCR). Select the 43. The colonies of recombinant bacteria appear option giving correct identification together white in contrast to blue colonies of non- with what it represents? recombinant bacteria because of :- (1) Inactivation of glycosidase enzyme in Region to be amplified 5' 3' recombinant bacteria A 3' dsDNA (2) Non-recombinant bacteria containing 5' beta-galactosidase 5' 3' (3) Insertional inactivation of alpha- 3' 5' galactosidase in non-recombinant bacteria B 5' 3' (4) Insertional inactivation of beta- 3' 5' galactosidase in recombinant bacteria 44. DNA fragments generated by the restriction 5' 3' endonucleases in a chemical reaction can be C 3' 5' separated by :- 5' 3' (1) Restriction mapping 3' 5' (2) Centrifugation (3) Polymerase chain reaction (1) C-Extension in the presence of heat stable (4) Electrophoresis DNA polymerase (2) A-Annealing with two sets of primers AIPMT 2014 (3) B-Denaturation at a temperature of about 45. An analysis of chromosomal DNA using the 98°C separating the two DNA strands Southern hybridization technique does not use: (4) A-Denaturation at a temperature of about (1) Electrophoresis (2) Blotting 50°C (3) Autoradiography (4) PCR 40. Biolistics (gene-gun) is suitable for :- 46. Which vector can clone only a small fragment (1) Constructing recombinant DNA by joining of DNA? with vectors (1) Bacterial artificial chromosome (2) DNA finger printing (2) Yeast artificial chromosome (3) Disarming pathogen vectors (3) Plasmid (4) Transformation of plants cells (4) Cosmid TG: @Chalnaayaaar  Digital www.allendigital.in [ 65 ]  TG: @Chalnaayaaar NEET : Biology 47. The first human hormone produced by 54. The introduction of T-DNA into plants recombinant DNA technology is :- involves: - (1) Insulin (2) Estrogen (1) Allowing the plant roots to stand in water (3) Thyroxin (4) Progesterone (2) Infection of the plant by Agrobacterium tumefaciens AIPMT 2015 (3) Altering the pH of the soil, then heat 48. The crops engineered for glyphosate are shocking the plants resistant/tolerant to :- (4) Exposing the plants to cold for a brief (1) Bacteria (2) Insects period (3) Herbicides (4) Fungi 49. In Bt cotton, the Bt toxin present in plant NEET-I 2016 tissue as pro-toxin is converted into active 55. The two polypeptides of human insulin are toxin due to:- linked together by :- (1) Acidic pH of the insect gut (1) Hydrogen bonds (2) Action of gut micro-organisms (2) Phosphodiester bond (3) Presence of conversion factors in insect gut (3) Covalent bond (4) Alkaline pH of the insect gut (4) Disulphide bridges 50. Which body of the Government of India 56. Which of the following is not a feature of the regulates GM research and safety of plasmids ? introducing GM organisms for public (1) Independent replication services? (2) Circular structure (1) Indian Council of Agricultural Research (3) Transferable (2) Genetic Engineering Approval Committee (4) Single - stranded (3) Research Committee on Genetic 57. The taq polymerase enzyme is obtained Manipulation from:- (4) Bio-safety committee (1) Thermus aquaticus (2) Thiobacillus ferroxidans Re-AIPMT 2015 (3) Bacillus subtilis 51. The DNA molecules to which the gene of (4) Pseudomonas putida interest is integrated for cloning is called :- 58. Which of the following is a restriction (1) Carrier (2) Transformer endonuclease? (3) Vector (4) Template (1) Hind II (2) Protease 52. The cutting of DNA at specific locations (3) DNase I (4) RNase became possible with the discovery of :- NEET-II 2016 (1) Ligases (2) Restriction enzymes 59. Stirred-tank bioreactors have been designed (3) Probes for :- (4) Selectable markers (1) availability of oxygen throughout the 53. Golden rice is a genetically modified crop process plant where the incorporated gene is meant (2) ensuring anaerobic conditions in the for biosynthesis of :- culture vessel (1) Vitamin A (2) Vitamin B (3) purification of product (3) Vitamin C (4) Omega 3 (4) addition of preservatives to the product TG: @Chalnaayaaar [ 66 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 60. A foreign DNA and plasmid cut by the same 68. In India, the organisation responsible for restriction endonuclease can be joined to assessing the safety of introducing genetically form a recombinant plasmid using :- modified organisms for public use is :- (1) Polymerase-III (2) Ligase (1) Indian Council of Medical Research (3) Eco RI (4) Taq polymerase (ICMR) 61. Which of the following is not a component of (2) Council for Scientific and Industrial downstream processing ? Research (CSIR) (1) Preservation (2) Expression (3) Research Committee on Genetic (3) Separation (4) Purification Manipulation (RCGM) 62. Which of the following restriction enzymes (4) Genetic Engineering Appraisal Committee produces blunt ends ? (GEAC) (1) Xho I (2) Hind III 69. A 'new variety of rice was patented by a (3) Sal I (4) Eco RV foreign company though such varieties have 63. Which kind of therapy was given in 1990 to a been present in India for a long time. This is four year old girl with adenosine deaminase related to :- (ADA) deficiency ? (1) Co-667 (2) Sharbati Sonora (1) Immunotherapy (2) Radiation therapy (3) Lerma Rojo (4) Basmati (3) Gene therapy (4) Chemotherapy 70. Use of bioresources by multinational companies and organisations without NEET(UG) 2017 authorisation from the concerned country 64. The DNA fragments separated on an agarose and its people is called :- gel can be visualised after staining with :- (1) Bio-infringement (1) Acetocarmine (2) Biopiracy (2) Aniline blue (3) Biodegradation (3) Ethidium bromide (4) Bioexploitation (4) Bromophenol blue 71. The correct order of steps in Polymerase 65. The process of separation and purification of Chain Reaction (PCR) is :- expressed protein before marketing is called: (1) Extension, Denaturation, Annealing (1) Downstream processing (2) Annealing, Extension, Denaturation (2) Bioprocessing (3) Denaturation, Extension, Annealing (3) Postproduction processing (4) Denaturation, Annealing, Extension (4) Upstream processing 66. A gene whose expression helps to identify NEET(UG) 2019 transformed cell is known as :- 72. Which of the following is true for Golden (1) Vector (2) Plasmid rice? (3) Structural gene (4) Selectable marker (1) It is Vitamin A enriched, with a gene from daffodil NEET(UG) 2018 (2) It is pest resistant, with a gene from 67. Which of the following is commonly used as a Bacillus thuringiensis vector for introducing a DNA fragment in (3) It is drought tolerant, developed using human lymphocytes ? Agrobacterium vector (1) Retrovirus (2) Ti plasmid (4) It has yellow grains, because of a gene (3)  phage (4) pBR322 introduced from a primitive variety of rice TG: @Chalnaayaaar  Digital www.allendigital.in [ 67 ]  TG: @Chalnaayaaar NEET : Biology 73. Which of the following features of genetic 78. The two antibiotic resistance genes on vector code does allow bacteria to produce human pBR322 are :- insulin by recombinant DNA technology? (1) Ampicillin and Tetracycline (1) Genetic code is not ambiguous (2) Ampicillin and Chloramphenicol (2) Genetic code is redundant (3) Chloramphenicol and Tetracycline (3) Genetic code is nearly universal (4) Tetracycline and Kanamycin (4) Genetic code is specific 79. Exploitation of bioresources of a nation by 74. Following statements describe the multinational companies without characteristics of the enzyme Restriction authorization from the concerned country is endonuclease. Identify the incorrect statement: referred to as: - (1) The enzyme cuts DNA molecule at (1) Bioweapon (2) Biopiracy identified position within the DNA (3) Bioethics (4) Biowar (2) The enzyme binds DNA at specific sites 80. A selectable marker is used to: - and cuts only one of the two strands. (1) help in eliminating the non-transformants, (3) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand. so that the transformants can be (4) The enzyme recognizes a specific regenerated palindromic nucleotide sequence in the DNA (2) identify the gene for a desired trait in an 75. DNA precipitation out of a mixture of alien organism biomolecules can be achieved by treatment (3) select a suitable vector for transformation with :- in a specific crop (1) Isopropanol (4) mark a gene on a chromosome for (2) Chilled ethanol isolation using restriction enzyme (3) Methanol at room temperature 81. Given below are four statements pertaining (4) Chilled chloroform to separation of DNA fragments using gel 76. What triggers activation of protoxin to active electrophoresis. Identify the incorrect Bt toxin of Bacillus thuringiensis in boll worm? statements: - (1) Body temperature (a) DNA is negatively charged molecule and (2) Moist surface of midgut so it is loaded on gel towards the Anode (3) Alkaline pH of gut terminal (4) Acidic pH of stomach (b) DNA fragments travel along the surface of the gel whose concentration does not NEET(UG) 2019 (Odisha) affect movement of DNA. 77. Match the following enzymes with their (c) Smaller the size of DNA fragment larger is functions:- the distance it travels through it. (a) Restriction (i) Joins the DNA (d) Pure DNA can be visualized directly by endonuclease fragements exposing UV radiation. (b) Restriction (ii) Extends primers Choose correct answer from the options exonuclease on genomic given below (1) (a), (c) and (d) (2) (a), (b) and (c) DNA template (3) (b), (c) and (d) (4) (a), (b) and (d) (c) DNA ligase (iii) Cuts DNA at specific position 82. An enzyme catalysing the removal of (d) Taq polymerase (iv) Removes nucleotides from ends of DNA is:- nucleotides from (1) DNA ligase (2) Endonuclease the ends of DNA (3) Exonuclease (4) Protease Select the correct option from the following: - 83. In RNAi, the genes are silenced using:- (1) a-iii, b-i, c-iv d-ii (2) a-iii, b-iv, c-i, d-ii (1) ds-RNA (2) ss-DNA (3) a-iv, b-iii, c-i, d-ii (4) a-ii, b-iv, c-i, d-iii (3) ss-RNA (4) ds-DNA TG: @Chalnaayaaar [ 68 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) NEET(UG) 2020 89. Match the following columns and select the 84. Which of the following statements is not correct option:- correct? Column - I Column - II (1) Genetically engineered insulin is (a) Bt cotton (i) Gene therapy produced in E coli. (b) Adenosine (ii) Cellular defence (2) In man insulin is synthesised as a deaminase deficiency proinsulin. (c) RNAi (iii) Detection of HIV (3) The proinsulin has an extra peptide called infection C-peptide. (d) PCR (iv) Bacillus (4) The functional insulin has A and B chains thuringiensis linked together by hydrogen bonds. (a) (b) (c) (d) 85. Match the organism with its use in (1) (i) (ii) (iii) (iv) biotechnology. (2) (iv) (i) (ii) (iii) (a) Bacillus (i) Cloning vector (3) (iii) (ii) (i) (iv) thuringiensis (4) (ii) (iii) (iv) (i) (b) Thermus (ii) Construction of 90. Identify the wrong statement with regard to aquaticus first rDNA molecule Restriction Enzymes. (c) Agrobacterium (iii) DNA polymerase (1) Sticky ends can be joined by using DNA tumefaciens ligases. (d) Salmonella (iv) Cry proteins (2) Each restriction enzyme functions by typhimurium inspecting the length of a DNA sequence. Select the correct option from the following:- (3) They cut the strand of DNA at palindromic (a) (b) (c) (d) sites. (1) (iii) (iv) (i) (ii) (4) They are useful in genetic engineering. (2) (ii) (iv) (iii) (i) 91. The sequence that controls the copy number (3) (iv) (iii) (i) (ii) of the linked DNA in the vector, is termed :- (4) (iii) (ii) (iv) (i) (1) Recognition site (2) Selectable marker 86. Bt cotton variety that was developed by the (3) Ori site introduction of toxin gene of Bacillus (4) Palindromic sequence thuringiensis (Bt) is resistant to :- (1) Insect predators (2) Insect pests NEET(UG) 2020 (COVID-19) (3) Fungal diseases (4) Plant nematodes 92. First discovered restriction endonuclease 87. The specific palindromic sequence which is that always cuts DNA molecule at a particular recognized by EcoR I is :- point by recognising a specific sequence of six (1) 5' - GGATCC - 3' , 3' - CCTAGG - 5' base pairs is:- (2) 5' - GAATTC - 3' , 3' - CTTAAG - 5' (1) EcoR1 (3) 5' - GGAACC - 3' , 3' - CCTTGG - 5' (2) Adenosine deaminase (4) 5' - CTTAAG - 3', 3' - GAATTC - 5' (3) Thermostable DNA polymerase 88. Choose the correct pair from the following:- (4) Hind II 93. In Recombinant DNA technology antibiotics (1) Exonucleases: - Make cuts at specific are used :- positions within DNA (1) to keep medium bacteria-free (2) Ligases :- Join the two DNA molecules (2) to detect alien DNA (3) Polymerases: Break the DNA into fragments (3) to impart disease-resistance to the host plant (4) Nucleases : Separate the two strands of DNA (4) as selectable markers TG: @Chalnaayaaar  Digital www.allendigital.in [ 69 ]  TG: @Chalnaayaaar NEET : Biology 94. Match the following techniques or 98. RNA interference is used for which of the instruments with their usage :- following purposes in the field of (a) Bioreactor (i) Separation of biotechnology ? DNA fragments (1) to develop a plant tolerant to abiotic (b) Electrophoresis (ii) Production of large stresses quantities of (2) to develop a pest resistant plant against products infestation by nematode (c) PCR (iii) Detection of (3) to enhance the mineral usage by the plant pathogen, based (4) to reduce post-harvest losses on antigen – 99. The laws and rules to prevent unauthorised antibody reaction exploitation of bio-resources are termed as- (d) ELISA (iv) Amplification of (1) Biopatenting nucleic acids (2) Bioethics Select the correct option from following:- (3) Bioengineering (1) (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) (4) Biopiracy (2) (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) (3) (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) NEET(UG) 2021 (4) (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) 100. During the purification process for 95. In a mixture, DNA fragments are separated recombinant DNA technology, addition of by:- chilled ethanol precipitates out:- (1) Bioprocess engineering (1) RNA (2) Restriction digestion (2) DNA (3) Electrophoresis (3) Histones (4) Polymerase chain reaction (4) Polysaccharides 96. Spooling is :- 101. Which of the following is a correct sequence (1) Amplification of DNA of steps in a PCR (Polymerase Chain (2) Cutting of separated DNA bands from the Reaction) ? agarose gel (1) Denaturation, Annealing, Extension (3) Transfer of separated DNA fragments to (2) Denaturation, Extension, Annealing synthetic membranes (3) Extension, Denaturation, Annealing (4) Collection of isolated DNA (4) Annealing, Denaturation, Extension 97. Select the correct statement from the 102. DNA strands on a gel stained with ethidium following :- bromide when viewed under UV radiation, (1) Gel electrophoresis is used for appear as :- amplification of a DNA segment. (1) Yellow bands (2) The polymerase enzyme joins the gene of (2) Bright orange bands interest and the vector DNA. (3) Dark red bands (3) Restriction enzyme digestions are (4) Bright blue bands performed by incubating purified DNA 103. When gene targetting involving gene molecules with the restriction enzymes of amplification is attempted in an individual's optimum conditions. tissue to treat disease, it is known as :- (4) PCR is used for isolation and separation of (1) Biopiracy (2) Gene therapy gene of interest. (3) Molecular diagnosis (4) Safety testing TG: @Chalnaayaaar [ 70 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 104. Which of the following is not an application 109. For effective treatment of the disease, early of PCR (Polymerase Chain Reaction) ? diagnosis and understanding its (1) Molecular diagnosis pathophysiology is very important. Which of the following molecular diagnostic techniques (2) Gene amplification is very useful for early detection? (3) Purification of isolated protein (1) Western Blotting Technique (4) Detection of gene mutation (2) Southern Blotting Technique 105. Plasmid pBR322 has PstI restriction enzyme (3) ELISA Technique site within gene ampR that confers ampicillin (4) Hybridization Technique resistance. If this enzyme is used for inserting a gene for -galactoside production and the NEET(UG) 2022 recombinant plasmid is inserted in an E.coli 110. Which one of the following statement is not strain true regarding gel electrophoresis (1) it will not be able to confer ampicillin technique? resistance to the host cell. (1) The separated DNA fragments are (2) the transformed cells will have the ability stained by using ethidium bromide. to resist ampicillin as well as produce - (2) The presence of chromogenic substrate galactoside. gives blue coloured DNA bands on the gel. (3) it will lead to lysis of host cell. (3) Bright orange coloured bands of DNA (4) it will be able to produce a novel protein can be observed in the gel when with dual ability. exposed to UV light. 106. A specific recognition sequence identified by (4) The process of extraction of separated endonucleases to make cuts at specific DNA strands from gel is called elution. positions within the DNA is :- 111. Given below are two statements :- (1) Degenerate primer sequence one is labelled as Assertion (A) and the (2) Okazaki sequences other is labelled as Reason (R). (3) Palindromic Nucleotide sequences Assertion (A) : Polymerase chain reaction (4) Poly(A) tail sequences is used in DNA amplification 107. With regard to insulin choose correct options. Reason (R) : The ampicillin resistant gene (a) C-peptide is not present in mature is used as a selectable marker to check insulin. transformation. (b) The insulin produced by rDNA In the light of the above statements, choose technology has C-peptide. the correct answer from the options given (c) The pro-insulin has C-peptide. below:- (d) A-peptide and B-peptide of insulin are (1) Both (A) and (R) are correct but (R) is interconnected by disulphide bridges. not the correct explanation of (A) (2) (A) is correct but (R) is not correct Choose the correct answer from the options (3) (A) is not correct but (R) is correct given below. (4) Both (A) and (R) are correct and (R) is (1) (b) and (d) only (2) (b) and (c) only the correct explanation of (A) (3) (a), (c) and (d) only (4) (a) and (d) only 112. In the following palindromic base sequences 108. During the process of gene amplification of DNA, which one can be cut easily by using PCR, if very high temperature is not particular restriction enzyme ? maintained in the beginning, then which of (1) 5' G A A T T C 3'; 3' C T T A A G 5' the following steps of PCR will be affected first? (2) 5' C T C A G T 3'; 3' G A G T C A 5' (1) Annealing (2) Extension (3) 5' G T A T T C 3'; 3' C A T A A G 5' (3) Denaturation (4) Ligation (4) 5' G A T A C T 3'; 3' C T A T G A 5' TG: @Chalnaayaaar  Digital www.allendigital.in [ 71 ]  TG: @Chalnaayaaar NEET : Biology 113. Given below are two statements:- (e) Some patients develop allergic reactions Statement I : Restriction endonucleases to the foreign insulin. recognise specific sequence to cut DNA Choose the most appropriate answer from known as palindromic nucleotide sequence. the options given below:- Statement II : Restriction endonucleases (1) (b)only cut the DNA strand a little away from the (2) (c) and (d) only centre of the palindromic site. (3) (c), (d) and (e) only In the light of the above statements, choose (4) (a), (b) and (d) only the most appropriate answer from the options given below:- Re-NEET 2022 (1) Both Statement I and Statement II are 117. Separation of DNA, fragments is done by a incorrect technique known as :- (2) Statement I is correct but Statement II (1) Polymerase Chain Reaction is incorrect (2) Recombinant technology (3) Statement I is incorrect but Statement (3) Southern blotting II is correct (4) Gel electrophoresis (4) Both Statement I and Statement II are 118. Match List-I with List-II :- correct List-I List-II 114. Which of the following is not a desirable (a) Gene gun (i) Replacement of a feature of a cloning vector ? faulty gene by a (1) Presence of a marker gene normal healthy gene (2) Presence of single restriction enzyme site (b) Gene therapy (ii) Used for transfer of (3) Presence of two or more recognition sites gene (4) Presence of origin of replication (c) Gene cloning (iii) Total DNA in the cells 115. Transposons can be used during which one of an organism of the following? (d) Genome (iv) To obtain indentical (1) Gene silencing copies of a particular (2) Autoradiography DNA molecule (3) Gene sequencing Choose the correct answer from the (4) Polymerase Chain Reaction options given below :- 116. Statements related to human Insulin are (1) (a) - (ii), (b) - (i), (c) - (iv), (d) - (iii) given below :- (2) (a) - (i), (b) - (iii), (c) - (ii), (d) - (iv) Which statement(s) is/ are correct about (3) (a) - (iv), (b) - (i), (c) - (iii), (d) - (ii) genetically engineered Insulin ? (4) (a) - (ii), (b) - (iii), (c) - (iv), (d) - (i) (a) Pro-hormone insulin contain extra 119. Pathogenic bacteria gain resistance to stretch of C-peptide antibiotics due to changes in their :- (b) A-peptide and B-peptide chains of (1) Cosmids (2) Plasmids insulin were produced separately in (3) Nucleus (4) Nucleoid E.coli, extracted and combined by 120. Which of the following methods is not creating disulphide bond between commonly used for introducing foreign them. (c) Insulin used for treating Diabetes was DNA into the plant cell? extracted from Cattles and Pigs. (1) Agrobacterium mediated transformation (d) Pro-hormone Insulin needs to be (2) Gene gun processed for converting into a mature (3) 'Disarmed pathogen' vectors and functional hormone. (4) Bacteriophages TG: @Chalnaayaaar [ 72 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 121. Refer to the following statements for (1) (a) and (b) only agarose-gel electrophoresis :- (2) (a), (b) and (c) only (a) Agarose is a natural polymer obtained (3) (a), (b) and (d) only from seaweed. (4) (b), (c) and (d) only (b) The separation of DNA molecules in 122. Milk of transgenic 'Cow Rosie' was agarose-gel electrophoresis depends on nutritionally more balanced product for the size of DNA. human babies than natural cow milk (c) The DNA migrates from negatively- because it contained :- charged electrode to the positively- (1) Human protein –1–antitrypsin charged electrode (2) Human alpha–lactalbumin (d) The DNA migrates from positively- (3) Human insulin–like growth factor charged electrode to the negatively- (4) Human enzyme Adenosine Deaminase charged electrode. (ADA) Choose the most appropriate answer from the options given below :- EXERCISE-II (Previous Year Questions) ANSWER KEY Question 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Answer 1 2 1 1 3 4 4 3 4 2 3 3 2 4 2 Question 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Answer 3 1 4 4 3 3 2 4 4 4 2 1 1 3 2 Question 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 Answer 2 2 2 2 1 4 3 3 1 4 3 2 4 4 4 Question 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Answer 3 1 3 4 2 3 2 1 2 4 4 1 1 1 2 Question 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 Answer 2 4 3 3 1 4 1 4 4 2 4 1 3 2 2 Question 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 Answer 3 2 1 2 1 4 3 1 4 3 2 2 2 2 1 Question 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 Answer 3 4 4 2 3 4 3 2 1 2 1 2 2 3 1 Question 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 Answer 3 3 3 3 2 1 1 4 3 1 1 4 1 2 4 Question 121 122 Answer 2 2 TG: @Chalnaayaaar  Digital www.allendigital.in [ 73 ]  TG: @Chalnaayaaar NEET : Biology Exercise - III Master Your Understanding EXERCISE-III(A) NCERT BASED QUESTIONS 8. To isolate DNA from the plant cells we have to 1. The science, which deals with techniques of break the wall this is done by :- using live organisms or enzymes from (1) Lysozyme (2) Cellulase organism to produce products and processes (3) Chitinase (4) Invertase useful to human is :- 9. Restriction enzymes belong to a larger class (1) Genetics (2) Biotechnology of enzymes called :- (3) Bioinformatics (4) None of these (1) Cellulases (2) Hydrolases 2. A restriction endonucleases which always cut (3) Polymerases (4) Nucleases DNA molecules at a particular point by 10. The construction of the first recombinant recognising a specific sequence of six base DNA was done by ? pairs is :- (1) Stanley cohen and Herbert Boyer (1) Hind II (2) Pst I (2) Nathan's and Smith (3) Hae III (4) All of these (3) Maeselson and Stahl 3. The first letter of the name of Restriction (4) Allec Jeffreys endonuclease came from the:- 11. EcoR I recognises palindromic sequence:- (1) Genus of organism (1) 5'GGGCCC3' 3'CCCGGG (2) Species of organism (3) Family of organism (2) 5'-GAATTC-3' 3-CTTAAG-5' (4) Class of organism 4. Autonomously replicating circular extra (3) 5-AAGCTT3' 3-TTCGAA-5 chromosomal DNA of bacteria is :- (1) Plastid (2) Nucleus (4) None of the above (3) Plasmid (4) None of these 12. The enzymes responsible for restricting the 5. The specific DNA sequence in a chromosome growth of bacteriophage in E coli were which is responsible for initiation of isolated in 1963, these enzyme are :- replication is :- (1) DNA ligases (1) Cloning region (2) Alkaline phosphatases (2) Termination region (3) DNA polymerases (3) Initiation region (4) Restriction endonuclease (4) Origin of replication 13. Vector which is commonly used to transfer 6. Which of the following enzymes is known as foreign gene in a crop plant is :- 'genetic glue'? (1) Plasmids of Salmonella (1) DNA polymerase (2)  bacterio phage vector (2) Alkaline phosphatase (3) Ti plasmid of Agrobacterium tumifaciens (3) DNA ligase (4) None of the above (4) All of the above 14. Father of genetic engineering is :- 7. pBR322 has two antibiotic resistance genes, (1) Paul Berg (2) Nathans they are :- (3) Herbert Boyer (4) Stanley Cohen (1) Streptomycin and Ampicillin resistant gene 15. A definition of biotechnology that encompasses (2) Chloromycetin and tetracycline resistant both traditional view and modern view are gene given by :- (3) Tetracycline and neomycin resistant (1) European forum on Biotechnology genes (2) European focus on Biotechnology (4) Ampicillin and tetracyclin resistant genes (3) European Federation of Biotechnology (4) European Centre of Biotechnology TG: @Chalnaayaaar [ 74 ] www.allendigital.in  Digital  TG: @Chalnaayaaar Biotechnology : (I & II) 16. Which one of the following is must in Genetic 25. The enzymes, which remove nucleotides engineering? from the ends of the DNA are :- (1) Restriction endonuclease + DNA ligase (1) Exonuclease (2) Endonuclease (2) Restriction exonuclease + DNA polymerase (3) Cellulase (4) Hydrolase (3) Alkaline phosphate + DNA Ligase 26. Group of letters that form the same words (4) RNA polymerase + DNA polymerase when read both forward and backward is 17. Roman numbers following the names of called :- restriction endonuclease indicate :- (1) Palindrome (2) Same words (1) The order in which the enzymes were (3) Opposite words (4) None of the above isolated from that strain of bacteria 27. Which type of ends are produced by EcoR I? (2) strain of bacteria (1) Blunt ends (2) Sticky ends (3) the order in which genus is taken to (3) Both (1) and (2) (4) None of the above isolate the enzyme 28. The sequence which is responsible for (4) none of the above controlling the copy number of the linked 18. Exonuclease removes nucleotides from:- DNA is :- (1) Specific positions (1) Coding sequence (2) the ends of the DNA (2) Promoter sequence (3) any where in DNA (3) Terminator sequence (4) All the above (4) Ori 19. In a chromosome there is a specific DNA 29. Apart from DNA in the bacterial nucleoid, sequence which is responsible for initiating there is a circular extrachromosomal DNA in replication is :- a bacterial cell called :- (1) Ori (1) Plasmid (2) Palindromic sequence (2) Mesosomes (2) Initiation sequence (3) Chromosome (4) Promoter sequence (4) None of these 20. First recombinant DNA was made by Stanley 30. The stickiness of the ends, facilitates the Cohen and Herbert Boyer in :- action of enzyme :- (1) 1968 (2) 1970 (3) 1972 (4) 1974 (1) DNA ligase 21. The first restriction endonuclease discovered (2) DNA polymerase was:- (3) Alkaline phosphatase (1) EcoR I (2) Sam I (4) All of the above (3) BamH I (4) Hind II 31. Two enzymes responsible for restricting the 22. In the vector pBR322 there is :- growth of bacteriophage in E. coli were (1) One selectable marker isolated in 1963, one of these cut DNA, while (2) Two selectable markers other :- (3) Three selectable markers (1) Add propyl group to DNA (4) None of the above (2)