Protein Sorting and Glycosylation PDF

ead topic sub topic terms amples ther Protein sorting Import 1 Export ynthesis of proteins begins on ribosomes have strtinsgay.ges EYeseygnecess.ir igisnEi Fats E...

ead topic sub topic terms amples ther Protein sorting Import 1 Export ynthesis of proteins begins on ribosomes have strtinsgay.ges EYeseygnecess.ir igisnEi Fats E sign fdtfratsaphoffqtucte.at fates PETbetween cytosol and EE.fm ieiciieetan ptort energy requierment protein at Imm protein translocators another way to sort translocate transmembrane protein ZOOMED IN that transes the membrane In NUCLEAR PORE will transport a protein across the membrane has to unfold like a snakethrough the translocator to get faffial translocation Wr translocation Hse get protein becomes embeded in membrane vesicular transport uses vesicles pinch bud off a membrane it  nucleus and goingtegmen nner nuclear membrane relapse safferent proteins nucleoporins year Yeah Prehidhinin lamina lamina provides the nuclear sorting signal strutural support nuclear localization ammaces oter nuclear membrane continous with E R rt sequences that are rich hat is gettingtransported in positively charged amino acids is tones DNA polymerase ten reg lysine and argenine be found anywhere on amino 49 reg.ggf9spy import receptors port and import 1T End to ran it ofenzyme neqqsanote.intrerbYna to gate.isEcant.EIse activating 0 0 0 trigger GTPhydrolysis losingthe Signals EE E anin exchange GDP off and bring in a GTP so you can import make ranGTDthat goes out in and needs into cytosol joyiermade quiet 8984 19 Feiafg.twfhe TesNLs go into nucleus reach the nuclear sidewhere 8at Iain's w Eause receptor to release o cargo QEMPtkai.me receptor 490 191.25 91 The tainson from the receptor 0  the oooooddk.to f AT will promotecargo to bind to exportreceptors t promotes formation move to cytosolicside engq.gsEFPTEDP nuc I I receptor will release cargo and Ran GDP I ptodnYyiMPyfotplas'mort Plinths with nyganar.GE cargo more GTP due to NES nuclear exportsystem moves to cytosolic side from in nucleus gradient 297 5 complex joins with Ran GAP and hydrolyzes GTP GDP ot causes confirmational 9 91 919,4 p bound change GEF guaninenucleotideexchange Father Ews APhelp's tf Gtpase to hydrolyze the EEmtoles.tt osphat import vclear 1 docking importer proteins 2 NL's aidPatto form complex 3 kanih.tt B9dto importer in nucleus 4 bindingcausesreleaseof cargo 5 RanGTPgoes back to nucleus with import and goes into RanGAP due tohydrolysis head topic sub topic terms examples key info other glycosylation and UPR PRunfolded protein response oligosaccharide carbohydrates made ofmultiplemonosaccharides lycosylation the addition of oligosaccharides TWOTYPES OFGLYCOSYLATION to proteins R Ñ sfarÉ ine linked oligosaccaryl transferase particularamino acid adds sugarchains to precursor oligosaccharide proteins sugar oligosaccharides that is transferred 90 glycoproteins proteingappish sugar to the amino NHz group of theasparagine in Asn X Serine or thronine target protein 499 ther amino acid Olichol molecule that anchors precursor oligosaccharide to the Entarget Saragine Target gasparagine rimming or processing of lycosyl groups can occur wlinked n golgi ag.gg target iietfohya8a.toiiroQps mportance of glycosylation proteinfolding1020,3 40 modification is important or proteinsthat are part of EI.hn idateEetEniie the upfection untilfoldedstate won'tneedanymore Fined naa igo'sccnFi.ae's thereads teroids's in EanPae cEEn proteins that are unfolded glucosyltransferasekeep preventingaggregation addingglucoseto oligosacc http t fyinnfg9flgtingX promoteproperfolding thathavelost glucose action molecs WPhhmpffthepif.fr unfolded proteins have PDI promotes disulfide continuousglucosetrimming bigon promoting bonds andaddinguntilfoldedstate width is achieved maintainsthe attractionfor the cats