유전공학 7.pdf - Genetic Engineering Chapter 7
Document Details
Uploaded by ViewableHope2751
Tags
Summary
This document is related to Genetic Engineering, particularly Chapter 7 on Gene Capture. It includes topics such as gene cloning, gene libraries, and screening methods with diagrams. Processes like PCR (Polymerase Chain Reaction) are also explained.
Full Transcript
GENETIC ENGINEERING Chap. 7 Chap.7. Gene capture 2 복제 및 발현 클로닝 벡터에 삽입 형질전환 유전자 분리 (Gene...
GENETIC ENGINEERING Chap. 7 Chap.7. Gene capture 2 복제 및 발현 클로닝 벡터에 삽입 형질전환 유전자 분리 (Gene (Replication and (Insertion into a (Transformation into isolation) expression in the vector) host cells) host) 3 라이브러리 형질전환을 통한 수많은 클론 생성 스크리닝 타겟 유전자를 가진 클론 찾기 유전자의 분리 재조합 DNA 형질전환 라이브러리 스크리닝 (DNA 추출) 생성 (Transformation) 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 예시 Target 유전자: β-actin Forward: 5’-AGAGCTACGAGCTGCCTGAC-3’ Reverse: 5’-AGCACTGTGTTGGCGTACAG-3’ Tm: 약 60°C / GC 함량: 50% 26
