Protein Function (Globular Protein) PDF
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Uploaded by SteadfastEcoArt4504
Duke University
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Summary
This document presents an overview of protein function, focusing on ligand binding and conformational changes. It explains the differences between induced fit and key-and-lock models, along with their advantages. The document also explores equilibrium, dissociation constants, and oxygen transport.
Full Transcript
Chapter 5 Protein (2) Protein function (globular protein) Topic Question 6-ch5) Explain differences of induced fit versus key and lock model. What are the advantages of each model ? Equilibrium = the same no of reactants and products are forming at any given time. Reaction is still on. Two opp...
Chapter 5 Protein (2) Protein function (globular protein) Topic Question 6-ch5) Explain differences of induced fit versus key and lock model. What are the advantages of each model ? Equilibrium = the same no of reactants and products are forming at any given time. Reaction is still on. Two opposite reactions happening but no net movement. Kd = concentration of ligands at which half of proteins are occupied at equilibrium. equilibrium dissociation constant. Higher Ka = Higher affinity. Higher Kd = Lower affinity. Discussion Kd and θ Kd = 4x 2 Kd = 3 x 3 -------- -------- 2 3 Occupied P (%) Occupied P (%) Oxygen transport
