Ferritin and Haemosiderin Presentation PDF

Ferritin and Haemosiderin Gavin Knight  Learning Objectives Upon completion of this session, you should be able to: 1) Understand and utilise the current guideline algorithm for establishing a diagnosis of iron deficiency 2) Explain the properties of ferritin and haemosiderin 3) Evaluate th...

Ferritin and Haemosiderin Gavin Knight  Learning Objectives Upon completion of this session, you should be able to: 1) Understand and utilise the current guideline algorithm for establishing a diagnosis of iron deficiency 2) Explain the properties of ferritin and haemosiderin 3) Evaluate the relative contributions ferritin and haemosiderin make to iron storage and the diagnosis of anaemia 4) Interpret microscopic images associated with Perl’s staining  Recommended tests for investigating iron deficiency Br J Haematol, Volume: 196, Issue: 3, Pages: 523-529, First published: 24 October 2021, DOI: (10.1111/bjh.17900)  WHAT IS FERRITIN? The cytoplasmic pool of soluble Fe2+ (the labile iron pool) must be kept small as it is capable of promoting oxidative damage Ferritin, the major storage form of iron is found in the bone marrow, liver and spleen Composed of apoferritin and iron-phosphate-hydroxide (ferrihydrite) core Apoferritin has intrinsic catalytic activity that oxidises Fe2+ Ferritin is a water-soluble, hollow and spherical protein consists of 24 subunits Up to 4500 atoms of iron can be stored by a single ferritin molecule 1 μg/L of serum ferritin equates to around 8 mg of stored iron Fe2+ is stored on the inside of the ferritin sphere through incorporation into ferrihydrite as Fe3+ The process begins as Fe2+ into the FT cavity through one of 8 existing pores Ferritin and iron deficiency The amount of circulating ferritin parallels concentration of storage iron Ferritin increases in inflammatory states, kidney disease, liver disease and malignancy – it is an acute phase reactant UK guidelines suggest a value of