Effects of Furosemide on Rabbit Urine Amount PDF
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Uploaded by AffectionateVampire2200
Binzhou Medical University
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Summary
This document presents a laboratory procedure for analyzing the effects of furosemide on rabbit urine production. The procedure details experimental materials, methodology, including animal preparation, drug administration, and data recording. It's suitable for undergraduate medical or pharmacology students working on similar research projects.
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Effects of Furosemide on Amount of Rabbit Urine Experimental Materials 1. Animals: rabbits with weight 2~3kg. 2. Drugs: 25% ethyl carbamate (urethane), 1%furosemide, liquid paraffin, normal saline. 3. Instruments: rabbit operating table, surgical apparatus, beaker (100ml), cannula...
Effects of Furosemide on Amount of Rabbit Urine Experimental Materials 1. Animals: rabbits with weight 2~3kg. 2. Drugs: 25% ethyl carbamate (urethane), 1%furosemide, liquid paraffin, normal saline. 3. Instruments: rabbit operating table, surgical apparatus, beaker (100ml), cannula, plastic catheter, syringe. Method and Procedure 1. Measure the weight of the rabbit, Anesthetization Rabbits were weighed and anaesthetized through intravenous injection (marginal vein of the rabbit's ear) of 25% ulatane (4ml/kg). 2. Fixation The rabbit lay supine and was firmly fixed on the operating table. 3. Operation After removing the fur from the abdomen, specifically in the bladder region, make a 4-6 cm incision along the midline of the abdomen. Locate the bladder and identify both ureters at its base. Carefully separate one side of the ureter for approximately 2 cm and ligate it at its proximal end near the bladder. Prior to intubation, fill a catheter with water. Create a small 'V'-shaped opening above the ligation site to insert the plastic catheter into the ureter towards the kidney. Wait until urine begins to flow out, which indicates that the procedure has been successful and that there are no blockages in the catheter. Subsequently, secure the catheter with thread to prevent it from slipping out. Finally, place the distal end of the catheter into a beaker to collect urine and count any drops over a period of 5 minutes. 4. Administration After counting the urine drops for 5 minutes prior to administration, inject 0.5 ml/kg (equivalent to 5 mg/kg) of 1% furosemide into the marginal vein of the rabbit's ear. Subsequently, count the urine drops in 3 consecutive intervals of 5 minutes following administration. Results
