Effects of Furosemide on Rabbit Urine

Effects of Furosemide on Rabbit Urine

• Study investigated furosemide's impact on rabbit urine output.
• Conducted at Binzhou Medical University, Department of Pharmacology.

Experimental Materials

• Animals: Rabbits weighing 2-3 kg.
• Drugs: 25% ethyl carbamate (urethane), 1% furosemide, liquid paraffin, normal saline.
• Instruments: Rabbit operating table, surgical apparatus, 100ml beakers, cannula, plastic catheter, syringe.

Method and Procedure

• Anesthetization: Rabbits were weighed and anesthetized via intravenous injection (marginal vein of the ear) with 25% ulatane (4ml/kg).

• Fixation: Rabbits were securely fixed supine on the operating table.

• Operation:

  • A 4-6 cm incision was made along the midline of the abdomen, focusing on the bladder region.
  • The bladder and ureters were located and identified.
  • One ureter was carefully separated (approximately 2 cm) and ligated at its proximal end.
  • A catheter was filled with water, and a small 'V'-shaped opening was created above the ligation site to insert the catheter into the ureter towards the kidney.
  • Urine flow confirmation indicated successful catheter insertion and no blockages.
  • The catheter was secured with thread to prevent slippage.
  • The distal catheter end was placed in a beaker to collect urine.
  • Urine drops were counted over a 5-minute period.

• Administration:

  • After the initial 5-minute urine collection, 0.5 ml/kg (equivalent to 5 mg/kg) of 1% furosemide was injected into the marginal vein of the rabbit's ear.
  • Urine drops were subsequently counted in 3 consecutive 5-minute intervals following furosemide administration.

Results

• Data collection is required to analyze furosemide's effect on urine output (before and after administration).
• A table was provided to record the results. The table is titled "Table Effect of furosemide on amount of urine".
• The table includes columns for Drug, Dose (mg/kg), Before (administration), and After administration (0-5 min, 5-10 min, 10-15 min).
• The dose of furosemide was 5 mg/kg.

This quiz explores the impact of furosemide on urine output in rabbits based on a study conducted at Binzhou Medical University. It covers experimental methods, materials, and procedures used to administer the drug and analyze its effects on renal function. Dive into the details of pharmacological research in animal models.