Week 1 Lab Microscope PDF

THE MICROSCOPE QUIO M. COM, RMT MICROSCOPE instrument that produces enlarged images of small objects, allowing the observer an exceedingly close view of minute structures at a scale convenient for examination and analysis 1878: A mathematical theory linking resolution to light wavelength is...

THE MICROSCOPE QUIO M. COM, RMT MICROSCOPE instrument that produces enlarged images of small objects, allowing the observer an exceedingly close view of minute structures at a scale convenient for examination and analysis 1878: A mathematical theory linking resolution to light wavelength is invented by Ernst Abbe. 1903: Richard Zsigmondy invents the ultramicroscope, which allows for observation of specimens below the wavelength of light. 1932: Transparent biological materials are studied for the first time using Frits Xernike's invention of the phase-contrast microscope. 1938: Just six years after the invention of the phase contrast microscope comes the electron microscope, developed by Ernst Ruska, who realized that using electrons in microscopy enhanced resolution. 1981: 3-D specimen images possible with the invention of the scanning BRIGHT-FIELD MICROSCOPY PARTS OF THE BINOCULAR MICROSCOPE LENS SYSTEM OCULARS OBJECTIVES FINE ADJUSTMENT KNOB COARSE ADJUSTMENT KNOB ILLUMINATION SYSTEM LIGHT SOURCE CONDENSER IRIS DIAPHRAGMS BODY BASE BODY TUBE NOSEPIECE  OCULARS DESIGNED TO FURTHER MAGNIFY INCREASE MAGNIFICATION BY 10X THE OBJECT THAT HAS BEEN ENHANCED BY THE OBJECTIVES FOR VIEWING OBJECTIVES adjusted to be near the specimen and perform the initial LOW POWER, DRY 10X magnification of the object on the mechanical stage HIGH POWER, DRY 40X OIL IMMERSION 100X RESOLUTION-ABILITY OF THE LENS TO DISTINGUISH TWO SMALL OBJECTSTHAT ARE SPECIFIC DISTANCE APART  NUMERICAL APERTURE - REPRESENTS THE REFRACTIVE INDEX OF THE MATERIAL BETWEEN THE SLIDE AND THE OUTER LENS AND THE ANGLE OF THE LIGHT PASSESS THROUGH AP RESOLVING POWER COARSE AND FINE ADJUSTMENT KNOB CONTROL THE DISTANCE BETWEEN THE SLIDE AND THE OBJECTIVE PARFOCAL -REQUIRE ONLY MINIMUM ADJUSTMENT WHEN SWITCHING AMONG OBJECTIVES LIGHT SOURCE RHEOSTAT-REGULATE THE INTENSITY OF THE LIGHT DIAPHRAGM FIELD DIAPHRAGM-CONTROLS THE APERTURE DIAPHRAGM-CONTROLS THE DIAMETER OF THE LIGHT BEAM AMOUNT OF LIGHT RAYS THAT PASSESS REACHING THE SLIDE. ADJUSTED FOR TO THE SPECIMEN AND LENS FOR OPTIMAL ILLUMINATION RESOLUTION, CONTRAST AND DEPTH OF CONDENSER FOCUSES THE LIGHT ON THE SPECIMEN THE FIELD IMAGE AND CONTROLS THE LIGHT FOR UNIFORM ILLUMINATION CARE OF THE MICROSCOPE CARRY THE MICROSCOPE WITH TWO HANDS, SUPPORTING THE BASE WITH ONE HAND ALWAYS HOLD THE MICROSCOPE IN A VERTICAL POSITION ONLY CLEAN OPTICAL SURFACES WITH GOOD QUALITY LENS TISSUE AND COMMERCIAL LENS CLEANER DO NOT USE THE 10X AND 40X OBJECTIVES WITH OIL CLEAN OIL IMMERSION LENS AFTER USE ALWAYS REMOVE SLIDES WITH LOW POWER OBJECTIVE RAISED STORE THE MICROSCOPE WITH THE LOW POWER OBJECTIVE IN POSITION AND THE STAGE CENTERED CEDAR WOOD OIL-OIL IMMERSION OIL  TYPES OF MICROSCOPY (OPTICAL) BRIGHT-FIELD MICROSCOPY ROUTINE PHASE-CONTRAST MICROSCOPY LOW REFRACTIVE INDICES BIREFRINGENT-CAN REFRACT POLARIZING MICROSCOPY LIGHT IN 2 DIMENSION DARK-FIELD MICROSCOPY TREPONEMA PALLIDUM FLUORESCENT DYE (FLOUROCHROME OR FLUORESCENCE MICROSCOPY FLUOROPHORE) 3D MICROSCOPY-IMAGE AND INTERFACE-CONTRAST MICROSCOPY LAYER-BY-LAYER IMAGING UNDER THE MICROSCOPE HIGH POWER OIL IMMERSION LOW POWER FIELD FIELD FIELD REVIEW PREPARE FOR AN ORAL REVALIDA NEXT MEETING!!! 20 POINTS