XII NEET (BIO) Past Paper PDF 03.09.2024

 **Std:-** XII NEET(BIO) **Date :-** 03.09.2024 **Teacher Name:** RK Sir **Topic: Biotehnology I and DNA fingerprinting** **SECTION-A (Botany)** 101) **SECTION-B (BOTANY)** 136) **SECTION-A (ZOOLOGY)** 151\) The creation of a DNA fingerprint would normally employ all t...

 **Std:-** XII NEET(BIO) **Date :-** 03.09.2024 **Teacher Name:** RK Sir **Topic: Biotehnology I and DNA fingerprinting** **SECTION-A (Botany)** 101) **SECTION-B (BOTANY)** 136) **SECTION-A (ZOOLOGY)** 151\) The creation of a DNA fingerprint would normally employ all the following techniques except : 1\. Southern blotting 2\. Use of restriction endonucleases 3\. Gel electrophoresis 4\. Western blotting 152\) Which of the following cannot be inteferred from the information given in the following diagram : NCERT Section 1\. The DNA from the crime scene matches individual B 2\. VNTRs are hypervariable 3\. The larger fragments remain near the point on introduction in the gel electrophoresis 4\. The smaller chromosomes in the human genome have smaller repeats of VNTR 153\) Consider the following statements: I. The VNTRs belong to a class of satellite DNA called as mini- satellite. II\. Southern blotting is the transfer of DNA fragments to a filter cellulose membrane III\. VNTRs are used in DNA fingerprinting as they do not show any variations and are not inherited. Which of the above statements are true? 1\. I and II only 2\. I and III only 3\. II and III only 4\. I, II and III 154\) Satellite DNA is important because it 1\. codes for protiens needed in cell cycle 2\. shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children 3\. does not code for protiens and is same in all members of the population 4\. codes for enzymes needed for DNA replication 155\) PCR and Restriction Fragment Length Polymorphism are the methods for 1\. study of enzymes 2\. genetic transformation 3\. DNA sequencing 4\. genetic fingerprinting 156\) Satellite DNA is useful tool in \(1) organ transplantation \(2) sex determination \(3) forensic science \(4) genetic engineering 157\) Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given. A. Electrophoresis of DNA fragments B. Hybridisation with DNA probe C. Digestion of DNA by restriction endonucleases D. Autoradiography E. Blotting of NDA fragments to nitrocellulose membrane \(1) C -- A -- B -- E -- D \(2) C -- A -- E -- B -- D \(3) A -- E -- C -- B -- D \(4) A -- C -- E -- D -- B 158\) Differences in sequence of DNA make every individual unique in their (1)total number of nucleotides (2)phenotypic appearance (3)both 1 and 2 (4)None of these 159\) During density gradient centrifugation, minor peaks represent \(1) bulk DNA (2)repetitive DNA (3)Genomic DNA (4)Satellite DNA 160\) Satellite DNA classification is based on (1)Base composition (2)length of segment (3)no of repetitive units (4)All of the above 161\) Satellite DNA are \(1) Protein coding (2)M-RNA coding (3)non- protein coding (4)satellite protein coding 162\) What forms the basis of DNA fingerprinting (1)Genomic DNA (2)Bulk DNA (3)satellite DNA (4)Both 1 and 3 163\) Satellite DNA shows (1)high degree of polymorphism (2)low degree of polymorphism (3)no polymorphism (4)None of these 164\) Polymorphism in DNA forms the basis of (1)genetic mapping (2)DNA fingerprinting (3)Both 1 and 2 (4)None of these 165\) How many of these statements are true for polymorphism (A)it is inheritable (B)it arises due to mutation (C)it varies at genetic level (D)it is the basis of genetic mapping (E)it is important for evolution and speciation (F)It varies from single nucleotide to large scale \(1) 6 \(2) 5 \(3) 4 \(4) 3 166\) In DNA fingerprinting probe used is made up of (1)satellite DNA (2)genomic DNA (3)RNA (4)Protein 167\) VNTR stands for \(1) Variable number of Tendon repeats \(2) variable number for tendem repeats \(3) variable number of tandem repeats \(4) variable number of term repeats 168\) The size of VNTR varies from 1\. 0.1 to 20 kb 2\. 0.1 to 200 kb 3\. 1 to 20 kb 4\. 0.10 to.20 kb 169\) The DNA fingerprinting is same for \(1) cousins \(2) monozygotic twins \(3) Dizygotic twins \(4) siblings 170\) The DNA fingerprinting has applications in (1)forensic sciences (2)population genetics (3)genetic diversities determination (4)All of the above 171\) Father of DNA fingerprinting is \(1) H.J.Khorana \(2) Alec Jeffreys \(3) Karry Mullis \(4) E.M Southern 172\) A piece of nucleic acid used to find a gene, by forming a hybrid with it, is called a 1\. Probe 2\. Vector 3\. Restriction sequence 4\. Retrovirus 173\) The DNA profiles below represent four different individuals. Which of the following statements is consistent with the results?  1\. B is the child of A and C. 2\. C is the child of A and B. 3\. D is the child of B and C. 4\. A is the child of B and C. 174\) Allelic sequence variation where more than one variant allele at a locus in. a human population with a frequency greater than 0.01 is referred to as: 1\. DNA polymorphism 2\. Multiple allelism 3\. SNP 4\. EST 175\) Restriction endonuclease generated DNA fragments separated by gel electrophoresis and blot transferred onto a membrane filter are probed with radioactive DNA fragment. This procedure is called \(1) Gene cloning \(2) The southern technique \(3) Gene mapping \(4) PCR 176\) Restriction enzymes are synthesized by: \(1) bacteria only \(2) yeast and bacteria \(3) eukaryotic cells only \(4) all kinds of cells 177\) What limitation of traditional hybridization used for plant and animal breeding has been overcome by the use of rDNA techniques? 1\. High cost 2\. Insertion of undesirable genes 3\. Complexity of the procedure 4\. Antigenicity of the product 178\) What is true A,B and C in the given diagrammatic representation of rDNA technology ? https://base-to-link.s3.ap-south-1.amazonaws.com/Question\_question\_30850\_1616325242734.jpeg I. At A same restriction enzyme is used to cut both foreign and vector DNA II\. The enzyme used at B is DNA ligase III\. Step C can be called as transformation 1\. I and II only 2\. I and III only 3\. II and III only 4\. I,II and III 179\) What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? \(1) The larger the fragment size, the farther it moves \(2) The smaller the fragment size, the farther it moves \(3) Positively charged fragments move to farther end \(4) Negatively charged fragments do not move 180\) The cutting of DNA at specific locations became possible with the discovery of \(1) restriction enzymes \(2) probes \(3) selectable markets \(4) ligases 181\) DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by \(1) centrifugation \(2) polymerase chain reaction \(3) electrophoresis \(4) restriction mapping 182\) Introduction of foreign genes for improving genotype is 1\. biotechnology 2\. vernalization 3\. tissue culture 4\. genetic engineering 183\) In 1960's two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was 1\. Methylase 2\. Exonuclease 3\. Aminotransferase 4\. Terminal Transferase 184\) The native charge on DNA molecules is \(1) Positive \(2) Negative \(3) Neutral \(4) DNA is an ampholyte 185\) The definition of biotechnology given by EFB encompasses 1\. Traditional view 2\. Modern molecular biotechnology 3\. Both 1 and 2 4\. Only Molecular analogs 186\) Which of the following is not a core technique which gave birth to modern biotechnology? 1\. Genetic engineering 2\. Bioprocess engineering 3\. Fermentation 4\. Both 1 and 2 187\) If you transform a cell with an alien piece of DNA only, What is the possibility? 1\. This alien piece becomes a part of the host genome. 2\. It doesn\'t become a part of the host\'s genome but keeps on replicating 3\. The cloning of genes won\'t get affected in the absence of Origin of replication. 4\. Multiple identical copies of an alien piece of DNA can be formed irrespective of its integration into the host\'s genome. 188\) Cohen and Boyer are known for which of the following discoveries? 1\. Antibiotic resistance gene 2\. Recombinant DNA 3\. Restriction Digestion 4\. Plasmid 189\) Today we know more than........ restriction enzymes that have been isolated from over........strains 1\. 700; 350 2\. 900; 230 3\. 600; 270 4\. 550; 210 190\) In a DNA gel the fragment of 2kb and 3kb will be 1\. 2kb nearer to cathode while 3kb nearer to anode 2\. 2kb closer to wells while 3kb towards the opposite ends 3\. 2kb closer to opposite end of gel while 3kb closer to well 4\. 2kb away from cathode while 3kb towards anode 191\) Consider the following statements: I: Asexual reproduction preserves genetic information while sexual reproduction permits variations. II: Traditional hybridization often leads to the inclusion and multiplication of undesirable genes along with the desired genes. III: rDNA technology allows us to isolate and introduce only one or a set of desirable genes without introducing undesirable genes in the target organism. Which of the above statements are true? 1\. I and II only 2. I and III only 3\. II and III only 4. I, II, and III 192\) Which one of the following techniques made it possible to genetically engineer living organisms? 1\. Recombinant DNA techniques 2\. X-ray diffraction 3\. Heavier isotope labeling 4\. Hybridization 193\) Gene cloning occurs: 1\. when a phage transfers bacterial DNA from one bacterium to another. 2\. when a bacterium takes up DNA from the surrounding fluid. 3\. when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. 4\. when DNA is produced from an RNA template. 194\) Two enzymes responsible for restricting the growth of bacteriophages in Escherichia coil were isolated. One was methylase and other was restriction endonuclease. What was the significance of methylase? \(1) Able to cut the DNA of bacteriophage at specific sites \(2) Able to remove the methyl group and hence prevent the action of restriction endonuclease on host DNA \(3) Protection of host DNA from the action of restriction endonuclease by adding methyl group to one or two bases usually within the sequence recognised by restriction enzyme \(4) Able to ligate the two cohesive ends of DNA molecule 195\) The letter 'R' in the name of restriction endonuclease; ECoRI, denotes \(1) Genus of source \(2) Species of source \(3) Strain of source \(4) Order of discovery of source 196\) The term \'molecular scissors\' is used for \(1) Reverse transcriptase \(2) Restriction enzyme \(3) DNA polymerase \(4) RNA polymerase 197\) Which of the following performs a function which is just opposite to that performed by restriction endonuclease enzyme? \(1) DNA ligase \(2) DNA polymerase \(3) Reverse transcriptase \(4) Exonuclease 198\) Among the following, select the tools of recombinant DNA technology a\. Restriction enzymes b\. Polymerase enzymes c\. Ligases d\. Vectors e\. Host organisms 1\. a, b, c, d, and e 2\. a, c, d and e only 3\. a, b, c, and d only 4\. a, b, c and e only 199\) Read the following statements Statement-A : Restriction enzymes are obtained only from eukaryotes. Statement-B : The first discovered restriction endonuclease was EcoRI. Choose the correct option. \(1) Both statements are correct \(2) Both statements are incorrect \(3) Statement A is correct but B is incorrect \(4) Statement B is correct but A is incorrect 200\) The definition given by EFB for biotechnology is 1\. Techniques of using live organisms only 2\. Techniques of using enzymes only to produce products and processes useful to humans 3\. The integration of natural science and organisms, cells, parts there of and molecular analogues for product and services 4\. Techniques which include only synthesising a gene and using it