* What is the correct order of staining reagents in Gram-Staining? * Growth of microbes in a solid media is identified by the formation of? * The first phase of a growth curve is?... * What is the correct order of staining reagents in Gram-Staining? * Growth of microbes in a solid media is identified by the formation of? * The first phase of a growth curve is? * Autoclaving is carried out at? * What is a cluster of polar flagella called? * Optimum temperature required for the growth of the growth of bacteria is? * The iodine used in Gram staining serves as a? * Endotoxin produced by gram-negative bacteria is present in? * Temperature required for pasteurization is? * Which of the following is ionizing radiation?
Understand the Problem
The question consists of a series of multiple-choice questions related to microbiology, covering topics such as Gram staining, bacterial growth, autoclaving, flagella, temperature requirements, endotoxins, pasteurization, and ionizing radiation. Each question requires knowledge of basic microbiological concepts and laboratory techniques.
Answer
Crystal violet, iodine, decolorizer, safranin.
Based on the search results, the correct order of staining reagents in Gram staining is crystal violet (or methylene blue) as the primary stain, followed by iodine as a mordant, then a decolorizer (alcohol or acetone), and finally safranin as a counterstain.
Answer for screen readers
Based on the search results, the correct order of staining reagents in Gram staining is crystal violet (or methylene blue) as the primary stain, followed by iodine as a mordant, then a decolorizer (alcohol or acetone), and finally safranin as a counterstain.
More Information
Gram staining is a differential staining technique used to classify bacteria into two groups: Gram-positive and Gram-negative. The procedure relies on the ability of Gram-positive bacteria to retain the crystal violet stain during the decolorization process, while Gram-negative bacteria lose the crystal violet stain and are subsequently counterstained with safranin.
Tips
A common mistake is not timing the decolorization step correctly, which can lead to false Gram-positive results if the alcohol is left on for too short a time, or false Gram-negative results if left on for too long.
Sources
- Gram Staining - StatPearls - NCBI Bookshelf - ncbi.nlm.nih.gov
- [PDF] oto - gacbe.ac.in
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