What can be concluded if the right upper quadrant of a FACS plot shows only dead cells?
Understand the Problem
The question is asking about the interpretation of findings in a FACS plot, specifically regarding the significance of observing only dead cells in the right upper quadrant. It is essential to understand the implications of cell viability in the context of biological processes such as autophagy, apoptosis, necrosis, and mitosis.
Answer
The right upper quadrant showing only dead cells suggests staining by a dye like PI, which marks cells with compromised membranes.
If the right upper quadrant of a FACS plot shows only dead cells, this could indicate the use of a fluorochrome that stains cell components accessible due to membrane permeabilization in dead cells, such as PI, which only penetrates dead cells.
Answer for screen readers
If the right upper quadrant of a FACS plot shows only dead cells, this could indicate the use of a fluorochrome that stains cell components accessible due to membrane permeabilization in dead cells, such as PI, which only penetrates dead cells.
More Information
Dead cells are often characterized by compromised cell membranes, allowing certain dyes to penetrate and fluoresce. The right upper quadrant in a FACS plot typically indicates cells that have high-fluorescence staining, characteristic of dyes that penetrate only dead cells.
Tips
A common mistake is failing to use appropriate controls to distinctly identify live and dead populations, leading to inaccurate data interpretation.
Sources
- Needed some help with flow cytometry - ResearchGate - researchgate.net
- Dead Cell Identification in Flow Cytometry - Thermo Fisher Scientific - thermofisher.com
- Live / Dead Cell Exclusion - Flow Cytometry Guide | Bio-Rad - bio-rad-antibodies.com
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