During DNA denaturation, what happens to the UV absorbance?
Understand the Problem
The question asks about the effect of DNA denaturation on UV absorbance. DNA denaturation involves breaking the hydrogen bonds between complementary strands, resulting in single-stranded DNA. The question explores how this change in structure affects the way DNA absorbs UV light.
Answer
During DNA denaturation, UV absorbance increases, known as the hyperchromic effect.
During DNA denaturation, the UV absorbance increases. This phenomenon is known as the hyperchromic effect/shift. The increase in absorbance is typically measured at a wavelength of 260 nm.
Answer for screen readers
During DNA denaturation, the UV absorbance increases. This phenomenon is known as the hyperchromic effect/shift. The increase in absorbance is typically measured at a wavelength of 260 nm.
More Information
The hyperchromic effect is due to the increased exposure of the purine and pyrimidine bases when DNA is denatured. These bases absorb UV light strongly. Denaturation can be induced by heat, changes in pH, or by certain chemicals.
Tips
A common misconception is that DNA denaturation decreases UV absorbance. It's crucial to remember that the opposite is true: denaturation increases UV absorbance.
Sources
- Why does denatured DNA absorb more ultraviolet light than native DNA? - promega.com
- DNA Denaturation - an overview | ScienceDirect Topics - sciencedirect.com
- Why does denaturation of DNA increase UV absorption? - Vaia - vaia.com
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