Zwitterions and Buffers Quiz
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Questions and Answers

What is the purpose of the Henderson-Hasselbalch equation?

  • To calculate the pH of a buffer solution (correct)
  • To quantify the pH change upon addition of a large amount of base
  • To determine the pKa value of a strong acid
  • To evaluate the basicity of a conjugate base

    • What does the isoelectric point (pI) represent for amino acids?

  • The pH where amino acids have no net electrical charge (correct)
  • The point where amino acids are at their highest basicity
  • The point where amino acids exhibit the highest buffer effectiveness
  • The pH at which amino acids act as strong acids

    • Why is it important to choose a buffer with a pKa close to the desired pH of the solution?

  • To maximize the acidic proton loss potential
  • To ensure the solution becomes highly acidic
  • To maintain the buffer's effectiveness in resisting pH changes (correct)
  • To increase the buffer's basicity

    • Which of the following is true about polyprotic acids?

    <p>They can donate more than two protons per molecule</p> Signup and view all the answers

    In what pH range is a buffer solution typically effective?

    <p>+/- 1 pH unit of its pKa</p> Signup and view all the answers

    What type of acid is H2SO4 based on its ability to donate protons?

    <p>Diprotic acid</p> Signup and view all the answers

    What is the role of zwitterions in a compound?

    <p>Having both a positive and negative charge</p> Signup and view all the answers

    How do buffers minimize pH changes in a system?

    <p>By reacting with OH⁻ ions</p> Signup and view all the answers

    What is the effective pH range of a buffer considered to be?

    <p>pKa ± 1</p> Signup and view all the answers

    How does buffer capacity relate to the concentration of buffer components?

    <p>Higher concentration increases buffer capacity</p> Signup and view all the answers

    Why is it important to select a buffer with a pKa close to the desired pH?

    <p>To ensure effective buffering</p> Signup and view all the answers

    What factor should be considered in selecting a suitable buffer for a study?

    <p>Buffer compatibility with the process</p> Signup and view all the answers

    Which of the following buffers would be most suitable for maintaining a slightly acidic pH in biochemical studies?

    <p>Acetic Acid/Acetate Buffer</p> Signup and view all the answers

    At which pH range is the Phosphate Buffer most effective?

    <p>pH 6-8</p> Signup and view all the answers

    Which of the following buffers is suitable for alkaline conditions?

    <p>Tris Buffer</p> Signup and view all the answers

    What does a lower pKa value indicate about an acid?

    <p>It's stronger</p> Signup and view all the answers

    Why is the Tris Buffer often used in DNA extraction and gel electrophoresis?

    <p>It has a pKa of 8.1 at 25°C</p> Signup and view all the answers

    In which pH range do functional groups with lower pKa values lose protons first according to the text?

    <p>Low pH range</p> Signup and view all the answers

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