Zwitterions and Buffers Quiz

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What is the purpose of the Henderson-Hasselbalch equation?

To calculate the pH of a buffer solution (correct)

To quantify the pH change upon addition of a large amount of base

To determine the pKa value of a strong acid

To evaluate the basicity of a conjugate base

What does the isoelectric point (pI) represent for amino acids?

The pH where amino acids have no net electrical charge (correct)

The point where amino acids are at their highest basicity

The point where amino acids exhibit the highest buffer effectiveness

The pH at which amino acids act as strong acids

Why is it important to choose a buffer with a pKa close to the desired pH of the solution?

To maximize the acidic proton loss potential

To ensure the solution becomes highly acidic

To maintain the buffer's effectiveness in resisting pH changes (correct)

To increase the buffer's basicity

Which of the following is true about polyprotic acids?

They can donate more than two protons per molecule Signup and view all the answers

In what pH range is a buffer solution typically effective?

+/- 1 pH unit of its pKa Signup and view all the answers

What type of acid is H2SO4 based on its ability to donate protons?

Diprotic acid Signup and view all the answers

What is the role of zwitterions in a compound?

Having both a positive and negative charge Signup and view all the answers

How do buffers minimize pH changes in a system?

By reacting with OH⁻ ions Signup and view all the answers

What is the effective pH range of a buffer considered to be?

pKa ± 1 Signup and view all the answers

How does buffer capacity relate to the concentration of buffer components?

Higher concentration increases buffer capacity Signup and view all the answers

Why is it important to select a buffer with a pKa close to the desired pH?

To ensure effective buffering Signup and view all the answers

What factor should be considered in selecting a suitable buffer for a study?

Buffer compatibility with the process Signup and view all the answers

Which of the following buffers would be most suitable for maintaining a slightly acidic pH in biochemical studies?

Acetic Acid/Acetate Buffer Signup and view all the answers

At which pH range is the Phosphate Buffer most effective?

pH 6-8 Signup and view all the answers

Which of the following buffers is suitable for alkaline conditions?

Tris Buffer Signup and view all the answers

What does a lower pKa value indicate about an acid?

It's stronger Signup and view all the answers

Why is the Tris Buffer often used in DNA extraction and gel electrophoresis?

It has a pKa of 8.1 at 25°C Signup and view all the answers

In which pH range do functional groups with lower pKa values lose protons first according to the text?

Low pH range Signup and view all the answers

Zwitterions and Buffers Quiz

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Questions and Answers

What is the purpose of the Henderson-Hasselbalch equation?

What does the isoelectric point (pI) represent for amino acids?

Why is it important to choose a buffer with a pKa close to the desired pH of the solution?

Which of the following is true about polyprotic acids?

In what pH range is a buffer solution typically effective?

What type of acid is H2SO4 based on its ability to donate protons?

What is the role of zwitterions in a compound?

How do buffers minimize pH changes in a system?

What is the effective pH range of a buffer considered to be?

How does buffer capacity relate to the concentration of buffer components?

Why is it important to select a buffer with a pKa close to the desired pH?

What factor should be considered in selecting a suitable buffer for a study?

Which of the following buffers would be most suitable for maintaining a slightly acidic pH in biochemical studies?

At which pH range is the Phosphate Buffer most effective?

Which of the following buffers is suitable for alkaline conditions?

What does a lower pKa value indicate about an acid?

Why is the Tris Buffer often used in DNA extraction and gel electrophoresis?

In which pH range do functional groups with lower pKa values lose protons first according to the text?

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