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Questions and Answers

Which biomarker is associated with squamous cell carcinoma of the lung?

  • CYFRA 21-1 (correct)
  • Chromogranin A
  • Histaminase
  • Ferritin

Which of the following is a marker for adrenal or pituitary cancer?

  • Gastrin
  • Insulin-like growth factor
  • Metanephrines
  • DHEA (correct)

Which biomarker indicates the presence of multiple myeloma?

  • IgA (correct)
  • Ferritin
  • Galactosyltransferase
  • P 21 protein

What is the specific cancer marker for ovarian cancer?

<p>Galactosyltransferase (D)</p> Signup and view all the answers

Which of the following markers is associated with choriocarcinoma?

<p>HCG (intact mol) (A)</p> Signup and view all the answers

How is the presence of pheochromocytoma indicated?

<p>Metanephrines (A)</p> Signup and view all the answers

Which marker is linked to breast cancer?

<p>P 21 protein (B)</p> Signup and view all the answers

Which biomarker is associated with acute myelocytic leukemia?

<p>Ferritin (C)</p> Signup and view all the answers

What characterizes a malignant neoplasm?

<p>Anaplasia and potential for metastases (B)</p> Signup and view all the answers

What causes cancer at the cellular level?

<p>Mutations in proto-oncogenes and tumor-suppressor genes (A)</p> Signup and view all the answers

Which of the following does NOT typically apply to benign neoplasms?

<p>Potential for metastasis (C)</p> Signup and view all the answers

Which of the following is an indication of an immune response to a tumor?

<p>Mononuclear response to tumor cells (C)</p> Signup and view all the answers

What does the term 'anaplasia' refer to in tumor biology?

<p>Abnormal tissue structure and loss of differentiation (C)</p> Signup and view all the answers

Which type of neoplasm is likely to cause death due to its invasive nature?

<p>Malignant neoplasm (D)</p> Signup and view all the answers

What is the definition of a tumor in the context of tumor biology?

<p>An abnormal mass of tissue that grows uncontrollably (D)</p> Signup and view all the answers

What evidence supports the immune response against tumors?

<p>Spontaneous regression of tumors (D)</p> Signup and view all the answers

What is the primary role of immunosurveillance in the immune system?

<p>Destroying cancer cells before they are clinically evident. (A)</p> Signup and view all the answers

Which cells are considered part of the innate immune defenses against tumors?

<p>Natural killer (NK) cells. (C)</p> Signup and view all the answers

What characterizes active immunotherapy?

<p>The stimulation of the patient's immune system to respond to tumor antigens. (A)</p> Signup and view all the answers

What is an example of passive immunotherapy?

<p>Cytokines like IL-2. (A)</p> Signup and view all the answers

What do monoclonal antibodies specifically target in passive immunotherapy?

<p>Tumor antigens, surface receptors, and angiogenesis factors. (A)</p> Signup and view all the answers

Which of the following best describes cytokines in the context of immunotherapy?

<p>They nonspecifically boost the immune response. (D)</p> Signup and view all the answers

Which type of immunotherapy includes cancer vaccines?

<p>Active immunotherapy. (B)</p> Signup and view all the answers

Coley’s toxins and BCG are examples of what type of immunotherapy?

<p>Active immunotherapy. (A)</p> Signup and view all the answers

What can cause false positives in serum tumor marker detection?

<p>Heterophile antibodies (A)</p> Signup and view all the answers

Which type of immunotherapy involves the infusion of tumor-infiltrating lymphocytes (TILs)?

<p>Adoptive immunotherapy (A)</p> Signup and view all the answers

What type of relationship is characterized by both host and microbes benefiting each other?

<p>Mutualistic (D)</p> Signup and view all the answers

What is a key feature of automated immunoassays for detecting serum tumor markers?

<p>They are sensitive and may produce false positives. (B)</p> Signup and view all the answers

Which of the following is NOT a form of immunotherapy?

<p>Bacterial culture (D)</p> Signup and view all the answers

What is a characteristic of a commensalistic relationship between host and microbes?

<p>One organism benefits while the other is not affected. (C)</p> Signup and view all the answers

Which of these bacteria is commonly associated with virulence factors related to gastric ulcers?

<p>Helicobacter pylori (B)</p> Signup and view all the answers

What type of relationship involves host and microbes coexisting without long-term benefits or harms?

<p>Commensalistic (C)</p> Signup and view all the answers

What is the significant titer for the Widal test indicating a strong possibility of typhoid fever?

<p>1:80 (C)</p> Signup and view all the answers

What type of antibodies does the Typhidot test detect for recent and remote infections?

<p>IgG and IgM (C)</p> Signup and view all the answers

What is a primary limitation of the Typhidot test?

<p>It provides only qualitative results. (B)</p> Signup and view all the answers

Which antigen is associated with active or current infections in the Widal test?

<p>O antigen (C)</p> Signup and view all the answers

What do a positive IgM result from the Typhidot test indicate?

<p>Recent infection (B)</p> Signup and view all the answers

For what type of fever is the combination of O and A or B antigens significant?

<p>Mixed infection (D)</p> Signup and view all the answers

Which antigen is characterized as a somatic antigen in Salmonella paratyphi?

<p>A and B antigens (C)</p> Signup and view all the answers

What does the Weil-Felix test specifically diagnose?

<p>Typhus fever (A)</p> Signup and view all the answers

What type of bacterium is Helicobacter pylori?

<p>Gram-negative, urease-producing (B)</p> Signup and view all the answers

Which method is NOT commonly used to diagnose H. pylori infection?

<p>Blood culture (A)</p> Signup and view all the answers

Which of the following is a characteristic of Mycoplasma pneumoniae?

<p>It lacks a cell wall (C)</p> Signup and view all the answers

What is the gold standard for diagnosing Rocky Mountain Spotted Fever (RMSF)?

<p>Serologic testing for antibodies to R. rickettsii via indirect fluorescent antibody (IFA) (A)</p> Signup and view all the answers

What type of infections are mainly caused by Mycoplasma pneumoniae?

<p>Respiratory infections (C)</p> Signup and view all the answers

What is a common complication of untreated H. pylori infection?

<p>Gastric carcinoma (B)</p> Signup and view all the answers

What type of bacteria are Rickettsia?

<p>Obligate intracellular, gram-negative (C)</p> Signup and view all the answers

Which of the following is NOT a method to detect Mycoplasma pneumoniae infection?

<p>Blood culture using standard media (A)</p> Signup and view all the answers

Flashcards

Neoplasm

New growth of cells that doesn't normally induce tissue multiplication.

Benign Neoplasm

Slow-growing, localized tumor, not usually fatal.

Malignant Neoplasm

Rapid or slow-growing tumor with spread and potential to cause death.

Cancer

Malignant tumor that can spread throughout the body.

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Tumor

Abnormal cell mass, sometimes benign, sometimes malignant.

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Tumor Biology

Study of how tumor cells behave, grow, and interact with the body.

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Tumor Antigen

Unique proteins on tumor cells recognized by the immune system.

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Immune Response Evidence (Tumors)

Signs that show the body's immune system is fighting the tumor.

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Chromogranin A

A protein marker associated with pheochromocytoma.

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CYFRA 21-1

A marker for squamous cell carcinoma of the lung.

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DHEA

A hormone linked to adrenal or pituitary cancer.

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Metanephrines

Biochemical markers associated with pheochromocytoma.

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Neuron-specific enolase

A marker for small cell lung cancer.

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PNP

A protein found in leukemia.

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Ferritin

A protein linked to acute myelocytic leukemia.

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HCG (intact mol)

A marker for choriocarcinoma.

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Immunosurveillance

The immune system's constant monitoring of the body for cancer cells and their elimination before they become a threat.

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Innate Defenses Against Tumors

Natural defenses that act quickly to kill tumor cells, including NK cells and macrophages.

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Adaptive Immune Responses Against Tumors

Targeted and specific immune responses that eliminate tumor cells, involving CTLs, dendritic cells, cytokines, and antibodies.

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Immunotherapy for Cancer

Treatment that uses the body's own immune system to fight cancer.

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Active Immunotherapy

Stimulating the patient's immune system to respond to tumor antigens.

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Passive Immunotherapy

Administering components of the immune system to directly fight cancer cells.

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Monoclonal Antibodies in Cancer Therapy

Targeted antibodies that bind to specific cancer cell antigens, leading to their destruction.

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Cytokines in Cancer Therapy

Proteins that enhance the immune response, such as GM-CSF, IL-2, and IFN-a, used in passive immunotherapy to fight cancer.

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Widal Test

A blood test used to detect antibodies against Salmonella typhi and paratyphi, indicating typhoid fever or past infection.

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O Antigen

A somatic antigen found on the surface of Salmonella bacteria, indicating active or current typhoid infection.

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H Antigen

A flagellar antigen found on Salmonella bacteria, suggesting past infection, recent immunizations, or exposure.

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Significant Titer

A Widal test result indicating a high concentration of antibodies, usually 1:80 or greater with at least +2 reaction.

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A or B Antigen

Indicates paratyphoid fever caused by Salmonella paratyphi A or B.

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O and A or B

Indicates a mixed infection of Salmonella typhi and Salmonella paratyphi.

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Typhidot Test

A rapid diagnostic test for typhoid fever that detects IgM and IgG antibodies against the outer membrane protein (OMP) of Salmonella typhi.

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50Kd OMP Antigen

A specific protein found on the outer membrane of Salmonella typhi, targeted by the Typhidot test.

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Host-microbe Relationship

The interaction between a host organism and microbes, such as bacteria. This can be symbiotic (beneficial to both), commensalistic (neutral), mutualistic (beneficial to both), or parasitic (harmful to the host).

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Indigenous Microbiota

The community of microbes (mostly bacteria) that normally live in a particular environment of the host, such as the gut. This community can be beneficial to host health.

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Bacterial Virulence Factors

Structures or molecules produced by bacteria that allow them to cause disease by evading the host's immune system, damaging tissues or causing disease directly.

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Immune Defenses Against Bacteria

Different parts of the immune system, like antibodies, white blood cells, and physical barriers, that work together to combat bacterial infections.

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Bacterial Infection Detection

Methods used to identify bacterial infections in a lab, like culturing bacteria from samples or detecting specific bacterial components using serological or molecular techniques.

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Group A Streptococci

A type of bacteria that can cause various infections, such as strep throat, skin infections, and scarlet fever.

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Helicobacter Pylori

A type of bacteria that infects the stomach and is associated with gastritis, ulcers, and stomach cancer.

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Mycoplasma Pneumoniae

A type of bacteria that lacks a cell wall and causes atypical pneumonia, a lung infection.

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H. pylori Infection

Caused by a gram-negative bacterium that produces urease, leading to ulcers, gastric carcinoma, and MALT tumors.

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H. pylori Diagnosis

Can be diagnosed through urease detection in biopsy tissue, the urea breath test, or ELISA for H. pylori antibodies.

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M. pneumoniae Infection

Caused by tiny bacteria lacking a cell wall, known for causing respiratory infections like 'walking pneumonia'.

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M. pneumoniae Detection

Diagnosed using serological assays for antibodies or PCR for bacterial DNA; cold agglutinins are present in about half of patients.

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Rickettsia (RMSF and Typhus)

Obligate intracellular bacteria transmitted by arthropods, causing diseases like Rocky Mountain Spotted Fever (RMSF) and Typhus.

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Rickettsia Diagnosis

The gold standard for RMSF diagnosis is serological testing for antibodies using IFA.

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Syphilis

Caused by Treponema pallidum, a spirochete bacterium, and transmitted through sexual contact.

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Lyme Disease

Caused by Borrelia burgdorferi, a spirochete bacterium, transmitted through tick bites.

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Study Notes

Immunodeficiency Diseases

  • Primary immunodeficiencies (PIDs) are inherited while secondary immunodeficiencies are acquired.
  • PIDs result from missing or dysfunctional immune system components.
  • PIDs make individuals more susceptible to infections and cancer.
  • Several immune system components can be affected by PIDs including humoral immunity, cell-mediated immunity and innate immunity.
  • Humoral immunodeficiencies are caused by defects in B cells or Th cells.
  • Cell-mediated immunodeficiencies involve T-cell deficiencies.
  • Innate immunodeficiencies involve neutrophils or macrophages, or complement.
  • Nine categories of PIDs: Combined immunodeficiencies, combined immunodeficiencies with associated or syndromic features, predominantly antibody deficiencies, diseases of immune dysregulation, congenital defects of phagocytes, defects in innate immunity, autoinflammatory disorders, complement deficiencies, and phenocopies of primary immunodeficiencies.
  • Several types and causes of PID are mentioned, including X-linked, autosomal recessive, and autosomal dominant inheritance.
  • Some disorders, like X-linked severe combined immunodeficiency (XSCID), are caused by mutations in specific genes, such as the IL2RG gene.
  • Many types of laboratory tests can be used to diagnose PIDs, including flow cytometry to assess white blood cell (WBC) counts and types.
  • Laboratory detection of primary immunodeficiencies is done through various tests.

Tumor Immunology

  • Tumor biology encompasses the properties of a tumor, including malignant properties, uncontrolled cell division, and the ability to resist apoptosis.
  • Tumor cells often evade normal immune defenses and can form a mass, causing swelling.
  • Tumor antigens are proteins or enzymes unique to tumors.
  • Clinically relevant tumor markers are biological substances found in elevated amounts in bodily fluids or tissues for certain cancers.
  • Laboratory tests for tumors such as immunohistochemistry, immunoassays, molecular methods, and proteomics can detect antigens.
  • Immunosurveillance refers to the immune system's role in recognizing and destroying tumor cells.
  • Examples of tumor markers include AFP, CA 125, and CEA.
  • Several types of immunotherapies, including active, passive, and adoptive, exist for the treatment of cancer.
  • Active immunotherapy stimulates the patient's immune system.
  • Passive immunotherapy administers soluble components of the immune system.
  • Adoptive immunotherapy involves transferring immune cells to patients.

Serologic and Molecular Detection of Certain Bacterial Infections

  • Host-microbe relationships involve symbiosis, commensalism, mutualism, and parasitism.
  • Bacterial infectivity is the organism's ability to establish an infection.
  • Bacterial pathogenicity is the ability of an organism to cause disease.
  • Bacterial virulence describes the extent of the pathology caused by an organism.
  • Structures of bacteria include cell wall, cytoplasm membrane, capsule, pili, plasmids, and flagellum.
  • Virulence factors of bacteria include endotoxins and exotoxins (toxins released from live bacteria).
  • Immune defenses against bacteria include innate defenses (skin, mucosal surfaces, antimicrobial peptides, complement proteins) and adaptive defenses (antibodies, cell-mediated immunity).
  • Laboratory detection of bacterial infections includes culture, microscopic examination, detection of bacterial antigens, and molecular detection techniques.
  • Various clinical manifestations are associated with specific bacterial infections, and laboratory tests are used to detect and diagnose those infections.
  • Group A streptococcal infections, Helicobacter pylori infections, Mycoplasma pneumoniae infections, rickettsial infections, and certain serologic tests are highlighted, including ASO, anti-DNase, and Widals tests.

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