Yersinia Diagnostic Laboratory Tests

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10 Questions

What is the preferred method of collecting stool specimens for the recovery of vibrios?

Collecting stools early in the course of the diarrheal illness

What is the characteristic motility observed in V.cholerae O1 under dark-field or phase-contrast microscopy?

Shooting star motility

What is the primary purpose of using a polyvalent O1 antiserum in detecting V.cholerae O1?

To confirm the identity of the organism

What is the temperature required for incubating agar medium for isolating C.fetus?

36–37°C

What is the primary method of diagnosing H.pylori infection?

Histologic examination of gastric biopsy specimens

What specimen is typically taken for culture in cases of possible meningitis?

Cerebrospinal fluid

Which staining method may be more useful for examining material from suspected buboes or positive blood cultures?

Fluorescent antibody stains

What temperature favors the growth of Y.pestis on culture media?

28°C

Which of the following tests is likely to be positive within 24 hours in cases like Y.pestis infection?

Blood culture

What is the best method for definitive identification of Y.pestis cultures?

Immunofluorescence or lysis by specific bacteriophage

Explore the diagnostic laboratory tests used in Yersinia infection cases, including different specimens and staining methods. Learn about the culture of blood, aspirates, sputum, and cerebrospinal fluid, as well as the examination of acute and convalescent sera for antibody levels.

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