Yersinia Diagnostic Laboratory Tests
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Questions and Answers

What is the preferred method of collecting stool specimens for the recovery of vibrios?

  • Collecting stools through rectal swabs
  • Collecting stools early in the course of the diarrheal illness (correct)
  • Collecting stools during the later stages of diarrheal illness
  • Mixing stool specimens with a polyvalent O1 antiserum

What is the characteristic motility observed in V.cholerae O1 under dark-field or phase-contrast microscopy?

  • Random motility
  • Gull wing motility
  • Darting motility
  • Shooting star motility (correct)

What is the primary purpose of using a polyvalent O1 antiserum in detecting V.cholerae O1?

  • To enhance the motility of the organism
  • To inhibit the growth of the organism
  • To confirm the identity of the organism (correct)
  • To detect the presence of antibodies

What is the temperature required for incubating agar medium for isolating C.fetus?

<p>36–37°C (C)</p> Signup and view all the answers

What is the primary method of diagnosing H.pylori infection?

<p>Histologic examination of gastric biopsy specimens (C)</p> Signup and view all the answers

What specimen is typically taken for culture in cases of possible meningitis?

<p>Cerebrospinal fluid (D)</p> Signup and view all the answers

Which staining method may be more useful for examining material from suspected buboes or positive blood cultures?

<p>Fluorescent antibody stains (B)</p> Signup and view all the answers

What temperature favors the growth of Y.pestis on culture media?

<p>28°C (D)</p> Signup and view all the answers

Which of the following tests is likely to be positive within 24 hours in cases like Y.pestis infection?

<p>Blood culture (B)</p> Signup and view all the answers

What is the best method for definitive identification of Y.pestis cultures?

<p>Immunofluorescence or lysis by specific bacteriophage (A)</p> Signup and view all the answers

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