Podcast
Questions and Answers
What is the typical copy number of YCp yeast centromere plasmid vectors per cell?
What is the typical copy number of YCp yeast centromere plasmid vectors per cell?
- 1 to 3 (correct)
- 10 to 15
- 20 to 25
- 5 to 7
Which type of selection markers complement an auxotrophic mutation in the genome of yeast?
Which type of selection markers complement an auxotrophic mutation in the genome of yeast?
- Dominant selection markers
- Antibiotic markers
- Complementation markers (correct)
- Marker genes
Which yeast promoter mentioned in the text is constitutive in expression?
Which yeast promoter mentioned in the text is constitutive in expression?
- TRPI
- SUC2
- GAPDH (correct)
- ADH2
What are the two types of yeast selection markers mentioned in the text?
What are the two types of yeast selection markers mentioned in the text?
What are the YCp yeast centromere plasmid vectors primarily used for?
What are the YCp yeast centromere plasmid vectors primarily used for?
Which of the following is NOT a type of yeast selection marker mentioned in the text?
Which of the following is NOT a type of yeast selection marker mentioned in the text?
What kind of replication sequences do YCp yeast centromere plasmid vectors contain?
What kind of replication sequences do YCp yeast centromere plasmid vectors contain?
What is the function of dominant selection markers in yeast?
What is the function of dominant selection markers in yeast?
What advantages do yeast cell factories offer for recombinant protein production?
What advantages do yeast cell factories offer for recombinant protein production?
Why is the engineering of yeast glycosylation to produce glycoproteins of human-like glycan structures of great interest?
Why is the engineering of yeast glycosylation to produce glycoproteins of human-like glycan structures of great interest?
How can the expression of human proteins in yeast be utilized in drug screens?
How can the expression of human proteins in yeast be utilized in drug screens?
What is the significance of using yeast cell factories for recombinant protein production?
What is the significance of using yeast cell factories for recombinant protein production?
How can the yeast cell factory's secretory pathway contribute to recombinant protein production?
How can the yeast cell factory's secretory pathway contribute to recombinant protein production?
What is one of the advantages of the Pichia expression system mentioned in the text?
What is one of the advantages of the Pichia expression system mentioned in the text?
What makes the P. pastoris expression system unique in the production of certain proteins?
What makes the P. pastoris expression system unique in the production of certain proteins?
How does the P. pastoris expression system contribute to the ease of recombinant protein purification?
How does the P. pastoris expression system contribute to the ease of recombinant protein purification?
What is the significance of P. pastoris being a methylotrophic yeast?
What is the significance of P. pastoris being a methylotrophic yeast?
What role does the Pichia expression system play in the production of recombinant proteins?
What role does the Pichia expression system play in the production of recombinant proteins?
What is a unique feature of Pichia expression system regarding the secretory production of recombinant proteins?
What is a unique feature of Pichia expression system regarding the secretory production of recombinant proteins?
Which type of glycosylation is characterized by the attachment of oligosaccharides to serine or threonine residues?
Which type of glycosylation is characterized by the attachment of oligosaccharides to serine or threonine residues?
In which cellular organelle does N-linked glycosylation primarily take place in yeast cells?
In which cellular organelle does N-linked glycosylation primarily take place in yeast cells?
What is the consensus sequence for the attachment of oligosaccharides through N-glycosyl linkage to asparagine residues in yeast cells?
What is the consensus sequence for the attachment of oligosaccharides through N-glycosyl linkage to asparagine residues in yeast cells?
Which type of glycosylation is typical of the hypermannose type in yeast cells?
Which type of glycosylation is typical of the hypermannose type in yeast cells?
What type of saccharides are typically much smaller than N-linked saccharides found in S. cerevisiae?
What type of saccharides are typically much smaller than N-linked saccharides found in S. cerevisiae?
Which organism does not hyperglycosylate therapeutic proteins and does not contain potentially immunogenic terminal α-1,3-linked mannoses?
Which organism does not hyperglycosylate therapeutic proteins and does not contain potentially immunogenic terminal α-1,3-linked mannoses?
What plays an important role in achieving complete biological activities of therapeutic proteins such as interferon, erythropoietin, and monoclonal antibodies?
What plays an important role in achieving complete biological activities of therapeutic proteins such as interferon, erythropoietin, and monoclonal antibodies?
What can lead to immunologic reaction and decreased serum half-life in the P. pastoris expression system?
What can lead to immunologic reaction and decreased serum half-life in the P. pastoris expression system?
Pichia GlycoS is a new strategy designed for engineering which type of glycosylation pathway in P. pastoris?
Pichia GlycoS is a new strategy designed for engineering which type of glycosylation pathway in P. pastoris?
S. cerevisiae core oligosaccharides have terminal α‐1,3 glycan linkages whereas P. pastoris does not. What is primarily responsible for the hyper‐antigenic nature of proteins produced from S. cerevisiae?
S. cerevisiae core oligosaccharides have terminal α‐1,3 glycan linkages whereas P. pastoris does not. What is primarily responsible for the hyper‐antigenic nature of proteins produced from S. cerevisiae?
