Yeast YCp Vectors and Selection Markers Quiz

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What is the typical copy number of YCp yeast centromere plasmid vectors per cell?

1 to 3 (correct)

10 to 15

20 to 25

5 to 7

Which type of selection markers complement an auxotrophic mutation in the genome of yeast?

Dominant selection markers

Antibiotic markers

Complementation markers (correct)

Marker genes

Which yeast promoter mentioned in the text is constitutive in expression?

TRPI

SUC2

GAPDH (correct)

ADH2

What are the two types of yeast selection markers mentioned in the text?

Dominant selection and complementation Signup and view all the answers

What are the YCp yeast centromere plasmid vectors primarily used for?

Cloning vectors Signup and view all the answers

Which of the following is NOT a type of yeast selection marker mentioned in the text?

SUC2 Signup and view all the answers

What kind of replication sequences do YCp yeast centromere plasmid vectors contain?

Autonomously replicating sequences (ARS) Signup and view all the answers

What is the function of dominant selection markers in yeast?

Antibiotic resistance Signup and view all the answers

What advantages do yeast cell factories offer for recombinant protein production?

Fast growth, easy genetic manipulation, and eukaryotic features Signup and view all the answers

Why is the engineering of yeast glycosylation to produce glycoproteins of human-like glycan structures of great interest?

To produce glycoproteins with human-like glycan structures Signup and view all the answers

How can the expression of human proteins in yeast be utilized in drug screens?

It can lead to an arrest in cell growth and division Signup and view all the answers

What is the significance of using yeast cell factories for recombinant protein production?

They combine fast growth, easy genetic manipulation, and eukaryotic features Signup and view all the answers

How can the yeast cell factory's secretory pathway contribute to recombinant protein production?

By leading to correct protein processing and post-translational modifications Signup and view all the answers

What is one of the advantages of the Pichia expression system mentioned in the text?

Inexpensive and relatively rapid expression times Signup and view all the answers

What makes the P. pastoris expression system unique in the production of certain proteins?

It is suitable for the production of membrane proteins such as calcium and potassium channels Signup and view all the answers

How does the P. pastoris expression system contribute to the ease of recombinant protein purification?

By limiting the production of endogenous secretory proteins Signup and view all the answers

What is the significance of P. pastoris being a methylotrophic yeast?

It allows for inexpensive and relatively rapid expression times Signup and view all the answers

What role does the Pichia expression system play in the production of recombinant proteins?

It enables the production of recombinant proteins on a large scale from small culture volumes Signup and view all the answers

What is a unique feature of Pichia expression system regarding the secretory production of recombinant proteins?

It facilitates direct secretion into the supernatant of the culture medium Signup and view all the answers

Which type of glycosylation is characterized by the attachment of oligosaccharides to serine or threonine residues?

O-linked glycosylation Signup and view all the answers

In which cellular organelle does N-linked glycosylation primarily take place in yeast cells?

Endoplasmic reticulum Signup and view all the answers

What is the consensus sequence for the attachment of oligosaccharides through N-glycosyl linkage to asparagine residues in yeast cells?

Asn-X-Ser/Thr Signup and view all the answers

Which type of glycosylation is typical of the hypermannose type in yeast cells?

N-linked glycosylation Signup and view all the answers

What type of saccharides are typically much smaller than N-linked saccharides found in S. cerevisiae?

GlcNAc2 Signup and view all the answers

Which organism does not hyperglycosylate therapeutic proteins and does not contain potentially immunogenic terminal α-1,3-linked mannoses?

P. pastoris Signup and view all the answers

What plays an important role in achieving complete biological activities of therapeutic proteins such as interferon, erythropoietin, and monoclonal antibodies?

N-acetylglucosamine addition Signup and view all the answers

What can lead to immunologic reaction and decreased serum half-life in the P. pastoris expression system?

Hypermannosylation Signup and view all the answers

Pichia GlycoS is a new strategy designed for engineering which type of glycosylation pathway in P. pastoris?

N-glycosylation pathway Signup and view all the answers

S. cerevisiae core oligosaccharides have terminal α‐1,3 glycan linkages whereas P. pastoris does not. What is primarily responsible for the hyper‐antigenic nature of proteins produced from S. cerevisiae?