Lecture 13 – RNA splicing
29 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary role of introns in eukaryotic genes?

  • They code for proteins.
  • They are regulatory sequences.
  • They are non-coding regions that are removed during RNA processing. (correct)
  • They enhance transcription.

    • What is the first step in the process of gene expression?

  • RNA splicing
  • Translation
  • Transcription (correct)
  • RNA processing

    • Which technique was pivotal in discovering the existence of split genes?

  • Gel electrophoresis
  • R-loop analysis (correct)
  • Northern blotting
  • Western blotting

    • Which of the following statements about group I introns is true?

    <p>They can self-splice in vitro.</p> Signup and view all the answers

    In R-loop analysis, what does a single displaced loop of ssDNA indicate?

    <p>That the gene is contiguous with no introns.</p> Signup and view all the answers

    What role does the 5' splice site play in RNA splicing?

    <p>It is where the intron is cleaved.</p> Signup and view all the answers

    What are exons?

    <p>Coding sequences that remain in the mature mRNA after splicing.</p> Signup and view all the answers

    How does the presence of an extra G at the 5' end of an intron influence splicing?

    <p>It serves as a nucleophile in the splicing reaction.</p> Signup and view all the answers

    What is a characteristic feature of group II introns?

    <p>They can self-splice and are found in organelles.</p> Signup and view all the answers

    What is the function of splicing factors in spliceosome-dependent splicing?

    <p>To assist in the recognition and removal of introns.</p> Signup and view all the answers

    What is the role of the spliceosome in RNA splicing?

    <p>It provides a framework for the splicing process.</p> Signup and view all the answers

    What does the term "alternative splicing" refer to?

    <p>The generation of multiple protein isoforms from a single gene.</p> Signup and view all the answers

    Which of the following components is NOT part of the spliceosome?

    <p>RNA polymerase II</p> Signup and view all the answers

    What is the significance of the branch site in group II introns?

    <p>It participates in the formation of a lariat structure during splicing.</p> Signup and view all the answers

    Which type of intron is characterized by the requirement of ATP and a splicing endonuclease for its removal?

    <p>Nuclear tRNA introns</p> Signup and view all the answers

    In the context of intron evolution, what does the "intron early hypothesis" suggest?

    <p>Introns are of ancient origin and have been maintained due to their functional importance.</p> Signup and view all the answers

    In the splicing mechanism of group II introns, what does the 2'-OH of the branch site adenosine do?

    <p>It attacks the phosphate at the 5' splice site.</p> Signup and view all the answers

    What is the main difference between group I and group II introns?

    <p>Group I introns do not have a branch site, while group II introns do.</p> Signup and view all the answers

    What is the primary consequence of spliceosome-mediated splicing for eukaryotic cells?

    <p>Enhanced efficiency and regulation of gene expression.</p> Signup and view all the answers

    Which of the following is a characteristic of spliceosomal introns?

    <p>They require specific snRNPs for splicing.</p> Signup and view all the answers

    Signup and view all the answers

    Which of the following best describes transesterification in RNA splicing?

    <p>A chemical reaction that exchanges functional groups between RNA molecules</p> Signup and view all the answers

    What is the role of the phosphorylated C-terminal domain of RNA polymerase II in splicing?

    <p>It recruits spliceosomes, coordinating splicing with transcription.</p> Signup and view all the answers

    Which of the following mechanisms allows for the generation of multiple protein isoforms from a single gene?

    <p>All of the abov</p> Signup and view all the answers

    What is the purpose of the R-loop analysis technique?

    <p>To determine the presence of introns in a gene.</p> Signup and view all the answers

    Which type of RNA is primarily involved in the splicing of introns in spliceosome-dependent splicing?

    <p>snRNA</p> Signup and view all the answers

    What is the significance of the conserved features found in introns?

    <p>They facilitate the recognition of splice sites during splicing.</p> Signup and view all the answers

    Signup and view all the answers

    In the context of intron evolution, what is meant by the "intron late hypothesis"?

    <p>Introns are a recent development that arose after the evolution of eukaryotic complexity.</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser