Lecture 13 – RNA splicing

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Questions and Answers

What is the primary role of introns in eukaryotic genes?

  • They code for proteins.
  • They are regulatory sequences.
  • They are non-coding regions that are removed during RNA processing. (correct)
  • They enhance transcription.

What is the first step in the process of gene expression?

  • RNA splicing
  • Translation
  • Transcription (correct)
  • RNA processing

Which technique was pivotal in discovering the existence of split genes?

  • Gel electrophoresis
  • R-loop analysis (correct)
  • Northern blotting
  • Western blotting

Which of the following statements about group I introns is true?

<p>They can self-splice in vitro. (B)</p> Signup and view all the answers

In R-loop analysis, what does a single displaced loop of ssDNA indicate?

<p>That the gene is contiguous with no introns. (B)</p> Signup and view all the answers

What role does the 5' splice site play in RNA splicing?

<p>It is where the intron is cleaved. (A)</p> Signup and view all the answers

What are exons?

<p>Coding sequences that remain in the mature mRNA after splicing. (B)</p> Signup and view all the answers

How does the presence of an extra G at the 5' end of an intron influence splicing?

<p>It serves as a nucleophile in the splicing reaction. (B)</p> Signup and view all the answers

What is a characteristic feature of group II introns?

<p>They can self-splice and are found in organelles. (A)</p> Signup and view all the answers

What is the function of splicing factors in spliceosome-dependent splicing?

<p>To assist in the recognition and removal of introns. (B)</p> Signup and view all the answers

What is the role of the spliceosome in RNA splicing?

<p>It provides a framework for the splicing process. (B)</p> Signup and view all the answers

What does the term "alternative splicing" refer to?

<p>The generation of multiple protein isoforms from a single gene. (B)</p> Signup and view all the answers

Which of the following components is NOT part of the spliceosome?

<p>RNA polymerase II (B)</p> Signup and view all the answers

What is the significance of the branch site in group II introns?

<p>It participates in the formation of a lariat structure during splicing. (C)</p> Signup and view all the answers

Which type of intron is characterized by the requirement of ATP and a splicing endonuclease for its removal?

<p>Nuclear tRNA introns (D)</p> Signup and view all the answers

In the context of intron evolution, what does the "intron early hypothesis" suggest?

<p>Introns are of ancient origin and have been maintained due to their functional importance. (B)</p> Signup and view all the answers

In the splicing mechanism of group II introns, what does the 2'-OH of the branch site adenosine do?

<p>It attacks the phosphate at the 5' splice site. (C)</p> Signup and view all the answers

What is the main difference between group I and group II introns?

<p>Group I introns do not have a branch site, while group II introns do. (C)</p> Signup and view all the answers

What is the primary consequence of spliceosome-mediated splicing for eukaryotic cells?

<p>Enhanced efficiency and regulation of gene expression. (B)</p> Signup and view all the answers

Which of the following is a characteristic of spliceosomal introns?

<p>They require specific snRNPs for splicing. (C)</p> Signup and view all the answers

Signup and view all the answers

Which of the following best describes transesterification in RNA splicing?

<p>A chemical reaction that exchanges functional groups between RNA molecules (B)</p> Signup and view all the answers

What is the role of the phosphorylated C-terminal domain of RNA polymerase II in splicing?

<p>It recruits spliceosomes, coordinating splicing with transcription. (B)</p> Signup and view all the answers

Which of the following mechanisms allows for the generation of multiple protein isoforms from a single gene?

<p>All of the abov (D)</p> Signup and view all the answers

What is the purpose of the R-loop analysis technique?

<p>To determine the presence of introns in a gene. (B)</p> Signup and view all the answers

Which type of RNA is primarily involved in the splicing of introns in spliceosome-dependent splicing?

<p>snRNA (C)</p> Signup and view all the answers

What is the significance of the conserved features found in introns?

<p>They facilitate the recognition of splice sites during splicing. (B)</p> Signup and view all the answers

Signup and view all the answers

In the context of intron evolution, what is meant by the "intron late hypothesis"?

<p>Introns are a recent development that arose after the evolution of eukaryotic complexity. (B)</p> Signup and view all the answers

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